Development of a parallel plate flow chamber for studying cell behavior under pulsatile flow.

J Ruel; J Lemay; G Dumas; C Doillon; J Charara

PubMed · 8589470

Development of a parallel plate flow chamber for studying cell behavior under pulsatile flow.

J Ruel·J Lemay·G Dumas·C Doillon·J Charara

Abstract

The design of a new parallel plate perfusion chamber for cell behavior studies involving pulsatile flowrates is presented. It was based on fluid mechanical considerations to ensure a region of regular and uniform shear stress at the wall. A numeric solution of the flow was performed to study the effect of pulsating flow on the entrance length. Dye injection investigations in the chamber showed laminar and uniform flow in the culture region under steady state conditions.

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Cell Adhesion Cell Wall Cells, Cultured Microscopy, Phase-Contrast Models, Biological Perfusion Pulsatile Flow Stress, Mechanical Journal Article Research Support, Non-U.S. Gov't

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J Ruel, J Lemay, G Dumas, C Doillon, J Charara. Development of a parallel plate flow chamber for studying cell behavior under pulsatile flow.. https://pubmed.ncbi.nlm.nih.gov/8589470/

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