Reverse transcriptase from avian myeloblastosis virus.

G E Houts; M Miyagi; C Ellis; D Beard; J W Beard

doi:10.1128/jvi.29.2.517-522.1979

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Reverse transcriptase from avian myeloblastosis virus.

G E Houts·M Miyagi·C Ellis·D Beard·J W Beard

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Abstract

From lots of 20 to 30 g of avian myeloblastosis virus RNA-dependent DNA polymerase was obtained in preparations of purity greater than 95% by using a two-step column chromatographic procedure employing DEAE (DE 52) and carboxymethylcellulose (CM 52.). Yields of RNA-dependent DNA polymerase varied from approximately 20,000 to 35,000 U/g of virus. Specific activity of the enzyme was about 35,000 to 60,000 U/mg of protein. Free of detectable RNase activity, the product exhibited a molecular weight of about 160,000, an isoelectric point of 6.5, and approximately 2 mol of fatty acid per mol of enzyme.

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Animals Avian Leukosis Avian Leukosis Virus Avian Myeloblastosis Virus Blood Chickens Fatty Acids Glycoproteins Isoelectric Point Molecular Weight RNA-Directed DNA Polymerase Viral Proteins Journal Article Research Support, U.S. Gov't, P.H.S.

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G E Houts, M Miyagi, C Ellis, D Beard, J W Beard. 1979. Reverse transcriptase from avian myeloblastosis virus.. https://doi.org/10.1128/jvi.29.2.517-522.1979

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