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PubMed · 994106

A highly efficient method for washing mammalian spermatozoa.

R A Harrison
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Abstract

A simple method has been developed for washing spermatozoa: the cell suspension is layered over a solution containing Ficoll and, after centrifugation, the original suspending medium remains as an undiluted layer above the Ficoll solution while the spermatozoa form a loose pellet at the bottom. Removal of the supernatant layers is easily and completely accomplished by aspiration. When ram spermatozoa were washed by this method, as much as 99-6% of the original medium could be removed during a single washing cycle, while up to 98% of the cells were recovered. Mechanical damage to the cells was minimal and repeatability was high.

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AnimalsCattleCell SurvivalCentrifugationFicollHorsesInulinMaleSheepSpecimen HandlingSperm MotilitySpermatozoaSwineJournal Article

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R A Harrison. 1976. A highly efficient method for washing mammalian spermatozoa.. https://doi.org/10.1530/jrf.0.0480347

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