Protocol for microneurography with concentric needle electrodes.

Abstract

In 1968, the method of human percutaneous microneurography with solid tungsten electrodes was introduced. Since then many investigators used this technique to study peripheral mechanisms in the somatosensory, motor and autonomic systems of conscious humans. Although some modifications of the method were described, the basic construction of the recording electrode has remained the same over the years. In the present protocol we describe in detail the procedures of microneurography using a thin diameter concentric needle electrode. There are some advantages with the concentric electrodes in comparison with the tungsten needles: (1) the electrical and mechanical properties of the electrode are stable which allows repeated use, (2) its restricted and one-dimensionally directed recording area provides the possibility to study topographical aspects within even a part of a peripheral nerve fascicle, and (3) multi-channel recordings can be achieved by adding more recording surfaces to the electrode. Based on recent investigations evaluating the recording properties of concentric electrodes we propose a novel procedure for signal analysis where template matching is incorporated. The analyses described in this protocol might also be applicable for extracellular recordings from muscle or elsewhere within the nervous system.