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Antisecretory factor suppresses intestinal inflammation and hypersecretion.

E Johansson·E Jennische·S Lange·I Lönnroth
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Abstract

BACKGROUND: Antisecretory factor (AF) is a recently identified regulatory protein which inhibits the intestinal fluid secretion induced by cholera toxin. AIMS: To test the effect of AF on: (a) inflammation and hypersecretion induced by toxin A from Clostridium difficile; and (b) morphological changes and hypersecretion induced by okadaic acid (the blue mussel toxin) in rat intestinal mucosa. METHODS: Morphological changes and fluid accumulation were observed in intestinal loops challenged with 1 microgram of toxin A or 3 micrograms of okadaic acid administered before or after injection of 0.1 microgram of recombinant AF (rAF). RESULTS: The cytotoxic and inflammatory reaction caused by toxin A was abolished after treatment with rAF given either intraveneously or intraluminally prior to the toxin or one hour after the toxin. The intestinal fluid response induced by toxin A and okadaic acid was reduced 55-80% by rAF. However, the characteristic increase in goblet cells at the tips of villi in the okadaic acid treated mucosa was not inhibited by rAF. CONCLUSION: Results suggest that AF might be involved in protection against inflammation and in counteracting dehydration caused by enterotoxins. Both effects are probably mediated via the enteric nervous system.

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AnimalsAntidiarrhealsBacterial ToxinsClostridioides difficileEnterotoxinsEnzyme InhibitorsInjections, IntravenousIntestinal SecretionsIntestinesLigationMaleNeuropeptidesOkadaic AcidPhosphoprotein PhosphatasesRatsRats, Sprague-DawleyRecombinant ProteinsJournal Article

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E Johansson, E Jennische, S Lange, I Lönnroth. 1997. Antisecretory factor suppresses intestinal inflammation and hypersecretion.. https://doi.org/10.1136/gut.41.5.642

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