High-throughput DNA sequencing on a capillary array electrophoresis system.

M Marsh; O Tu; V Dolnik; D Roach; N Solomon; K Bechtol; P Smietana; L Wang; X Li; P Cartwright; A Marks; D Barker; D Harris; J Bashkin

PubMed · 9624574

High-throughput DNA sequencing on a capillary array electrophoresis system.

M Marsh·O Tu·V Dolnik·D Roach·N Solomon·K Bechtol·P Smietana·L Wang·X Li·P Cartwright·A Marks·D Barker·D Harris·J Bashkin

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Abstract

A capillary array electrophoresis apparatus capable of running and analyzing 48 DNA sequencing samples simultaneously has been constructed. The instrument uses a replaceable sieving buffer and incorporates a convenient method for introducing the buffer into the capillaries. Data from laser-induced fluorescence are collected as four separate images, one for each optical channel. The integrated data analysis software employs an open architecture that allows use of any DNA base-calling algorithm. DNA sequencing runs are completed in approx. 1 hr (approximately 500 bases), and instrument turnaround time between runs is less than 15 min. Overall, the instrument throughput is on the order of 720 templates/day, or 360,000 bases/day.

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Animals Electrophoresis, Capillary Humans Sequence Analysis, DNA Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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M Marsh, O Tu, V Dolnik, D Roach, N Solomon, K Bechtol, P Smietana, L Wang, X Li, P Cartwright, A Marks, D Barker, D Harris, J Bashkin. High-throughput DNA sequencing on a capillary array electrophoresis system.. https://pubmed.ncbi.nlm.nih.gov/9624574/

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