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Development of a quantitative sampling method for periapical exudates from human root canals.

H Shimauchi·Y Miki·S Takayama·T Imai·H Okada
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Abstract

A quantitative method for collection of periapical exudates on endodontic paper points is described. In a pilot study, it was revealed that the relationship between fluid volume and wetted length of paper points had a highly significant curvilinear relationship (p < 0.0001), and a highly significant positive linear relationship was found to describe eluted and absorbed interleukin (IL)-1 beta activities from paper points. Periapical exudates from 29 root canals with apical periodontitis were collected using this method, and IL-1 beta activities in clinical specimens were measured. Periapical exudates (0.15 to 26.7 microliters) were recovered, and the range of IL-1 beta concentration was 0.1 to 179.5 ng/ml. These results showed that this sampling method was useful to analyze immunological changes in periapical lesions.

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Dental Pulp CavityExudates and TransudatesGingival Crevicular FluidHumansInflammation MediatorsInterleukin-1PaperPeriapical PeriodontitisPilot ProjectsSpecimen HandlingStatistics, NonparametricJournal Article

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H Shimauchi, Y Miki, S Takayama, T Imai, H Okada. 1996. Development of a quantitative sampling method for periapical exudates from human root canals.. https://doi.org/10.1016/s0099-2399(96)80032-x

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