A cosmid specific for sequences encoding a microtubule-associated protein, MAP1B, contains a polymorphic microsatellite and maps to bovine chromosome 20q14.

A Eggen; S Solinas-Toldo; R Fries

doi:10.1093/jhered/89.4.359

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A cosmid specific for sequences encoding a microtubule-associated protein, MAP1B, contains a polymorphic microsatellite and maps to bovine chromosome 20q14.

A Eggen·S Solinas-Toldo·R Fries

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Abstract

A clone for the bovine gene MAP1B was isolated from a bovine cosmid library with a probe obtained by cross-species PCR. The single positive cosmid clone was localized by FISH to chromosome region 20q14. Analysis of the cosmid revealed the presence of a microsatellite motif with a two-allele polymorphism detectable by PCR amplification of genomic DNA. However, the analysis of this polymorphism in a bovine family affected by SMA indicated absence of very close linkage of the phenotype for bovine SMA with MAP1B.

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Animals Base Sequence Cattle Chromosome Mapping Cloning, Molecular Cosmids DNA, Complementary Dinucleotide Repeats Female Male Microtubule-Associated Proteins Molecular Sequence Data Muscular Atrophy Pedigree Polymorphism, Genetic Journal Article Research Support, Non-U.S. Gov't

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A Eggen, S Solinas-Toldo, R Fries. A cosmid specific for sequences encoding a microtubule-associated protein, MAP1B, contains a polymorphic microsatellite and maps to bovine chromosome 20q14.. https://doi.org/10.1093/jhered%2F89.4.359

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