A modified method for tissue localization of cells bearing a complement receptor.

C J Meijer; J Lindeman

doi:10.1016/0022-1759(75)90035-6

Home/Archive/PubMed

PubMed · 812919

A modified method for tissue localization of cells bearing a complement receptor.

C J Meijer·J Lindeman

View original source ↗Export metadata ↓

Abstract

A modification of the method originally described by Dukor et al. (1970) was used for localization of cells bearing a complement receptor in lymphoid tissue of man, guinea pig, mouse and rat. Optimal fixation of the adherent indicator erythrocytes to the tissue section and satisfactory morphology of the tissue were obtained with a mixture of 1% paraformaldehyde and 0.5% glutaraldehyde. In addition, selective staining of indicator erythrocyte hemoglobin with Amido Black resulted in high contrast with the tissue constituents. The combination of fixative and stain descirbed above permitted more precise localization of cells bearing complement receptors in tissue sections.

Explore related subjects

Amido Black Animals Binding Sites, Antibody Complement System Proteins Formaldehyde Glutaral Guinea Pigs Humans Immune Adherence Reaction Lymph Nodes Lymphoid Tissue Mice Palatine Tonsil Rats Receptors, Drug Spleen Thymus Gland Journal Article

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

C J Meijer, J Lindeman. 1975. A modified method for tissue localization of cells bearing a complement receptor.. https://doi.org/10.1016/0022-1759(75)90035-6

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2005

High-efficiency staining of proteins on different blot membranes.

Explore the source record for details and available documents.

Amido Black↗

PubMed2005

Textile dye decolorization using cyanobacteria.

Cyanobacterial cultures isolated from sites polluted by industrial textile effluents were screened for their ability to decolorize cyclic azo dyes. Gloeocapsa pleurocapsoides and Phormidium ceylanicum decolorized Acid Red 97 and FF Sky Blue dyes by more than 80% after 26 days. Chroococcus minutus was the only culture which decolorized Amido Black 10B (55%). Chlorophyll a synthesis in all cultures was strongly inhibited by the dyes. Visible spectroscopy and TLC confirmed that color removal was due to degradation of the dyes.

Amido Black↗

PubMed2005

Low-temperature inhibition of the activated sludge process by an industrial discharge containing the azo dye acid black 1.

A municipal wastewater treatment plant (WWTP) receiving industrial dyeing discharge containing acid black 1 (AB1) failed to meet NH(3) and BOD(5) discharge limits, especially for NH(3) during the winter. Dyeing discharge was combined with domestic sewage in volumetric ratios reflecting the range received by the WWTP and fed to sequencing batch reactors at 22 and 7 degrees C. Analysis of the various nitrogen species revealed complete nitrification failure at 7 degrees C with more rapid nitrification failure as the dye concentration increased. Slight nitrification inhibition occurred at 22 degrees C: NH(3) removal decreased from 99.9% for the control compared to only 97.0% removal with dye addition. Dye-bearing wastewater also reduced COD removal by half at 7 degrees C and by one-fifth at 22 degrees C, and increased effluent TSS nearly three-fold at 7 degrees C. Activated sludge quality at 7 degrees C deteriorated after exposure to AB1, as indicated by excessive foaming and the presence of filamentous bacteria and by a decrease in endogenous and exogenous oxygen uptake. Decreasing AB1 loading resulted in partial activated sludge recovery. Eliminating the dye-bearing discharge to the full-scale WWTP led to improved performance bringing the WWTP into compliance with discharge limits.

Amido Black↗