Non-invasive image acquisition and advanced processing in optical bioimaging.

D L Farkas; C Du; G W Fisher; C Lau; W Niu; E S Wachman; R M Levenson

doi:10.1016/s0895-6111(98)00011-1

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PubMed · 9719850

Non-invasive image acquisition and advanced processing in optical bioimaging.

D L Farkas·C Du·G W Fisher·C Lau·W Niu·E S Wachman·R M Levenson

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Abstract

Light is a most versatile tool for investigating biological systems and phenomena; the range, non-destructiveness, spatial discrimination and speed of optical imaging are all important for investigating structure and function at the cellular, tissue or even whole organism level. In live biological imaging, where the technological requirements are heightened, other features of light, such as coherence and wavelength, are used to generate the additional contrast and resolution needed. We report here recent improvements in our ability to image biological specimens optically, focusing on (a) spectral resolution and the related image processing issues, and (b) tomographic three-dimensional fluorescence imaging in vivo.

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Fluorescence Image Processing, Computer-Assisted Microscopy Optics and Photonics Spectrum Analysis Tomography Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

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D L Farkas, C Du, G W Fisher, C Lau, W Niu, E S Wachman, R M Levenson. Non-invasive image acquisition and advanced processing in optical bioimaging.. https://doi.org/10.1016/s0895-6111(98)00011-1

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