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Influence of flowable composite lining thickness on Class II composite restorations.

This in vitro study aimed to investigate the influence of flowable composite lining with different thicknesses on the marginal quality and internal porosity of Class II composite restorations. Thirty-two intact molars, each prepared with two box-only Class II cavities, were randomly divided into four groups: Group 1, P60 filling alone; Group 2, ultrathin flowable composite lining/co-cured with overlaying composite; Group 3, thin lining/pre-cured and Group 4, thick lining/pre-cured. The teeth were then thermocycled for 1500 cycles (between 5 degrees C and 60 degrees C) and dye immersed for 24 hours. Exterior surface replicas of these restorations were fabricated before and after thermocycling and examined by SEM to evaluate percentages of the five marginal patterns. Data was statistically evaluated using one-way ANOVA test. The teeth were subsequently sectioned longitudinally. The interface microleakage of cervical margin was measured as to the extent of dye penetration. Internal voids were separately recorded in the cervical interface and the cervical and occlusal halves of the restorations. Mann-Whitney test was applied to analyze the interface microleakage and internal voids. Results revealed that replicas of Group 4 presented the highest percentage of marginal openings both before and after thermocycling in SEM examination. Group 2 exhibited superior marginal quality in interface microleakage evaluation compared to the other groups, while Group 4 exhibited the worst. The pre-cured groups (Group 3 and 4) showed significant reduction in interface and cervical voids. Despite the reduction in interface voids, a thick lining may impair the marginal sealing, especially after thermocycling. It was concluded that a minimally thin flowable composite lining improved cavity adaptation and marginal sealing.

Analysis of Variance↗

Dentinal and pulpal uptake of mercury from lined and unlined amalgam restorations in minipigs.

The aim of the present investigation was to examine dentin and pulp in amalgam-restored teeth in minipigs with respect to presence of mercury, and to evaluate whether lining of restored cavities with a resin-modified glass ionomer cement had any effect on the penetration of mercury. Class I and V cavities were cut in 49 posterior and canine teeth of 4 minipigs. 35 cavities were restored with amalgam and 14 with composite material. 26 of the amalgam cavities were lined with a resin-modified glass ionomer cement. Following observation periods of 17-28 months, the pigs were killed and the jaws sectioned. After decalcification, the teeth were processed for light or electron microscopy examinations. Mercury was visualized by autometallography. Silver-enhanced mercury was found in all teeth with amalgam, whereas teeth with composites were devoid of mercury. Mercury could be traced in the odontoblast processes, in the body of odontoblasts, and on rare occasions in the nerve tissue of the pulp from lined and from unlined amalgam restorations. The present study thus demonstrates transport of mercury through dentinal tubules to the pulp, and that lining of cavities with a resin-modified glass ionomer cement does not prevent penetration of mercury.

Animals↗

Effect of a resin lining and rebonding on the marginal leakage of amalgam restorations.

This in vitro study compared microleakage in Class V amalgam restorations with three different lining agents and with no lining agent, with and without resin rebonding of the margins. Eight groups of ten molars were divided into four pairs of groups. Class V preparations were cut in the facial surfaces of each molar so that the occlusal margin was in enamel and the gingival margin in dentine. The preparations in one pair of groups received no lining agent; the preparations in the remaining three pairs of groups were lined with one of the following: Copalite, Universal Bond 3 (UB3) Primer and Adhesive, or UB3 Primer only. The enamel walls of the preparations receiving the UB3 Primer and Adhesive were etched prior to applying the lining material. Amalgam was hand condensed into each preparation. In one of each pair of groups, the margins of restorations were treated with 37% phosphoric acid gel, rinsed and dried, and UB3 Adhesive was applied over amalgam and tooth margins and polymerized (rebonded). Specimens were thermocycled, stained and sectioned. Microleakage was graded using a stereomicroscope. Mean microleakage scores for occlusal and gingival margins were calculated and analysed. At enamel margins, non-rebonded Copalite and all rebonded specimens showed less microleakage than the other non-rebonded groups. The group lined with UB3 Primer only and rebonded showed significantly less (P < 0.01) microleakage at enamel margins than all the other groups except the group lined with Copalite and rebonded. At cementum/dentine margins, restorations lined with UB3 Primer and rebonded showed significantly less microleakage than the other groups.

Acid Etching, Dental↗

Effect of bonding systems on post-operative sensitivity from posterior composites.

PURPOSE: To compare post-operative sensitivity from posterior composites lined with bonding systems that utilize either a self-etching primer or phosphoric acid conditioner. METHODS: Occlusal cavities on homologous contralateral posterior teeth in 28 selected subjects were lined with either One-Step Plus (a bonding system that utilizes phosphoric acid conditioner) or Clearfil SE Bond with a self-etching primer, and then restored with a hybrid resin composite. Post-operative sensitivity was assessed subjectively by asking the patient to classify pain from the restored tooth into none, mild or severe; and objectively by measuring the time it took for the patient to feel cold sensation when standardized ice stick was placed against the mid-buccal surface of the tooth. RESULTS: Subjective assessment showed that post-operative sensitivity was mild with either of the bonding systems; and marginal homogeneity test showed no statistically significant difference in the prevalence of post-operative sensitivity (P> 0.05), although there was a trend towards decreasing sensitivity with Clearfil SE Bond as post-operative period increased (P= 0.027). In the objective assessment, repeated measures of ANOVA showed that cold response measurements increased significantly with postoperative period only in the teeth lined with Clearfil SE Bond (P< 0.05), indicating decreasing post-operative sensitivity. However, paired t-test showed no significant difference between the cold response measurements obtained for the two bonding systems (P> 0.05).

Acid Etching, Dental↗

Bacteria beneath composite restorations--a culturing and histobacteriological study.

The occurrence, viability and identification of the microbial flora under composite fillings using an anaerobic technique were studied. Class V cavities were prepared on clinically healthy buccal surfaces of 7 contralateral pairs of premolars in children 11--15 years of age. After preparation, rubber dam was applied and one cavity in each pair of teeth was washed with water blasted dry with air and filled with Adaptic. The other cavity was washed with a cavity cleaner (Tubulicid) and a cavity liner (Tubulitec) was applicated prior to filling with Adaptic. The teeth were extracted after 4--6 weeks. Under anerobic conditions the tooth crown was split. From one half samples were taken from the pulpal wall under the filling and cultured on blood agar and in broth medium. The other half was examined with histobacteriological technique. No growth occurred in cultures from lined cavities but in 6 of the 7 unlined cavities. Full agreement was observed between the findings from the culturing and histobacteriological examinations concerning presence or absence of microorganisms at the pulpal wall in all 14 teeth. The flora was mixed. Gram-positive bacteria, mainly Streptococcus and Actinomyces, dominated over gram-negative bacteria including Veillonella, Fusobacterium, Campylobacter and Selenomonas. The composition of the flora was more similar to that observed in dental plaque than that found in carious dentin or in saliva in other studies.

Adolescent↗

Microleakage of Class II box-type composite restorations.

PURPOSE: To investigate whether in small box-type Class II preparations the use of glass ionomer cement, a dentin primer or a different type of conditioner had an effect on the microleakage compared to a more simple total-etch system using a phosphonated bonding agent. MATERIALS AND METHODS: In extracted premolars, 84 box-type Class II composite resin restorations with margins in enamel were made following a standardized protocol. A transparent matrix system was used and the composite resin was applied in two layers. The teeth were restored using a phosphonated total-etch bonding system as a control (Photo Bond). In the experimental groups a glass ionomer lining-cement (Vitrebond), a total-etch adhesive including a primer (Scotchbond Multi-Purpose) and a total-etch adhesive using a self-etching primer (Liner Bond 2) were used. The teeth were thermocycled, immersed in a dye solution and sectioned. The cervical and occlusal dye penetration was assessed. RESULTS: Preventing microleakage was not more effective in the experimental groups than in the control group (P < 0.05). Scotchbond MP groups using maleic acid showed statistically significant more microleakage than the control group (Photo Bond) at the cervical side. Liner Bond 2 and Scotchbond MP groups using maleic acid showed statistically significant more microleakage than the control group at the occlusal side. The use of a lining cement did not improve the quality of the restoration. From this study it was concluded that in this type of restoration, total-etch systems using a primer are not always an improvement.

Acid Etching, Dental↗

Microleakage in conventional and bonded amalgam restorations: influence of cavity volume.

This study verified the relationship between the volume and microleakage of conventional and bonded amalgam restorations. Also, the microleakage influence of intermediate materials, substrates and the direction of sectioning was investigated. Fifty-six bovine incisors were selected. Standard Class V cavities were prepared in buccal and lingual surfaces. For each tooth, two cavity sizes were prepared, corresponding to two cavity volumes: one larger (A) and the other smaller (B). The cervical wall was located in cementum/dentin and the incisal wall in enamel. The teeth were distributed in four groups (n=28) according to the intermediate material employed (glass-ionomer cement, resin cement, adhesive system and copal varnish-control). The materials were applied following manufacturers' directions. After restoration, the teeth were submitted to thermal cycling. They were then immersed in a dye solution and sectioned in two directions inciso-cervical (IC) and mesio-distal (MD) sections to evaluate the microleakage. Data were subjected to non-parametric statistical analysis (Wilcoxon's paired test and Kruskal-Wallis test). No significant difference was found between the two cavity sizes. Leakage in enamel was statistically lower than in the cementum/dentin interface (p < 0.05). In some situations, glass-ionomer or resin cement lined amalgam restorations presented less dye leakage than copal varnish lined restorations (p < 0.05). No significant difference was observed in microleakage between IC or MD sectioning. Within the limitations of this study, it was concluded that cavity size and direction of section were not significant factors for microleakage, while substrate and intermediate materials had a significant effect on the sealing ability in amalgam restorations.

Animals↗

Sealing effectiveness of light-cured glass ionomer cement liners.

Glass ionomer materials have been used as liners or bases beneath restorations, but light-cured varieties have recently been introduced. Microleakage at the restoration/tooth interface in cervical cavities was used to test the adhesion to dentin of two new light-cured glass ionomer cement lining materials, XR-Ionomer and Fuji Lining LC. Wedge-shaped cervical cavities were prepared on extracted teeth with a gingival cavosurface margin involving dentin, and the specimens were assigned randomly to three groups. The experimental groups were restored with two light-cured glass ionomer cements, the controls were restored with a chemically cured glass ionomer cement; and marginal leakage was assessed by dye penetration. The two light-cured glass ionomer cements displayed some adhesion to dentin in class V test cavities, but there was no significant difference between the two materials.

Dental Bonding↗

Monkey pulp reactions to restorative materials.

Deep buccal cavities in 99 teeth in eight young monkeys were filled with the following combination of materials: a light-cured microfilled composite with or without a base, a chemically cured composite with a base, a silicate cement, and a zinc oxide-eugenol cement. The acid etch technique and intermediate layer of resin was used in the composite group. Pulp reactions and presence and location of bacteria were studied after 8 days and after 90 days. In the short observation period the inflammatory reactions were more pronounced when unlined composite fillings were evaluated compared with silicate cement fillings and with lined fillings. Bacteria were seen in all unlined cavities and a significant association between presence of bacteria and moderate to severe inflammatory responses was found. The most severe inflammatory reactions were seen when bacteria were found in the dentinal tubules. After 90 days slight inflammatory changes prevailed in all groups. A significant correlation between bacteria and inflammatory reactions could still be observed.

Acrylic Resins↗

Current clinical concepts for adhesive cementation of tooth-colored posterior restorations.

The adhesive luting of tooth-colored posterior restorations has long been considered an unreliable clinical procedure. The physical and clinical properties of former base lining materials, the absence of an effective peripheral biological seal during provisionalization, and the use of less than optimal adhesive systems and luting cements have prevented the achievement of satisfactory clinical results. This article describes effective procedures for adhesive cementation based on the application of distinctive layers and the use of densely filled, viscous luting materials.

Cementation↗

[Effect of various cavity design, lining and restorative materials on the fracture resistance of teeth].

OBJECTIVE: To evaluate the influence of various cavity designs, lining and restorative materials on fracture resistance of teeth in vitro. METHODS: Factorial design with three factors and various levels was used. 36 extracted human maxillary premalors were prepared for occlusal cavity, MO cavity and MOD cavity. Various base material and restorations were used. All teeth were thermocycled and mounted for testing and then were loaded until fracture. The results were analyzed by a three-way ANOVA. Fracture patterns were observed at the same time. RESULTS: There was significant difference in fracture resistance among various cavity designs and restorations. The fracture patterns were different. CONCLUSIONS: The different classes of cavities and various restorations have significantly influence upon fracture resistance of teeth. There are interactions between cavities and restorations.

Dental Cavity Lining↗

Effect of bonded amalgam on the fracture resistance of teeth.

The purpose of this study was to determine whether amalgam bonded to tooth structure with an adhesive resin cement can increase the fracture resistance of restored teeth. Extracted paired upper premolars were prepared for G.V. Black type mesioocclusodistal amalgam restorations. In one tooth of each pair (the experimental group), the enamel walls were etched with phosphoric acid and were painted with an adhesive resin (Panavia), and amalgam was condensed and carved. For the other tooth in each pair (the control group), amalgam was placed in the same manner but was not etched and lined with resin. The teeth were thermocycled and mounted for testing and then were loaded until fracture. A significant difference (p less than 0.05, the paired Student's t-test) was found in the force needed to fracture the bonded amalgam group (70.5 +/- 21.6 kg) compared with that needed to fracture the conventional amalgam group (60.3 +/- 16.8 kg). SEM examination of fractures at the interface occurred predominantly within the resin.

Acid Etching, Dental↗

Fluoride uptake around cavity walls; two-dimensional mapping by electron probe microanalysis.

This study reports fluoride uptake around the cavity wall of teeth by two-dimensional mapping. Fluoride concentration was measured using the wavelength dispersive x-ray analysis (WDX) method. The buccal cavity wall of a human tooth was coated five times with 2% sodium fluoride solution at three-day intervals for 12 days, and then immersed in a normal saline solution at 37 degrees C. After one month, the tooth was bisected longitudinally through the center of the cavity surface perpendicular to the axial wall. On the polished surface of the cut tooth, the fluoride concentration was measured. Fluoride distribution maps around the cavity wall were drawn using a bundle of the observed analytical lines. Fluoride uptake from fluoride-releasing materials (conventional glass-ionomer cement, light-cured glass-ionomer cement, light-cured composite resin, light-cured bonding agent) around the cavity wall was investigated using the same method. The maps showed higher fluoride uptake in dentin than in enamel and a strong location dependence of fluoride uptake in a tooth, especially in the dentin. Fluoride uptake from the resin was greater than that from the cement. It was summarized from these results that a two-dimensional map of fluoride uptake can provide valuable information on the cariostatic properties of fluoride-releasing materials.

Acid Etching, Dental↗

Dentin-polymer bond in resin fillings tested in vitro by thermo- and load-cycling.

The effect of stress applied by thermo- and load-cycling to the bond between composite- and bonding-agent-treated dentin was tested. Cylindrical cavities in extracted human teeth, approximately one-half of the margins in dentin, were etched and treated with two bonding agents - Gluma and Clearfil Bond - before being filled with Silux composite. Forty specimens, some provided with cavity floor lining, were cycled several hundred times between 15 degrees and 50 degrees C, either immediately after light-curing or upon 24 hr of water storage. In all cases, microscopic inspection revealed unchanged adaptation at the dentin margins. Teeth with Class 3 and Class 5 cavities, ten of each and with the gingival margins entirely in dentin, were pre-treated and filled as above. The teeth were covered with a dye during loading and unloading in a manner simulating biting and chewing. Inspection of the cavity margins revealed absence of percolation at the dentin margins. It is concluded that effective bonding agents are necessary to prevent contraction gaps in resin-filled cavities where the margins are partly or entirely located in dentin.

Bisphenol A-Glycidyl Methacrylate↗

[Effect of remaining dentine thickness beneath deep cavities on the rate of reparative dentine formation--experimental study].

The aim of this study was to investigate the effect of remaining dentine thickness beneath deep cavities on the rate of reparative dentine formation throughout 119 days and to establish the limit value of the remaining dentine thickness that enables greatest amount of tertiary dentine in dogs' teeth. Deep buccal cavities were prepared in all canines and molars of three dogs, lined with four different lining materials for indirect pulp capping and filled with silver amalgam. Tetracycline labelling technique was used to demark the layers of reparative dentine produced during the experimental period of 17 weeks. After sacrificing and isolation the teeth from the jaws slices (1 mm thick) were cut off through the middle of the cavity, perpendicular to the cavity floor and mesio-distal tooth axis. Sections were ground to 100 microns thick; the measuring of the remaining and reparative dentine thickness was carried out with Fluoval, Carl-Zeiss, Jena, DDR fluorescent microscope. The results showed: 1. Cavity depth had a strong effect on the quantity of newly formed tertiary dentine; 2. The greatest rate of reparative dentine (348 microns) was produced beneath the cavities with remaining dentine thickness ranging 501-700 microns; 3. The least quantities of tertiary dentine (219 and 227 microns) were produced beneath the deepest cavities, with remaining dentine thickness less than 500 microns, as well as beneath shallower cavities with remaining dentine thickness of over 900 microns.

Animals↗

A new method for sealing composite resin contraction gaps in lined cavities.

Composite resin restorations are often applied in cavities where cervical margins are left unetched, resulting in poorer adaptation in this area. Recent experiments have indicated the possibility of impregnating with resin the gap caused by polymerization contraction. Cavities were prepared in vitro and in vivo. A thin liner was applied inside and outside the cavity. Except for the cervical wall, enamel margins were bevelled and acid-etched, and the cavities restored with Concise Enamel Bond and Composite. In another series, cavities with unetched enamel were restored. Since the liner was placed outside the cavity margins, excess material could be removed without blockage of the air-filled gap. Enamel Bond with a fluorescent additive was applied to various margins. Ground sections were prepared, and the penetration of this resin into the gap was examined with a microscope. In most teeth, the resin penetrated from 0.5 to 2 mm from the cervical margins. Similar penetration was seen from unetched margins, except for the occlusal one. No penetration was seen at acid-etched margins. By impregnating gaps with low-viscous resin after the filling has cured, one can obtain an improved seal at unetched margins.

Acid Etching, Dental↗

Internal adaptation and overhang formation of direct Class II resin composite restorations.

The aim of the present in vitro study was to evaluate different restorative concepts for posterior resin composite fillings in terms of internal adaptation and overhang formation. Eighty standard occluso-distal cavities with and without a 1.5-mm bevel were restored in a phantom head using Syntac Classic and Tetric Ceram with and without Tetric Flow as thin lining or Solid Bond and Solitaire with and without FlowLine. The restorations were finished intraorally and afterwards subjected to thermal loading (1150 x +5 degrees C/+55 degrees C) for 24 h. The proximal margins of the original specimens were analyzed for overhangs under a stereo light microscope (100-fold magnification) before and after intraoral control with loupes, including additional polish. Afterwards the teeth were cut longitudinally, replicated, and their internal integrity analyzed under a SEM (200-fold magnification). The combination of flowable and viscous composites resulted in enhanced internal adaptation for both adhesive systems. However, Syntac Classic exhibited superior adaptation characteristics compared with Solid Bond. In terms of overhang formation, the use of flowable materials always led to higher percentages of marginal overhangs in beveled cavities. Higher viscous materials alone resulted in higher percentages of underfilled margins of beveled than box-shaped cavities. It was clear that the use of magnifying glasses during finishing was beneficial for reducing marginal overhangs up to 40%.

Composite Resins↗

Microleakage and retention of bonded amalgam restorations.

PURPOSE: To compare the microleakage and bond strength exhibited by two light-cured, filled dentin bonding agents, a resin cement, and cavity varnish. MATERIALS AND METHODS: The four test groups of lining agents for amalgam restorations included (LF) Light-cured, Filled resin (Clearfil Liner Bond 2); (LCF) light- and chemical-cured, Filled resin (Clearfil Liner Bond + Protect Liner); (RC) Resin Cement (Panavia 21); and (V) Varnish (Copalite). For each group, 20 Class V cavity preparations were cut in human extracted molars. Tytin amalgam was condensed into the preparation. After storage for at least 24 hrs in distilled water at 37 degrees C, all restorations were subjected to 2500 thermal cycles (8 degrees C to 48 degrees C). After 1 wk, specimens were tested. For the leakage tests, teeth were immersed in 45Ca, sectioned, and radiographs of sections were evaluated and then evaluated by Ridit analysis. Retention samples were tested to failure in tension using an Instron machine and peak load (kg) was recorded. RESULTS: Compared to varnish, the two dentin bonding agents (LF and LCF) significantly decreased microleakage at the cementum margin but not at the enamel margin. The two dentin bonding agents also exhibited significantly greater retentive strength than either the resin cement or the varnish.

Calcium Radioisotopes↗