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Large specimen bone embedment and cement line staining.

H E Gruber·A A Stasky
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Abstract

Undecalcified embedment of large bone specimens is often challenging. A method is presented here that is suitable for methacrylate embedment of sections of canine vertebrae while retaining the ability to localize tartrate-resistant acid phosphatase and alkaline phosphatase activity. Specimens also retained tetracycline labelling, and sectioned preparations were readily stained with routine bone procedures. A modification of the Bodian silver stain, used for examining the nerves and spinal cord in these specimens, provided a useful stain for canaliculi and cement lines in trabecular and cortical bone. This stain is advantageous when both bone and nerve tissue are of interest, as in spinal fusion studies.

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AnimalsBone and BonesDogsMethacrylatesPlastic EmbeddingSilver StainingTetracyclineJournal Article

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H E Gruber, A A Stasky. 1997. Large specimen bone embedment and cement line staining.. https://doi.org/10.3109/10520299709082238

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