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PubMed · 8271834

A simple method for recording single-channel activity from synaptic plasma membranes.

A C Hall·G R Tibbs·J O Dolly·W R Lieb·N P Franks
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Abstract

Due to the small size of most nerve terminals, the ion channels which underlie presynaptic currents are usually inaccessible to investigation by conventional electrophysiological techniques. Here we describe a simple method for obtaining single-channel recordings from synaptic plasma membranes that does not require exposure of the native membranes to exogenous lipids or fusogens. To illustrate the method, we have recorded single-channel activity from rat cerebrocortical synaptosomal membranes. Under conditions designed to isolate calcium-independent currents, we describe three channel types that are most commonly observed.

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Action PotentialsAnimalsNeurophysiologyPotassium ChannelsProsencephalonRatsRats, Sprague-DawleySynaptic MembranesSynaptic TransmissionJournal ArticleResearch Support, Non-U.S. Gov't

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A C Hall, G R Tibbs, J O Dolly, W R Lieb, N P Franks. 1993. A simple method for recording single-channel activity from synaptic plasma membranes.. https://doi.org/10.1016/0165-0270(93)90111-4

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