Bovine coronavirus nucleocapsid protein processing and assembly.

B G Hogue

doi:10.1007/978-1-4615-1899-0_41

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Bovine coronavirus nucleocapsid protein processing and assembly.

B G Hogue

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Abstract

The coronavirus nucleocapsid protein (N) encapsidates the genomic RNA to form a helical nucleocapsid. The requirements for coronavirus nucleocapsid assembly are being studied. Two forms (approximately 50 kDa and 55 kDa) of the bovine coronavirus (BCV) N protein were detected in infected cells. However, only one form, a 50 kDa species, was detected in extracellular virions. After treatment with calf intestinal alkaline phosphatase (CIAP), the 55 kDa intracellular form increased in mobility to comigrate with the 50 kDa form; whereas, the 50 kDa intracellular species and N from extracellular virions was not sensitive to CIAP treatment. The data indicate that specificity exists with regard to assembly of N into the mature virion. The data suggests that processing of N may take place during assembly of either nucleocapsids or virions and that the processing may be a dephosphorylation event.

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Alkaline Phosphatase Animals Capsid Cattle Cell Line Coronavirus, Bovine Cricetinae Intestines Kidney Molecular Weight Protein Processing, Post-Translational Protein Structure, Secondary RNA, Viral Viral Core Proteins Virion Comparative Study Journal Article

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B G Hogue. 1995. Bovine coronavirus nucleocapsid protein processing and assembly.. https://doi.org/10.1007/978-1-4615-1899-0_41

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