X-ray diffraction pattern from internal structure of bovine rhodopsin.

T Kawaguchi; T Hamanaka; Y Kito

doi:10.1093/oxfordjournals.jbchem.a021254

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X-ray diffraction pattern from internal structure of bovine rhodopsin.

T Kawaguchi·T Hamanaka·Y Kito

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Abstract

Pure bovine rhodopsin pellets were prepared by removal of cholic acid from rhodopsin-cholic acid complex, and X-ray diffraction patterns from the internal structure of rhodopsin were obtained for the first time using wet and dry pellet samples. The peaks at around 10 and 4.3 A spacings observed for both samples can be attributed to alpha-helices in rhodopsin molecule, providing direct evidence of the helical structure of rhodopsin. A shoulder peak at around 34 A spacing was also observed for the dry pellet, which can be explained by the first-neighbor distance between rhodopsin molecules in the sample.

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Animals Cattle Cholic Acid Cholic Acids Protein Conformation Rhodopsin X-Ray Diffraction Journal Article

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T Kawaguchi, T Hamanaka, Y Kito. 1996. X-ray diffraction pattern from internal structure of bovine rhodopsin.. https://doi.org/10.1093/oxfordjournals.jbchem.a021254

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