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Gene transfer using replication-defective human foamy virus vectors.

P D Bieniasz·O Erlwein·A Aguzzi·A Rethwilm·M O McClure
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Abstract

Replication-defective vectors based on an infectious molecular clone of human foamy virus (HFV) were constructed by deletion and replacement of the accessory genes with expression cassettes for puromycin-resistance and beta-glucouronidase. Cell lines which produced in excess of 10(5) helper virus-free transducing units/ml were generated by trans-complementation of the replication defect using a BHK-21-derived cell line expressing the Bel-1 transactivator. Vectors based on the HFV genome may provide useful alternatives to existing retroviral vectors.

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AnimalsCell LineCricetinaeDNA-Binding ProteinsDefective VirusesDrug Resistance, MicrobialGene Transfer TechniquesGenes, ReporterGenetic VectorsGlucuronidaseHumansKidneyRecombinant Fusion ProteinsRetroviridae ProteinsSpumavirusTrans-ActivatorsTransfectionVirus ReplicationJournal ArticleResearch Support, Non-U.S. Gov't

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P D Bieniasz, O Erlwein, A Aguzzi, A Rethwilm, M O McClure. 1997-08-18. Gene transfer using replication-defective human foamy virus vectors.. https://doi.org/10.1006/viro.1997.8658

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