Complement activation by IgG immobilized on methylated silicon.

P Tengvall; A Askendal; I Lundström

doi:10.1002/(sici)1097-4636(199607)31:3<305::aid-jbm3>3.0.co;2-q

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Complement activation by IgG immobilized on methylated silicon.

P Tengvall·A Askendal·I Lundström

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Abstract

Activation of the complement system by immobilized IgG on methylated silicon was studied by ellipsometry/antibody-, ELISA-, and RIA techniques after exposure to human serum at 37 degrees C for up to 1 h. The IgG-covered surfaces rapidly activated the complement system and the combined results suggest an initial classical pathway activation. Complement factor 1q (C1q) and IgG were antibody-detectable on the surfaces for serum incubations up to 5 min but not thereafter. Anti-C3c and anti-properdin bound to the surfaces at all serum incubation times. Experiments with 125I-IgG preadsorbed to surfaces, or added to normal-, EGTA-, and EDTA-sera, showed that IgG was not displaced from the protein film by serum.

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CD4 Antigens Chemical Phenomena Chemistry, Physical Complement Activation Complement C3c Enzyme-Linked Immunosorbent Assay Humans Immunoenzyme Techniques Immunoglobulin G Indicators and Reagents Iodine Radioisotopes Methylation Radioimmunoassay Silicon Compounds Surface Properties Journal Article Research Support, Non-U.S. Gov't

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P Tengvall, A Askendal, I Lundström. 1996. Complement activation by IgG immobilized on methylated silicon.. https://doi.org/10.1002/(sici)1097-4636(199607)31%3A3%3C305%3A%3Aaid-jbm3%3E3.0.co%3B2-q

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