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PubMed · 8843663

Selectivity, differential mobility and resolution as parameters to optimize capillary electrophoretic separation.

V Dolník
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Abstract

Selectivity, differential mobility, and resolution have been tested as the optimization functions to find the optimum pH of operational electrolyte for separation by capillary electrophoresis when organic acids occurring in human serum have been selected as a model mixture for computer simulations. Using tabulated values of ionic mobilities and pKa values, either selectivity or differential mobility or resolution for the hardest-to-separate pair of separands are calculated and plotted vs. pH. The optimum pH is the pH value, at which the optimization function reaches its maximum.

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AnionsCarboxylic AcidsCitric AcidComputer SimulationElectrolytesElectrophoresis, CapillaryHumansHydrogen-Ion ConcentrationIsoelectric PointKetoglutaric AcidsLactic AcidMalatesModels, ChemicalOsmolar ConcentrationPhosphatesPyruvic AcidJournal Article

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V Dolník. 1996-09-13. Selectivity, differential mobility and resolution as parameters to optimize capillary electrophoretic separation.. https://doi.org/10.1016/0021-9673(96)00449-9

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