[Studies on knee function and injuries].

J Gillqvist

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PubMed · 8121786

[Studies on knee function and injuries].

J Gillqvist

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Abstract

The development of arthroscopy for diagnosis and treatment has resulted in improvement in the treatment of knee joint disease and injury. Despite technical improvements, however, reconstruction of cruciate ligaments is still far from obtaining optimal results, and techniques for promoting the healing of cartilage defects are still lacking.

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Anterior Cruciate Ligament Anterior Cruciate Ligament Injuries Arthroscopy Humans Knee Injuries Knee Joint Menisci, Tibial Posterior Cruciate Ligament Prostheses and Implants Range of Motion, Articular Tibial Meniscus Injuries Journal Article

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J Gillqvist. 1994. [Studies on knee function and injuries].. https://pubmed.ncbi.nlm.nih.gov/8121786/

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PubMed1995

Adhesiveness of human ligament fibroblasts to laminin.

The adhesiveness of fibroblasts from the human anterior cruciate and medial collateral ligaments to the laminin molecule was studied, with particular emphasis on the intrinsic differences between fibroblasts from the two ligaments. Cellular adhesion strength, adhesion area, laminin concentration, and seeding time were examined. Cell adhesion to laminin anchored with poly-D-lysine to a cleaned cover glass was measured with a micropipette micromanipulation system after seeding. The adhesion strength of fibroblasts from the anterior cruciate ligament to laminin was greater than and significantly different from that of fibroblasts from the medial collateral ligament, depending on the laminin concentration. Fibroblasts from the anterior cruciate ligament also exhibited an increase in adhesion strength, dependent on laminin concentration of as much as 30 micrograms/ml, at which the laminin receptors were thought to be saturated. Fibroblasts from the medial collateral ligament did not show such an increase except at laminin concentrations of 5-10 micrograms/ml. There was no significant difference in adhesion area between fibroblasts from the two ligaments except after 45 minutes at a laminin concentration of 40 micrograms/ml. For both, the adhesion to laminin showed little correlation to seeding time during periods of as long as 60 minutes. Measurements of adhesion area also failed to show a significant correlation to seeding time for fibroblasts from either ligament at laminin concentrations of 20 and 40 micrograms/ml. Adhesion strength normalized by adhesion area had no correlation to seeding time.(ABSTRACT TRUNCATED AT 250 WORDS)

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