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DANCE, a novel secreted RGD protein expressed in developing, atherosclerotic, and balloon-injured arteries.

We have identified and characterized mouse, rat, and human cDNAs that encode a novel secreted protein of 448 amino acids named DANCE (developmental arteries and neural crest epidermal growth factor (EGF)-like). DANCE contains six calcium-binding EGF-like domains, one of which includes an RGD motif. Overexpression studies of recombinant DANCE protein document that DANCE is a secreted 66-kDa protein. DANCE and recently described protein S1-5 comprise a new EGF-like protein family. The human DANCE gene was mapped at chromosome 14q32.1. DANCE mRNA is mainly expressed in heart, ovary, and colon in adult human tissues. Expression profile analysis by in situ hybridization revealed prominent DANCE expression in developing arteries. DANCE is also expressed in neural crest cell derivatives, endocardial cushion tissue, and several other mesenchymal tissues. In adult vessels, DANCE expression is largely diminished but is reinduced in balloon-injured vessels and atherosclerotic lesions, notably in intimal vascular smooth muscle cells and endothelial cells that lose their ability to proliferate in late stage of injury. DANCE protein was shown to promote adhesion of endothelial cells through interaction of integrins and the RGD motif of DANCE. DANCE is thus a novel vascular ligand for integrin receptors and may play a role in vascular development and remodeling.

Amino Acid Sequence↗

The tremble dance of honey bees can be caused by hive-external foraging experience.

The tremble dance of honey bee nectar foragers is part of the communication system that regulates a colony's foraging efficiency. A forager that returns to the hive with nectar, but then experiences a long unloading delay because she has difficulty finding a nectar receiver bee, will perform a tremble dance to recruit additional nectar receiver bees. A forager that experiences a short unloading delay will perform a waggle dance to recruit more nectar foragers. A long unloading delay was until now the only known cause of tremble dancing. However, several studies suggested that factors at the food source may also cause tremble dancing. Here I test whether one of these factors, crowding of nectar foragers at the food source, stimulates tremble dancing because it causes long unloading delays. To do so, I increased the density of nectar foragers at a food source by suddenly reducing the size of an artificial feeder, and recorded the unloading delay experienced by each forager, as well as the dance she performed, if any. A forager's unloading delay was measured as the time interval between entering the hive and either (1) the first unloading contact with a nectar receiver bee, or (2) the start of the first dance, if dancing began before the first unloading contact. I also recorded the unloading delays and dances of nectar foragers that returned from natural food sources. The results show that crowding of nectar foragers at the food source increases the probability of tremble dancing, but does not cause long unloading delays, and that tremble dancers that foraged at natural food sources also often have short unloading delays. When the cause of the tremble dance is not a low supply of nectar receiver bees, the tremble dance may have a function in addition to the recruitment of nectar receiver bees.

Animal Communication↗

Energy cost of square dancing.

This experiment was concerned with determining the energy cost of two popular Western square dancing routines: the "Mish-Mash," which is a relatively fast-moving dance with quick movements, and the "Singing" dance, which is a slower and more deliberate type of dance. The subjects were four middle-aged couples, veteran members of a local square dancing club. Sitting and standing pulmonary ventilations were determined through the use of the Tissot gasometer. Kofrańyi-Michaelis respirometers were employed for the dance routine ventilations. These apparatus were fitted with a Monoghan neoprene cushion plastic mask. Gas samples were collected in polyethylene metallized bags and analyzed for O2 and CO2 content. The net energy cost for the two dances was appropriately summarized. The results indicated that for the males the net average energy cost of the "Mish-Mash" dance was 0.085 and 0.077 kcal/min per kg for the "Singing" dance. For the females, the cost was 0.088 and 0.084 kcal/min per kg, respectively. A net average cost of these two dances yielded a caloric expenditure of 5.7 kcal/min for a 70-kg male and 5.2 kcal/min for a 60-kg female. It was indicated that during the course of a typical square dance evening, a 70-kg man would expend some 425 kcal. while a 60-kg female would burn some 390 kcal. The energy cost of the dances studied were determined to be within the permissible work load of a functional class 1 patient with diseases of the heart as determined by the American Heart Association.

Adult↗

A comparison of the relation between oxygen uptake and heart rate during different styles of aerobic dance and a traditional step test in women.

The oxygen uptake and heart rate in various styles of dance and in a graded step test have been compared in ten healthy women aged [mean (SD)] 34 (5) years. Dance was choreographed into progressively more energetic sequences typical of community classes, and videotaped. Oxygen uptake was assessed using a respirometer carried in a back-pack. Each of the two tests (dance and step) took 15-20 min and measurements were made in randomised balanced order on the same day. The mean oxygen costs of dance ranged from 1.29 l.min-1 for low impact style to 1.83 l.min-1 for high impact style with arm work; mean heart rates were 135 and 174 beats.min-1 respectively. Low impact dance raised heart rates above 60% of predicted maximum and so would provide training; during high impact dance recorded heart rates sometimes exceeded recommended safe limits. The addition of arm work significantly increased heart rates in both high and low impact dance but when oxygen pulses for each style of dance were compared no significant differences attributable to arm work were found. Moreover calculated differences between oxygen uptakes in stepping and dance at the same heart rates (those recorded during dance) were not significant for any of the four styles. Analysis of variance confirmed that neither arm work nor impact contributed significantly to the differences, so there was no evidence that these forms of dance change the normal relation between heart rate and oxygen uptake found in dynamic activities with large muscle groups such as stepping.

Adult↗

Motor abilities in dance structure performance in female students.

The aim of the study was to analyze the relation between motor abilities and performance in folk dances originating from the island of Hvar and modern social dances. Two groups of variables were used in a sample of 78 female students of the Teacher Training College from Split: 7 motor variables as a battery of predictors, and performance evaluation of 4 dances (2 folk dances, i.e. ciciliona and pasavijen, and 2 social dances, i.e. cha-cha-cha and rock-'n'-roll) as criterion variables. Canonical correlation analysis between the groups of variables yielded two canonical correlations of 0.94 and 0.73, with a level of significance of p<0.001. The first canonical correlation was based on marked determination of coordination and ciciliona dance, and the second one on explosive strength of the running type with below-average coordination and the cha-cha-cha, rock-'n'-roll and pasavijen dances. Regression analysis indicated the battery of motor variables used to be a good predictor of performance in all study dances, with multiple correlation of 0.93 in ciciliona, 0.84 in pasavijen, 0.75 in rock-'n'-roll and 0.73 in cha-cha-cha. In ciciliona and pasavijen, the latent dance structure is predominantly explained by coordination, in rock-'n'-roll by explosive strength, and in cha-cha-cha by explosive strength and speed. Discriminative analysis revealed the general dance performance to mostly depend on coordination, then on explosive strength, and to a lesser extent on speed (movement frequency). Dance is an irreplaceable educational tool in kinesiologic education of female students, among others for its considerable contribution to the development and maintenance of basic motor abilities.

Adult↗

The effects of dance team participation on female adolescent physical fitness and self-concept.

The purpose of this study was to determine to what extent female physical fitness and self-concept are affected by dance team participation in high school. Eight dancers were tested once prior to and once four months after dance team participation. Eight participants from physical education classes, matched for age, weight, height, grade, and race, were tested once at the same time as the second dance team test. Physiological tests were maximum oxygen uptake, sit-and-reach, one-repetition maximum bench press, skinfolds, and hydrostatic weighing. The self-concept tests were Coopersmith Self-Esteem Inventory, Tennessee Self-Concept Scale, and Body Cathexis Scale. Dependent one-tailed t tests were run to determine differences between dance team pre- and posttests and control and dance team posttests. Dance subjects increased maximum oxygen uptake and one-repetition maximum bench press in addition to improving their body composition as evidenced by a significant decrease in total skinfolds and a near significant decrease in percent body fat. The dance team had a significantly higher maximum oxygen uptake than did controls. No other significant differences were seen between groups. Dance team participants significantly improved physical self and social self on the Tennessee Self-Concept Scale. No other significant differences were seen. A factor that may have affected the self-concept results in this study was low dance team status, due to a combination of unsuccessful previous dance teams and a losing football season. Within the limitations of this study, these results indicate that physical fitness is improved as a result of dance team participation; however, self-concept seems to be affected only minimally, if at all, as compared with participation in physical education classes.

Adolescent↗

A comparison of the dance language in Apis mellifera carnica and Apis florea reveals striking similarities.

Honeybees have a dance language by which successful foragers inform nestmates about attractive food patches. The classical concept of dialects in the dance language of honeybees points to two differences in the dances by different species and races, firstly in the flight distance at which the dancers start performing waggle dances instead of round dances, and secondly in the circuit duration of the waggle dance performed for a given flight distance. However, recent findings have indicated that the dance language is influenced and affected by a number of parameters, both genetic and environmental. The current study was carried out to see whether the distance at which dancers change from round dances to waggle dances is statistically different in two different species, Apis mellifera carnica and A. florea and to develop a set of definitions for such comparative studies. Results show that the two species do not differ in the relative proportion of waggle dances and round dances performed at a given distance. Thus, this study points to the need of addressing the dialect question again.

Animal Communication↗

The interplay between dancing and trophallactic behavior in the honey bee Apis mellifera.

The interplay between the recruitment dance and food-giving trophallactic contacts of returning Apis meellifera foragers was analyzed. Dancing and trophallactic events were recorded for bees returning from a rate feeder that provided 50% weight on weight sucrose solution at a constant flow rate of 5 microl min(-1). Bees that had danced immediately before their trophallactic contact had more recipients per trophallaxis compared with bees that did not dance before. Thus, besides information coded in dancing behavior, dance maneuvers could serve as a stimulus to increase attention of bees located on the dance floor to receive nectar. In addition, the number of bees receiving food during a trophallaxis showed a positive correlation with the probability of dancing immediately after contacting. The time from arrival at the hive to when the first or the subsequent contacts took place presented no correlation with the probability of dancing after trophallaxis. Also, the duration of a trophallaxis was positively correlated with the number of recipients per trophallaxis. These results suggest that returning foragers could receive information during a trophallactic contact with their hive mates that modify thresholds for dancing. Dance maneuvers and trophallactic contacts performed by foraging bees seem to be "mutually" affected.

Animals↗

Encoding spatial information in the waggle dance.

Apis mellifera bees execute waggle dances to recruit other bees to desirable food sources. Several components of the waggle dance are correlated with the direction of and the distance to food. Moreover, recruits use the spatial information encoded in the dance to locate the signalled food. However, although dance communication has been studied extensively, little is known about how the dancers combine the compass (direction) and the odometric (distance) information they acquire during the foraging flight. In the present study, we analysed the encoding of spatial information in the waggle dance by manipulating the navigational information provided to dancing bees. To this end, we took advantage of the bees' visually driven odometer. We found that the waggle dance basically encodes information on the distance gauged during the outbound (hive-to-food) flight. However, it does not necessarily refer to a global vector based on path integration of the outbound flight. Whenever the direction connecting the subjective food location and the hive does not match the direction of the global vector, dancers refer to a direction close to that of the shortcut connecting the actual food location and the hive. Moreover, in our experiments, this direction was close to that of the inbound (food-to-hive) flight, indicating that landmark-based information is computed during the inbound flight and that it may strongly affect the encoding of directional information in the waggle dance. Moreover, we found that the bees' experience of the terrain modulates the encoding of spatial information in the waggle dance, suggesting that interactions between path integration and visual landmarks are computed in the context of dance communication.

Animal Communication↗

Honeybee colonies achieve fitness through dancing.

The honeybee dance language, in which foragers perform dances containing information about the distance and direction to food sources, is the quintessential example of symbolic communication in non-primates. The dance language has been the subject of controversy, and of extensive research into the mechanisms of acquiring, decoding and evaluating the information in the dance. The dance language has been hypothesized, but not shown, to increase colony food collection. Here we show that colonies with disoriented dances (lacking direction information) recruit less effectively to syrup feeders than do colonies with oriented dances. For colonies foraging at natural sources, the direction information sometimes increases food collected, but at other times it makes no difference. The food-location information in the dance is presumably important when food sources are hard to find, variable in richness and ephemeral. Recruitment based simply on arousal of foragers and communication of floral odour, as occurs in honeybees, bumble bees and some stingless bees, can be equally effective under other circumstances. Clarifying the condition-dependent payoffs of the dance language provides new insight into its function in honeybee ecology.

Animal Communication↗

Dance reveals symmetry especially in young men.

Dance is believed to be important in the courtship of a variety of species, including humans, but nothing is known about what dance reveals about the underlying phenotypic--or genotypic--quality of the dancer. One measure of quality in evolutionary studies is the degree of bodily symmetry (fluctuating asymmetry, FA), because it measures developmental stability. Does dance quality reveal FA to the observer and is the effect stronger for male dancers than female? To answer these questions, we chose a population that has been measured twice for FA since 1996 (ref. 9) in a society (Jamaican) in which dancing is important in the lives of both sexes. Motion-capture cameras created controlled stimuli (in the form of videos) that isolated dance movements from all other aspects of visual appearance (including FA), and the same population evaluated these videos for dancing ability. Here we report that there are strong positive associations between symmetry and dancing ability, and these associations were stronger in men than in women. In addition, women rate dances by symmetrical men relatively more positively than do men, and more-symmetrical men value symmetry in women dancers more than do less-symmetrical men. In summary, dance in Jamaica seems to show evidence of sexual selection and to reveal important information about the dancer.

Adolescent↗

Heart rate and estimated energy expenditure during ballroom dancing.

Ten competitive ballroom dance couples performed simulated competitive sequences of Modern and Latin American dance. Heart rate was telemetered during the dance sequences and related to direct measures of oxygen uptake and heart rate obtained while walking on a treadmill. Linear regression was employed to estimate gross and net energy expenditures of the dance sequences. A multivariate analysis of variance with repeated measures on the dance factor was applied to the data to test for interaction and main effects on the sex and dance factors. Overall mean heart rate values for the Modern dance sequence were 170 beats.min-1 and 173 beats.min-1 for males and females respectively. During the Latin American sequence mean overall heart rate for males was 168 beats.min-1 and 177 beats.min-1 for females. Predicted mean gross values of oxygen consumption for the males were 42.8 +/- 5.7 ml.kg-1 min-1 and 42.8 +/- 6.9 ml.kg-1 min-1 for the Modern and Latin American sequences respectively. Corresponding gross estimates of oxygen consumption for the females were 34.7 +/- 3.8 ml.kg-1 min-1 and 36.1 +/- 4.1 ml.kg-1 min-1. Males were estimated to expand 54.1 +/- 8.1 kJ.min-1 of energy during the Modern sequence and 54.0 +/- 9.6 kJ.min-1 during the Latin American sequence, while predicted energy expenditure for females was 34.7 +/- 3.8 kJ.min-1 and 36.1 +/- 4.1 kJ.min-1 for Modern and Latin American dance respectively. The results suggested that both males and females were dancing at greater than 80% of their maximum oxygen consumption. A significant difference between males and females was observed for predicted gross and net values of oxygen consumption (in L.min-1 and ml.kg-1 min-1).

Adolescent↗

Dance-related injury.

Although dance medicine has derived extensive knowledge from sports medicine, some aspects covered in the practice of dance medicine are unique to this field. Acute and overuse injuries must be analyzed within the scope of associated mechanisms of injury, mainly related to the practice of specific dance techniques. Even though most available medical literature concerning dance medicine is specific to ballet-related conditions, many of the concepts covered here and in other articles can be helpful in the treatment and diagnosis of participants in other dance disciplines. Continued research is expanding the knowledge on injury patterns of different dance disciplines. It is the experience of dance practitioners that dancers are quite in touch with their bodies; thus, when their ailments are systematically analyzed, and underlying cause can usually be identified. In this sense, it is evident that the principles of dance medicine and rehabilitation allow the practitioner to arrive at a diagnosis and treat the underlying causes to prevent reinjury, ameliorate sequelae from injury, and minimize residual deficits after injury.

Ankle Injuries↗

The effect of pre-season dance training on physical indices and back pain in elite cross-country skiers: a prospective controlled intervention study.

OBJECTIVE: To evaluate the effect of pre-season dance training on back pain, joint mobility, and muscle flexibility, and on speed and agility in elite cross-country skiers. METHODS: 26 skiers participated (mean (SD) age, 19 (3.9) years). An intervention group (n = 16) had 12 weeks of dance training; a control group (n = 10) did not dance; otherwise both groups followed a similar pre-season physical training programme. Joint mobility and muscle flexibility of the spine, hip, and ankle were measured. Two sports related functional tests (slalom and hurdle) were also done. All measurements/tests were carried out before and after the dancing period. RESULTS: Four (of six) subjects from the intervention group who initially complained of ski related back pain did not report back pain after the dance training; the three subjects with back pain from the control group were unchanged. At study onset the intervention group had a slightly impaired range of motion in the spine compared with the control group. After dance training, there was a better relation between kyphosis of the thoracic spine and lordosis of the lumbar spine, and a 7.1 degrees increase in hip flexion with the knee extended (p = 0.02). In the control group hip extension decreased by 0.08 m on average (p = 0.01). No positive effects of dance training on sports related functional tests were observed. CONCLUSIONS: Preseason dance training improved the range of hip motion and joint mobility and the flexibility of the spine. These improvements might explain the reduction in ski related back pain in the intervention group.

Adolescent↗

Effects of Hatha yoga and African dance on perceived stress, affect, and salivary cortisol.

BACKGROUND: Dance and yoga have been shown to produce improvements in psychological well-being. PURPOSE: The aim of this study was to examine some of the psychological and neuroendocrine response to these activities. METHODS: Sixty-nine healthy college students participated in one of three 90-min classes: African dance (n = 21), Hatha yoga (n= 18), or a biology lecture as a control session (n = 30). Before and after each condition participants completed the Perceived Stress Scale (PSS), completed the Positive Affect and Negative Affect Schedule, and provided a saliva sample for cortisol. RESULTS: There were significant reductions in PSS and negative affect (ps < .0001) and Time x Treatment interactions (ps < .0001) such that African dance and Hatha yoga showed significant declines, whereas there was no significant change in biology lecture. There was no significant main effect for positive affect (p = .53), however there was a significant interaction effect (p < .001) such that positive affect increased in African dance, decreased in biology lecture, and did not change significantly in Hatha yoga. There was a significant main effect for salivary cortisol (p < .05) and a significant interaction effect (p < .0001) such that cortisol increased in African dance, decreased in Hatha yoga, and did not change in biology. Changes in cortisol were not significantly related to changes in psychological variables across treatments. There was 1 significant interaction effect (p = .04) such that change in positive affect and change in cortisol were negatively correlated in Hatha yoga but positively correlated in Africa dance and biology. CONCLUSIONS: Both African dance and Hatha yoga reduced perceived stress and negative affect. Cortisol increased in African dance and decreased in Hatha yoga. Therefore, even when these interventions produce similar positive psychological effects, the effects may be very different on physiological stress processes. One factor that may have particular salience is that amount of physiological arousal produced by the intervention.

Adolescent↗

A test to estimate VO2max in females using aerobic dance, heart rate, BMI, and age.

OBJECTIVE: The purpose of this investigation was to develop a sub-maximal exercise test for estimating VO2max utilizing aerobic dance. EXPERIMENTAL DESIGN: One hundred females between the ages 18 to 40 yr served as the subjects for test validation. The subjects completed a treadmill test to determine VO2max and were assessed for heart rate (HR) response to a bout of aerobic dance. The data associated with responses to treadmill exercise and the aerobic dance test, in conjunction with descriptive variables (e.g., age, BMI) were utilized in the validation of the multiple regression model. MEASURES: Reliability was determined by correlation and paired "t"-tests of the aerobic dance routine test and retest trials. The construction of the multiple regression equation, via forward entry analysis, and the cross-validation of the regression equation were completed to ensure the validity and reliability of the protocol in accurately estimating VO2max. RESULTS: Test, retest reliability for the dance-exercise routine was demonstrated (r = 0.98). Moreover, no significant differences were shown between the HR responses for the test and retest trials. The multiple regression analysis yielded a three variable multiple prediction equation for estimating VO2max (R = 0.84; SEE, 5.5 ml.kg-1.min-1). The three variables were the HR response to four min of aerobic dance (HR4), body mass index (BMI), and age (years). Cross-validation of the aerobic dance test was determined with a second group of 50 female subjects (R = 0.83; SEE, 5.5 ml.kg-1.min-1). Additionally, the application of the validation group regression equation to the cross-validation group yielded a comparable R of 0.82. Comparison of the predicted values for VO2max from both equations also yielded a highly significant invariance coefficient of 0.96. Finally, the results of "t"-tests between the observed and predicted mean values for VO2max revealed no significant difference (p > 0.05). Therefore, the final prediction equation, based on collapsing the means associated with the validation and cross validation samples (n = 150), was: VO2max (ml.kg-1.min-1) = 130.18-(0.38 * HR4)-(0.81 * BMI)-(0.27 * Age); R = 0.84, SEE = 5.5 ml.kg-1.min-1. CONCLUSIONS: These results indicate that a four minute aerobic dance test provides a valid and reliable sub-maximal protocol for estimating VO2max and providing an index of aerobic fitness in apparently healthy 18 to 40 yr old females.

Adolescent↗

Effects of pollen quality and genotype on the dance of foraging honey bees.

Animals assess the quality and quantity of food and choose among different foods based on these assessments. We explored whether there was genetic variation for assessment of pollen quality by foraging honey bees, Apis mellifera. Honey bees derived from two genotypic strains foraged for pollen of varying quality from a petri dish placed inside an outdoor flight cage. The strains were the result of a colony-level, two-way selection on amount of stored pollen. We used the forager's round dance to quantify the assessments of pollen quality by individually marked worker bees. The dance rate (number of 180 degrees turns per minute) and the probability of dancing were each greater when bees foraged for pure pollen compared with a lower-quality mixture of pollen and alpha-cellulose (1:1 by volume). Bees from the high-pollen genotypic strain had a higher dance rate than those from the low-pollen strain, suggesting different assessments. Bees from the low-pollen strain, however, had a higher probability of dancing than did bees from the high-pollen strain. Dance duration was not affected by a bee's strain or by the quality of pollen. We conclude that the dance rate may be used to quantify a forager's subjective evaluation of pollen quality and that this evaluation has a genetic component. Our results also suggest that the dance may function at the colony level to recruit bees to more profitable pollen sources. Copyright 1998 The Association for the Study of Animal Behaviour.

Journal Article↗