E-cadherin expression in bladder cancer using formalin-fixed, paraffin-embedded tissues: correlation with histopathological grade, tumour stage and survival.

K N Syrigos; T Krausz; J Waxman; H Pandha; G Rowlinson-Busza; J Verne; A A Epenetos; M Pignatelli

doi:10.1002/ijc.2910640603

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PubMed · 8550236

E-cadherin expression in bladder cancer using formalin-fixed, paraffin-embedded tissues: correlation with histopathological grade, tumour stage and survival.

K N Syrigos·T Krausz·J Waxman·H Pandha·G Rowlinson-Busza·J Verne·A A Epenetos·M Pignatelli

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Abstract

To determine the potential prognostic value of epithelial cadherin (E-cadherin), a Ca(2+)-dependent cell-cell adhesion molecule, we have analysed its immunoreactivity and cellular localisation in 67 transitional cell carcinomas (TCC) using an avidin-biotin immunoperoxidase technique on formalin-fixed, paraffin-embedded tissues. These results were correlated with histopathological grade, tumour stage, presence of metastases and survival. In addition, 10 cystitis and 11 normal bladder biopsies were evaluated as controls. E-cadherin was expressed in a normal membranous pattern in all normal and 7 of 10 cystitis biopsies. Loss of normal surface E-cadherin expression was found in 3 of 15 superficial tumours and in 48 of 52 invasive cancers. Abnormal immunoreactivity was strictly related to tumour differentiation and stage. Fifteen of 20 well-differentiated (grade I) tumours showed preserved membranous E-cadherin immunoreactivity, while 46 of 47 moderate and poorly differentiated tumours (grades II and III) demonstrated abnormal staining patterns. Loss of membranous E-cadherin immunoreactivity was also associated with advanced tumour stage. There was a significantly higher 5-year survival rate for patients with preserved membranous staining compared with patients with abnormal staining.

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Cadherins Carcinoma, Transitional Cell Formaldehyde Humans Neoplasm Staging Predictive Value of Tests Survival Analysis Tissue Embedding Tissue Fixation Urinary Bladder Neoplasms Journal Article Research Support, Non-U.S. Gov't

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K N Syrigos, T Krausz, J Waxman, H Pandha, G Rowlinson-Busza, J Verne, A A Epenetos, M Pignatelli. 1995-12-20. E-cadherin expression in bladder cancer using formalin-fixed, paraffin-embedded tissues: correlation with histopathological grade, tumour stage and survival.. https://doi.org/10.1002/ijc.2910640603

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