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PubMed · 9586052

Random amplified polymorphic DNA observed in Eucalyptus by PCR study with random primers.

M Izumi·E Kojima·M Matsuda·T Shimizu·A Murakami·T Tanabe·K Murakami
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Abstract

A total DNA extracted from Eucalyptus globlus, E. citriodora, or a subspecies of E. gunnii (Maruha Yu-kari) being used as an template, Polymerase Chain Reaction (PCR) was performed with 40 kinds of commercially available random primers (Operon, OPA-01-20 and OPB-01-20). After electrophoresis of PCR-products on agarose gel, we analyzed the band pattern formed by DNA-fragments with 0.5-3.0 kbp as molecular weight. As a remuneration for the examination in which forty 10-mer arbitrary oligonucleotides were tried to be used as primers in PCR for each species, we found that 9 primers (for E. globlus) leads polymorphism, 6 primers for E. citriodora, and 6 primers for Maruha Yu-kari.

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Base SequenceDNA PrimersDNA, PlantOperonPolymorphism, GeneticRandom Amplified Polymorphic DNA TechniqueSeedsSpecies SpecificityTreesJournal Article

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M Izumi, E Kojima, M Matsuda, T Shimizu, A Murakami, T Tanabe, K Murakami. 1997. Random amplified polymorphic DNA observed in Eucalyptus by PCR study with random primers.. https://pubmed.ncbi.nlm.nih.gov/9586052/

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