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The cyclosporine A-induced decrease in rat renal calbindin-D28kDa protein as a consequence of a decrease in its mRNA.

O Grenet·M C Varela·F Staedtler·S Steiner
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Abstract

Cyclosporine A (CsA) is a potent immunosuppressant with the drawback of renal side-effects. We recently reported that relatively high doses of CsA markedly decreased the calcium-binding protein calbindin-D28kDa in kidneys of male Wistar rats, and showed that this decrease could be associated with some of the drug-induced adverse renal effects. To investigate the events leading to this decrease, the calbindin-D28kDa mRNA level in kidneys of rats treated with 15 or 50 mg/kg/day CsA for 12 days was analysed by reverse transcription followed by polymerase chain reaction. At both doses, a marked dose-dependent decrease in the calbindin-D28kDa mRNA level was found, one very similar to the decrease measured in the calbindin-D28kDa protein abundance. Thus, the CsA-mediated down-regulation of the renal calbindin-D28kDa protein is most likely the result of a decrease in the calbindin-D28kDa mRNA level.

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AnimalsCalbindinsCyclosporineDepression, ChemicalEnzyme-Linked Immunosorbent AssayImmunosuppressive AgentsIn Vitro TechniquesKidneyMalePolymerase Chain ReactionRNA, MessengerRatsRats, WistarS100 Calcium Binding Protein GJournal Article

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O Grenet, M C Varela, F Staedtler, S Steiner. 1998-04-01. The cyclosporine A-induced decrease in rat renal calbindin-D28kDa protein as a consequence of a decrease in its mRNA.. https://doi.org/10.1016/s0006-2952(97)00646-1

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