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PubMed · 8342928

Irradiation effects on wound contraction using a connective tissue model.

A Yanase·M Ueda·T Kaneda·S Torii·M Matsuyama
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Abstract

To analyze irradiation effects on wound contraction, fibroblasts harvested from human oral mucosa were irradiated (single 2-, 4-, 6-, 8-, and 10-Gy exposures of x-rays). The irradiated fibroblasts were examined for growth ability, and they were cultured three-dimensionally in hydrated collagen gels. The irradiation inhibited growth of fibroblasts and contraction of collagen gels in a dose-dependent manner. In addition, inhibition depended on the duration after exposure. The organization of actin filaments of fibroblasts in this model was examined with fluorescent dye-conjugated phalloidin. Many elongated cell projections were formed in cells of the control group, but the cell projections were almost disrupted in the irradiated cells. X-ray irradiation is thus shown to inhibit wound contraction by affecting the organization of actin filaments in fibroblasts.

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ActinsCells, CulturedCollagenCytoskeletonDose-Response Relationship, RadiationFibroblastsHumansIn Vitro TechniquesMouth MucosaWound HealingJournal ArticleResearch Support, Non-U.S. Gov't

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A Yanase, M Ueda, T Kaneda, S Torii, M Matsuyama. 1993. Irradiation effects on wound contraction using a connective tissue model.. https://doi.org/10.1097/00000637-199305000-00007

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