Intensity modulation of pseudocolor images.

L E Hinman; P J Sammak

doi:10.2144/98251bc01

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Intensity modulation of pseudocolor images.

L E Hinman·P J Sammak

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Abstract

Fura-2 is a popular real-time fluorescent indicator used to image intracellular, free calcium concentrations ([Ca2+]i) in living cells. One difficulty with fura-2-based digital imaging is how to best display the [Ca2+]i information that is acquired. Typically, only the [Ca2+]i as pseudocolor data are displayed, and the localization and intensity of the dye are not presented. This paper describes a technique that allows for the re-introduction of the intensity information. Recovery of the intensity information would de-emphasize regions with a poor signal-to-noise ratio and would more accurately report variations of sample morphology. A second advantage of this technique is that it requires no additional hardware since it is completely software based and can be run on all the major operating systems.

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Animals Calcium Color Color Perception Data Display Fluorescent Dyes Fura-2 Image Processing, Computer-Assisted Pulmonary Alveoli Rats Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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L E Hinman, P J Sammak. 1998. Intensity modulation of pseudocolor images.. https://doi.org/10.2144/98251bc01

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