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Bone morphogenetic protein 2 transcripts in rapidly developing deer antler tissue contain an extended 5' non-coding region arising from a distal promoter.

J Q Feng·D Chen·N Ghosh-Choudhury·J Esparza·G R Mundy·S E Harris
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Abstract

To understand the regulation of the BMP-2 gene expression, we recently isolated the BMP-2 gene from a mouse genomic library and characterized the exon-intron structure and promoter. RNase protection assay using poly (A)+ RNA of mouse osteoblasts demonstrates that two regions in BMP-2 gene are protected by antisense mouse BMP-2 RNA probes. These results demonstrate that BMP-2 gene utilizes two alternative promoters, a distal and a proximal promoter. In the present study we demonstrate that BMP-2 mRNA from rapidly growing deer antler tissue has an extended 5' non-coding region compared with the human and rat BMP-2 mRNA. The extended 5' non-coding region in the deer mRNA represents transcripts from the upstream distal promoter. This is the first evidence of a natural BMP-2 mRNA from a bone-forming tissue that most likely initiated from the distal transcription start site.

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Alternative SplicingAmino Acid SequenceAnimalsAntlersBase SequenceBone Morphogenetic Protein 2Bone Morphogenetic ProteinsDeerExonsGene Expression Regulation, DevelopmentalGenomic LibraryHumansIntronsMiceMolecular Sequence DataOsteoblastsPromoter Regions, GeneticRNA ProbesRatsSequence Homology, Amino AcidTranscription, GeneticTransforming Growth Factor betaComparative StudyJournal Article

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J Q Feng, D Chen, N Ghosh-Choudhury, J Esparza, G R Mundy, S E Harris. 1997-01-03. Bone morphogenetic protein 2 transcripts in rapidly developing deer antler tissue contain an extended 5' non-coding region arising from a distal promoter.. https://doi.org/10.1016/s0167-4781(96)00178-9

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