HIV-1 reverse transcriptase is capable of elongating derivatives of sequence specific noncomplementary oligodeoxynucleotides.

I V Martyanov; O D Zakharova; E Sottofattori; G A Maksakova; D V Pyshnyi; M Mazzei; A Balbi; S Litvak; L Tarrago-Litvak; G A Nevinsky

doi:10.1002/iub.7510450502

PubMed · 9739449

HIV-1 reverse transcriptase is capable of elongating derivatives of sequence specific noncomplementary oligodeoxynucleotides.

I V Martyanov·O D Zakharova·E Sottofattori·G A Maksakova·D V Pyshnyi·M Mazzei·A Balbi·S Litvak·L Tarrago-Litvak·G A Nevinsky

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Abstract

We have carried out a comparison of KM and Vmax values for various primers in the polymerization reaction catalyzed by the HIV-1 RT. The affinity of RT for complementary d(pT)6 containing two different 5'-end pyranone derivatives was 2-3 orders of magnitude higher (KM = 3-15 nM) than that of d(pT)6 (KM = 12.6 mM). Oligodeoxynucleotides (ODNs) noncomplementary to poly(A) template were not elongated by RT. However, derivatives of d(CAGGTG) containing the 5'-terminal chromone and coumarin related groups were efficient primers showing KM (30-300 nM) and Vmax (75-93%) values comparable with that for d(pT)10 (800 nM; 100%). The [d(CAGGTG)]ddT ODN derivatives were effective inhibitors of RT. The primer function of derivatives of noncomplementary ODNs appears to be due to the additional interactions of their 5'-terminal groups with the enzyme tRNA-binding site.

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Binding Sites DNA Primers HIV Reverse Transcriptase Oligodeoxyribonucleotides Poly T RNA, Transfer Reverse Transcriptase Inhibitors Templates, Genetic Journal Article Research Support, Non-U.S. Gov't

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I V Martyanov, O D Zakharova, E Sottofattori, G A Maksakova, D V Pyshnyi, M Mazzei, A Balbi, S Litvak, L Tarrago-Litvak, G A Nevinsky. 1998. HIV-1 reverse transcriptase is capable of elongating derivatives of sequence specific noncomplementary oligodeoxynucleotides.. https://doi.org/10.1002/iub.7510450502

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