Integrated cell isolation and polymerase chain reaction analysis using silicon microfilter chambers.

P Wilding; L J Kricka; J Cheng; G Hvichia; M A Shoffner; P Fortina

doi:10.1006/abio.1997.2530

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Integrated cell isolation and polymerase chain reaction analysis using silicon microfilter chambers.

P Wilding·L J Kricka·J Cheng·G Hvichia·M A Shoffner·P Fortina

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Abstract

White blood cells are isolated from whole blood in silicon-glass 4.5-microliter microchips containing a series of 3.5-micron feature-sized 'weir-type' filters, formed by an etched silicon dam spanning the flow chamber. Genomic DNA targets, e.g., dystrophin gene, can be directly amplified using the polymerase chain reaction (PCR) from the white cells isolated on the filters. This dual function microchip provides a means to simplify nucleic acid analyses by integrating in a single device two key steps in the analytical procedure, namely, cell isolation and PCR.

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Cell Separation DNA Dystrophin Erythrocytes Glass Hemoglobins Humans Leukocytes Micropore Filters Polymerase Chain Reaction Silicon Journal Article Research Support, Non-U.S. Gov't

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P Wilding, L J Kricka, J Cheng, G Hvichia, M A Shoffner, P Fortina. 1998-03-15. Integrated cell isolation and polymerase chain reaction analysis using silicon microfilter chambers.. https://doi.org/10.1006/abio.1997.2530

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