Pseudorabies virus growth factor can be resolved into two active components.

J Gasperík; J Lesko; M Csabayová; F Golais

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PubMed · 7976863

Pseudorabies virus growth factor can be resolved into two active components.

J Gasperík·J Lesko·M Csabayová·F Golais

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Abstract

Pseudorabies virus (PRV) growth factor (PRGF) which induces a transformed phenotype in normal MK-2 cells and represses the transformed phenotype of Hela cells was partially purified and resolved into two components (M(r) < 300 and < 180). Each of the PRGF components retained the transforming activity of the original factor in MK-2 cells but lost its transformation-repressing activity in Hela cells. The latter activity of PRGF could be reconstituted by simultaneous application of its two components. Two monoclonal antibodies against gII glycoprotein of PRV were able to neutralize both PRGF activities, thus supporting the previously suggested hypothesis that the PRV gene for glycoprotein gII might be involved in PRGF synthesis.

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Animals Cell Line Cell Transformation, Viral Growth Substances Haplorhini HeLa Cells Herpesvirus 1, Suid Humans Phenotype Journal Article Research Support, Non-U.S. Gov't

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J Gasperík, J Lesko, M Csabayová, F Golais. 1994. Pseudorabies virus growth factor can be resolved into two active components.. https://pubmed.ncbi.nlm.nih.gov/7976863/

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