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Two improved methods for preparing ferritin-protein conjugates for electron microscopy.

Y Kishida·B R Olsen·R A Berg·D J Prockop
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Abstract

Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin-antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.

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AnimalsAntibodiesAntigen-Antibody ReactionsCarbodiimidesChromatography, GelFerritinsGlutaralGoatsImmunochemistryImmunoelectrophoresisImmunoglobulin Fab FragmentsImmunoglobulin GImmunologic TechniquesIndicators and ReagentsIodidesIodine RadioisotopesMicroscopy, ElectronRabbitsSuccinimidesJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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Y Kishida, B R Olsen, R A Berg, D J Prockop. 1975. Two improved methods for preparing ferritin-protein conjugates for electron microscopy.. https://doi.org/10.1083/jcb.64.2.331

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