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PubMed · 956052

Micromonospora-produced sisomicin components.

B K Lee·R G Condon·G H Wagman·M J Weinstein·E Katz
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Abstract

A sisomicin fermentation carried out in the presence of (methyl-14C)-L-methionine resulted in a crude mixture, composed of methyl-14C-labeled sisomicin as a major component; and two 4''-C-desmethylsisomicin (66-40B and 66-40D) isomer-like components, an unidentified component and a gentamicin A-like antibiotic as minor components. When (methyl-14C)-L-methionine was added in an early stage of the fermentation (24 hours), incorporation of methyl-14C-label into polar components (e.g., gentamicin A-like antibiotic) preceded that into sisomicin. Chromatographic evidence for the bioconversion of (methyl-14C)-gentamicin A to a radioactive sisomicin-like product (possibly (3''-N-methyl-14C)-sisomicin) was seen, when a Micromonospora blocked mutant was incubated in the presence of the former antibiotic.

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Anti-Bacterial AgentsBiotransformationFermentationGentamicinsMethionineMicromonosporaSisomicinTime FactorsJournal Article

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B K Lee, R G Condon, G H Wagman, M J Weinstein, E Katz. 1976. Micromonospora-produced sisomicin components.. https://doi.org/10.7164/antibiotics.29.677

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