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Angiotensin-dependent gene expression in the developing rat kidney.

K F Hilgers·E S Pentz·R A Gomez
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Abstract

We aimed to identify genes involved in the growth effects of angiotensin II (Ang II) during kidney development. In rats treated from birth with the Ang II type-1 receptor blocker losartan, expression of transforming growth factor beta1 (TGF-beta1), platelet-derived growth factor B (PDGF-B), vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF), as measured by Northern blot, did not change significantly (N = 4 to 6 per group each). Differential display methods, used to identify genes with Ang II-dependent expression, produced mostly false positives. We identified one novel rat partial cDNA, termed AD.5, that is related to a human orphan receptor. AD.5 was expressed in a developmentally regulated pattern and may be involved in kidney development and/or the trophic actions of Ang II.

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Amino Acid SequenceAngiotensin IIAngiotensin Receptor AntagonistsAnimalsAnimals, NewbornAntihypertensive AgentsBlotting, NorthernEndothelial Growth FactorsEpidermal Growth FactorGene Expression Regulation, DevelopmentalGrowth SubstancesKidneyLosartanLymphokinesMolecular Sequence DataPolymerase Chain ReactionRNA, MessengerRatsRats, Sprague-DawleyReceptor, Platelet-Derived Growth Factor betaReceptors, Platelet-Derived Growth FactorTransforming Growth Factor betaVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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K F Hilgers, E S Pentz, R A Gomez. 1998. Angiotensin-dependent gene expression in the developing rat kidney.. https://doi.org/10.1046/j.1523-1755.1998.06729.x

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