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Velocity measurements of particulate neuroplasmic flow in organized mammalian CNS tissue cultures.

The movements of spherical particles in the range of 0.3 to 0.8 mum diameter within neurities of cultured embryonic mouse spinal cord fragments were observed and recorded by means of Nomarski optics and time-lapse photocinematography at high power. Particulate movements were measured by projecting the motion pictures onto a calibrated screen and recording the distances moved with time of linearly moving particles and making note of the direction (toward or away from the neuron soma) of movement. In all, 128 particles were measured in six cultures. These measurements were taken away from the neuron soma near the periphery of the neurites. Eight-three particles were noted to be moving toward the neuron at a mean velocity of 1.03 +/- 0.38 (SD) mum/sec while 45 anterograde moving particles were noted to move at 1.07 +/- 0.62 (SD) mum/sec. Statistical analysis of these veolcities revealed no significant difference between them. Particles which were elongated and probably represented mitochondria moved more sluggishly and could not be measured accurately by the techniques employed. It appeared the spherical particles moving in a retrograde direction originated at the neurite tip apparently by pinocytosis. There was a suggestion that anterograde flow and retrograde flow may have been affected unequally by factors which develop in the observation chamber over a period of 2 hr or more. The most likely factor responsible was probably hypoxia.

Animals↗

Bag model for DNA migration during pulsed-field electrophoresis.

A model for pulsed-field electrophoresis was developed by picturing large DNA as a deformable "bag" that (i) moves with limiting mobility in a continuous electric field, (ii) adopts an orientation aligned with the field direction, and (iii) reorients after a change in field direction in a size-dependent manner. The model correctly predicted the resolution of large DNA in a pulsed field including the surprising phenomena of mobility inversion, lateral band spreading, and improved resolution for obtuse angles. A simple parametrization agreed with observations of two completely different aspects of DNA behavior: bulk mobility as measured during gel electrophoresis and molecular reorientation as measured by linear dichroism. The model also provides quantitative guidelines for setting experimental parameters in pulsed-field electrophoresis experiments.

DNA↗

Is the social acceptability of psychiatric patients decreased by orofacial dyskinesia?

A group of psychiatric patients with and without orofacial dyskinesia (OFD) were filmed in pairs on videotape and a group of first-year social studies students were asked to select one candidate from each pair to fill a (simulated) job opening. A stationary picture (photograph) in which OFD was not visible and moving images (a video film) in which dyskinesia was visible were shown of each patient. The video effect, which was an indicator of the increase in popularity of a patient on the video film compared with the photograph, differed significantly between the OFD and control patients. Making the movement disability visible caused a relative decrease in the popularity of the OFD patients as opposed to an increase in the popularity of the control patients.

Adult↗

Occupation embedded in a real life: interweaving occupational science and occupational therapy. 1993 Eleanor Clarke Slagle Lecture.

This lecture presents an example of research in the genre of interpretive occupational science and demonstrates how occupational science can inform clinical practice. The innovative qualitative methodology used blended elements of the anthropological tradition of life history ethnography, ethnomethodology, the naturalistic methods used by Mattingly and Schön to study practice, and especially narrative analysis as described by Polkinghorne. The bulk of the paper is presented in the form of a narrative analysis that provides an account of a stroke survivor's personal struggle for recovery, a story that emerged from transcription, coding, and analysis of transcripts from approximately 20 hours of interview time. First, this narrative analysis provides an example of how the occupational science framework can evoke a particular kind of storytelling in which childhood occupation can be related to adult character. Storytelling of this kind is later shown to be therapeutic for the stroke survivor. Next, the narrative illustrates how rehabilitation can be experienced by the survivor as a rite of passage in which a person is moved to disability status and then abandoned. Finally, a picture is given of how occupational story making and occupational storytelling embedded in real life can nurture the human spirit to act and can become the core of clinical practice.

Persons with Disabilities↗

Cardiovascular health in Canadian women: the bigger picture revisited.

Cardiovascular disease (CVD) is the leading cause of death in Canadian women. Recent projections suggest that the number of cardiovascular-related deaths among women will continue to increase for at least another decade (Heart & Stroke Foundation of Canada, 2003). Nurses are in pivotal roles to facilitate the development of strategies to promote cardiovascular health and prevent CVD in this population. These strategies must move beyond the current focus on the individual, to encompass the bigger picture of population health promotion. This paper revisits the current state of knowledge of the population-based determinants of cardiovascular health in women, incorporates a Canadian perspective by including relevant epidemiological data, and recommends strategies that extend beyond the individual to the broader community, policy, health services and research domains.

Attitude to Health↗

Histological changes in Xenopus laevis Daudin specimens kept under dry conditions, then moved back to their natural aquatic environment. I. Pituitary, thyroid and testis.

The histofunctional picture of the hypophysis, thyroid and testis was studied in Xenopus laevis specimens 1) kept in their natural aquatic environment; 2) gradually exposed to dehydration conditions under which they were kept one week; and 3) returned from the dry environment to water for 24 hr or 7 days. Of particular interest are the changes displayed in the hypophysis by type II acidophils, i.e. presumably prolactin producing cells. In the pituitary of "dry" animals and of those 24 hr after their replacement in water these cells appear numerous, large-sized and heavily stained whereas they are small and slightly stainable in the pituitary of control animals or of "dry" ones 7 days after their replacement in water. On the basis of these results it is surmised that prolactin is continuously synthesized and released into the circulation in Xenopus specimens kept or replaced in water, thereby contributing to the animals adaptation to the aquatic environment, whereas in those kept under waterless condition prolactin synthesis is not discontinued, but its release into the bloodstream declines or is abolished. In the testis of the animals kept in dry conditions or 24 hr after replacement in water, the germ cells do not seem to have undergone substantial changes, while the sudanophilic material, which can be detected in interstitial tissues in the animals kept in water, is lacking. In all groups the thyroid histofunctional pattern suggests an intense activity, particularly in control animals or in "dry" specimens 7 days after replacement into water.

Animals↗

[The occlusal plane: an architectural myth or a proprioceptive myth?].

From two radiographic pictures of the head in profile, in meantime of few years, with the same person, the author proves that occlusal plane doesn't vary during growth in comparison with the pterygo-clivien compass (Stable zone leasing against pterygoïd process and superior clivus). The orientation of occlusal plane crossing by contact of the first molars and the middle of incisive intersection has an approximately analogous value. This value is equal to 110 degrees for the sample population, whatever the person's morphology and the lapsed time is. The occlusal plane moves parallel to himself and gets lower on a sagittal téléradiographic picture.

Adolescent↗

Effects on free recall of transforming visually and orally presented nouns into printed, picture, or imagery form.

Two experiments using male and female college students studied the effects on free recall of transforming auditory and verbal stimuli into various forms. Experiment 1 (n = 240) presented nouns either auditorily by a tape recorder or visually as printed words. The Ss either imagined, traced, or drew (used a pencil) the nouns in the forms of pictures and words, pictures only, or words only. Significantly more items were recalled as a result of Ss responding with both words and pictures, and pictures only, as opposed to words alone and a control groups (p less than .01). In Experiment 2 (n = 108), the nouns were presented auditorily by a tape recorder. The nouns were imagined in the form of either pictures or words, in either the size of one-half of normal, normal, or two times normal, and in either a stationary or moving position. Significantly more items were recalled as a result of imagining in the form of a picture during the presentation of the stimuli, and significantly more were also recalled as a result of imagining in the normal size as opposed to larger or smaller sizes (p less than .05). The results replicated previous findings and also contribute to the body of knowledge concerning effects of transformations on free recall.

Humans↗

When representations conflict with reality: the preschooler's problem with false beliefs and "false" photographs.

It has been argued that young preschoolers cannot correctly attribute a false belief to a deceived actor (Wimmer & Perner, 1983). Some researchers claim that the problem lies in the child's inadequate epistemology (Chandler & Boyes, 1982; Wellman, 1988); as such, it is specific to the child's theory of mind and no such problem should appear in reasoning about nonmental representations. This prediction is tested below in the "false photograph" task: here an actor takes a photograph of an object in location X; the object is then moved to location Y. Preschool subjects are asked: "In the picture, where is the object?" Results indicate that photographs are no easier to reason about than are beliefs. Manipulations to boost performance on the photograph task proved ineffective. Further, an explanation of the failure as a processing limitation having nothing to do with the representational nature of beliefs or photographs was ruled out. It is argued that young children's failure on the false belief task is not due to an inadequate epistemology (though they may have one) and is symptomatic of a larger problem with representations.

Child, Preschool↗

Mobile epididymis. A new clinicopathologic entity in genesis of male infertility and its treatment by epididymopexy.

Mobile epididymis plays an important role in the genesis of male infertility. It constituted 9 percent of a consecutive series of idiopathic infertility. Of 200 patients examined (100 fertile and 100 infertile), mobile epididymis was detected in 9 infertile patients. The clinical picture is characteristic. Epididymis is widely separated from testicle and moves freely from side to side. Its body and tail are ill formed and the epididymovasal angle is obtuse. Azoospermia was persistent in 3 patients and intermittent in 6 patients. Testicular biopsy showed tubular dilatation. Epididymopexy was performed in the 9 patients to fix the epididymis to testicle: 3 patients showed improvement in semen quality with two resultant pregnancies. Failures were due to advanced testicular damage. Infertility in mobile epididymis appears to result from obstruction of efferent ductules, testicular ischemia, and/or interference with sperm maturation, transport, or delivery.

Adult↗

Ball stud inspection system using machine vision.

In this paper, a vision-based inspection system that measures the dimensions of a ball stud is designed and implemented. The system acquires silhouetted images by backlighting and extracts the outlines of the nearly dichotomized images in subpixel accuracy. The sets of boundary data are modeled with reasonable geometric primitives and the parameters of the models are estimated in a manner that minimizes error. Jig-fixtures and servo systems for the inspection are also contrived. The system rotates an inspected object to recognize the objects in space not on a plane. The system moves the object vertically so that it may take several pictures of different parts of the object, resulting in improvement of measuring resolution. The performance of the system is evaluated by measurement of the dimensions of a standard ball, a standard cylinder, and a ball stud.

Calibration↗

[Preliminary results of treatment with vascular pedicled iliac bone chip in femur head necrosis].

For revascularisation of femoral head with osteonecrosis and pseudarthrosis of the femoral neck 68 femoral head were treated with a pedicled bonegraft. Therefore were taken the medial bonegraft (A. circumflexa ilium profunda) and the lateral bonegraft, based on the deep branches of the superior gluteal vessels. On the basis of postexamination it is reported about the first 35 patients, who were treated with a medial autological pedicled bonegraft. Prae- and postoperative each of them was clinical, radiological and angiological examinated. Because of this operation in 78.8% the clinical picture, existing about pain and the ability to walk and move, could be improved.

Adult↗

Evaluation of standardized methods for registration of time-dependent orthodontic tooth movements.

An in vitro study was conducted to compare reproducibility of repeated recordings obtained from devices constructed for standardized registrations. An existing radiographic method and a new photographic technique were employed. On five in vitro models, half of the maxilla from dry skulls, special designed splints with a Troll-biten film holder were constructed. A stiff metal bar attached to the splints and to either an X-ray apparatus or a standard photographic camera made it possible to obtain standardized registrations. For each model 10 radiographs and photographs were taken before and after the canine was moved. Subtracting the values from well defined points for these pictures, 10 pairs of coordinates were available for each of the models with both methods. The findings showed very similar reproducibility for the two methods with 95 per cent of the discrepancies within a magnitude of 0.12 mm for the radiographic method and 0.14 mm for the photographic method. When evaluating the canine movements, similar results were observed. No significant difference could be detected in the five in vitro models. In addition, the results showed that a splint can be used as a stable reference for registration of tooth movements. Because of ethical objections to unnecessary radiation, the photographic registration technique might substitute the radiographs in future investigations where standardized periodic records are necessary for the description of tooth movement.

Analysis of Variance↗

Clinical and outcome research in oncology. The need for integration.

Cancer is one of the main healthcare problems in Europe. Although significant progress has recently been made, long-term survival is still disappointing for most common solid tumours. The explosion of information has strengthened the need to create and sustain coordinated interaction between technology, biology, clinical research, clinical practice and health policy. A simple process based on automatic and passive translation from bench to clinical research and eventually to the bed side is usually assumed but cannot be taken for granted. A critical role might be played by Outcome Research (OR), defined as the discipline that describes, interprets, and predicts the impact of various influences, especially interventions, on final endpoints (from survival to satisfaction with care) that matter to decision makers (from patients to society at large), with special emphasis on the use of patient-reported outcomes (PRO). Recently, under pressure from several parts of society, the FDA, recognizing the need for faster drug approval, has modified existing regulations and created new rules to allow anti-cancer drugs to be approved more quickly and, in certain but quite common circumstances, single arm trials and surrogate endpoints to be used as measures of clinical benefit. In this context, the faster approval process may lead to drugs being marketed without there being a complete picture of how effective or safe they are. The FDA move to speed up drug approval, together with the use of not fully validated surrogate endpoints, give OR the unique opportunity to help understand the value of drugs that have received accelerated approval. Despite this opportunity, OR has yet to demonstrate its role in this specific setting and provide proof of the validity, reliability and added value of its primary endpoint measures when evaluated in a broader context. The implementation of lines of OR in the development and evaluation of anti-cancer drugs hinges upon the availability of specific knowledge, methods, instruments and resources and upon their appropriate integration in the mainstream of clinical research. In the USA specific interdisciplinary projects have been launched by the NCI. In Europe there is a lack of such initiatives. The correct placement of OR in the anti-cancer drug development process will guarantee the highest possible standard of validity and reliability of OR at European level and better integration of both translational and outcome research in the mainstream of clinical research into anti-cancer drugs, thus speeding up the introduction of the results of patient-oriented translational clinical research into clinical practice.

Antineoplastic Agents↗

RN-to-MSN students' attitudes toward women experiencing homelessness: A focus group study.

When health professionals, including RNs, have negative attitudes toward women experiencing homelessness, they create barriers to services. It is incumbent on nursing faculty to develop curricula that address homelessness and associated stereotypes, as well as to prepare students to provide safe and appropriate care to the homeless population. The aim of this qualitative study was to examine the attitudes of RN-to-MSN students toward mothers living with their children in a transitional shelter. A convenience sample of 10 students enrolled in a community health nursing course at a university in southern California participated in the study. Two focus groups were conducted: one before and one after a 15-week clinical experience. Data analysis revealed that during the clinical experience, students discovered that they, or perhaps an individual like them, could become homeless. Their attitudes and views changed to include a bigger picture of homelessness, described by public health nursing researchers as "moving upstream." This article suggests strategies for integrating clinical experiences with socioeconomically vulnerable individuals into undergraduate nursing curricula.

Attitude of Health Personnel↗

[Boundary conditions for excitation propagation equations which do not distort the picture of impulse movement].

New boundary conditions for the equations of excitation propagation are suggested. These conditions prescribe in the point in the end of the fiber the action potential which would be developed there, if half-infinite fiber would proceed further. The conditions suggested 1) in a number of cases allow to obtain an undistorted picture of excitation propagation, e.g. the picture when impulses fall out when a chain of impulses moves along the fiber; 2) save computer time and memory. Boundary conditions for syncitium and flat medium are considered.

Mathematics↗

The power of picture archiving and communication systems: strategic hospital considerations.

As healthcare information becomes more digitized, hospitals are adopting critical diagnostic tools to improve patient care decisions, while reducing errors and increasing overall efficiency. According to Radiology (2000), 75 percent of the imaging procedures performed in 1995 used devices that were not available in 1970. Today, imaging applications continue to move from research and development into common use each year With regard to digital imaging, Picture Archiving and Communication Systems (PACS) perhaps have changed radiology more than any other application in the past few decades. Once regarded as "nice-to-have-but-too-expensive" devices, PACS are rapidly moving into the mainstream of "must-have" solutions for quality-conscious and forward-thinking hospital CEOs. However, before widespread implementation of PACS can occur, several critical factors should be taken into consideration.

Competitive Bidding↗

Static depiction of movement.

A number of pictorial devices were compared in order to assess their relative effectiveness in depicting events. Effectiveness, as measured by a rating task, did not seem to be a function of the classification of a device as natural or metaphorical. Rather, it depended on whether a given device highlighted a distinctive aspect of a particular event (running, jumping, or moving), or simply modified the event. This observation was buttressed by the existence in a forced-choice task of a category boundary for depictions of running (pictures with a certain device were seen as running, those without it were not) but not for moving or jumping. Even preschool children displayed some understanding of metaphorical devices. They chose figures with those devices as running faster than the experimental standard. Results are discussed in the context of Kennedy's characterization of pictorial metaphors and Gibson's framework for understanding the relationship between pictorial and environmental information.

Adult↗