[Optimization of Brucella detection using polymerase chain reaction].

S V Balakhonov; M Iu Shestopalov; A I Kalinovskiĭ

PubMed · 8999321

[Optimization of Brucella detection using polymerase chain reaction].

S V Balakhonov·M Iu Shestopalov·A I Kalinovskiĭ

Abstract

The possibility of using polymerase chain reaction (PCR) for the laboratory diagnosis of brucellosis was tested with a representative regional collection of 44 Brucella strains. The strains differed by the test and source of isolation, virulence, and phenotypical characteristics. Optimal parameters of PCR have been defined and the possibility of detecting the agents in pathological material from cattle demonstrated.

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Animals Brucella Brucellosis Cattle Cattle Diseases Polymerase Chain Reaction English Abstract Journal Article

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S V Balakhonov, M Iu Shestopalov, A I Kalinovskiĭ. [Optimization of Brucella detection using polymerase chain reaction].. https://pubmed.ncbi.nlm.nih.gov/8999321/

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