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PubMed · 7920981

Do barbiturates impair zymosan-induced granulocyte function?

M Weiss·R Buhl·N Mirow·A Birkhahn·M Schneider·P Wernet
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Abstract

PURPOSE: The dose-response relationship of commercially available preparations of methohexital, pentobarbital, phenobarbital, and thiopental and their respective drug-free solutions on granulocyte function was investigated to evaluate whether suppression of neutrophil chemiluminescence is mediated by the barbiturates themselves or by their drug-free solutions. Furthermore, it was assessed whether suppression of chemiluminescence is due to an interaction mainly with neutrophils or to free radical scavenging. METHODS: The dose-response effects of the four barbiturates on granulocyte function were tested by zymosan-induced neutrophil chemiluminescence and, in addition, in a cell-free chemiluminescence system. RESULTS: Methohexital and pentobarbital did not influence zymosan-induced neutrophil chemiluminescence, whereas phenobarbital and thiopental decreased neutrophil chemiluminescence in a dose-dependent fashion. Nonphysiological osmolality (531 mosmol/kg) caused this impaired neutrophil chemiluminescence at the greatest concentration of phenobarbital. Thiopental solely suppressed neutrophil chemiluminescence drug specifically. Because thiopental also reduced chemiluminescence generated in a cell-free system, free radical scavenging might contribute to the impaired neutrophil chemiluminescence observed with thiopental. CONCLUSIONS: With the exception of thiopental, barbiturates do not impair oxygen radical production during phagocytosis of neutrophils.

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Cell-Free SystemDose-Response Relationship, DrugFree Radical ScavengersGranulocytesHumansLuminescent MeasurementsMethohexitalN-Formylmethionine Leucyl-PhenylalanineOsmolar ConcentrationPentobarbitalPhagocytosisPhenobarbitalSolutionsThiopentalZymosanJournal Article

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M Weiss, R Buhl, N Mirow, A Birkhahn, M Schneider, P Wernet. 1994. Do barbiturates impair zymosan-induced granulocyte function?. https://doi.org/10.1016/0883-9441(94)90018-3

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