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PubMed · 9621128

[Interference of human processed pseudogene and corresponding counter-measures in reverse transcriptase-polymerase chain reaction].

M Ge·Y Zhang·L Li·B Xi·M Wang·T Huang
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Abstract

OBJECTIVE: This study aimed to set up an effective method to find the interference of processed pseudogene and overcome it in RT-PCR. METHODS: Using RT-PCR methods, the authors amplified the cytokeratin 19 cDNA or DNA sequence in human lung squamous cell carcinoma samples. RESULTS: They could find the influence of pseudogene through performing genomic DNA and nonreversed transcribed RNA controls. By digesting the extracted RNA sample with RNase-free DNase or regulating the annealing temperature based on mismatched primers, they could effectively eliminate the interference. CONCLUSION: The presence of processed pseudogene usually interferes in the experimental protocol in RT-PCR. This problem can be resolved with the method aforementioned.

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Carcinoma, Squamous CellHumansLung NeoplasmsPolymerase Chain ReactionPseudogenesEnglish AbstractJournal Article

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M Ge, Y Zhang, L Li, B Xi, M Wang, T Huang. 1998-06-10. [Interference of human processed pseudogene and corresponding counter-measures in reverse transcriptase-polymerase chain reaction].. https://pubmed.ncbi.nlm.nih.gov/9621128/

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