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At least 37 records · Page 2Linked to original sources
PubMed1979

ELISA for the serology of FIP virus.

An enzyme linked immunosorbent assay (ELISA) for feline infectious peritonitis (FIP) virus serology is described. The assay is analogous to a previously developed indirect heterologous immunofluorescence test (IFT) in which transmissible gastroenteritis (TGE) viral antigen was used. Comparative testing of selected feline sera in both assays resulted in corresponding titers, which justifies the conclusion that the ELISA is a reliable test for the serology of FIP virus.

Animals↗
PubMed1979

A new principle for the detection of specific IgM antibodies applied in an ELISA for hepatitis A.

A new test principle for the detection of specific IgM-class antibodies was developed and applied in an Enzyme-Linked Immuno Sorbent Assay (ELISA) for the detection of hepatitis A IgM antibodies. A solid phase coated with anti-IgM was incubated successively with serum sample, specific antigen, and enzyme-labeled F (ab')2 fragments from IgG antibodies against the antigen and enzyme substrate. F(ab')2 fragments were used to avoid interference with rheumatoid factor. Specificity and sensitivity are very high. This test principle appears generally applicable in the diagnosis of infectious and parasitic diseases by testing only one serum sample.

Antibodies, Viral↗
PubMed1978

Rapid detection of IgG and IgM antibodies for cytomegalovirus by the enzyme linked immunosorbent assay (ELISA).

A simple solid phase enzyme immunoassay for the detection of immunoglobulin G and M to cytomegalovirus (CMV) is described. Using this test IgM antibodies to CMV were detected in 0.7 per cent of newborns and regularly after CMV infection in transplant patients, furthermore in these latter patients IgM production was prolonged for several months. For the determination of IgG the enzyme immunoassay was more sensitive than the complement fixation test (CF) and the antibody titres were 4 to 8 fold higher. Since the ELISA test is rapid, specific and unexpensive it can become an acceptable routine diagnostic procedure.

Adult↗
PubMed1979

Diffusion in gel-enzyme linked immunosorbent assay (DIG-ELISA): a simple method for quantitation of class-specific antibodies.

A new method for quantifying class-specific antibodies is presented. The method has been named Diffusion-In-Gel-Enzyme-Linked-ImmunoSorbentAssay (DIG-ELISA), and is briefly as follows. Antiserum ia allowed to diffuse from wells in a gel layered over an antigen-coated plastic surface. The gel is then removed and the preparation is incubated with enzyme-conjugated anti-immunoglobulin. The enzyme is then visualised in situ by a colour reaction produced by pouring a substrate-containing gel over the plastic surface. Bovine serum albumin and rabbit-anti-BSA were used as a model system, and horseradish peroxidase or alkaline phosphatase as enzymes for visualization.

Alkaline Phosphatase↗
PubMed1979

Detection of antibodies to Sendai virus by enzyme-linked immunosorbent assay (ELISA).

An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Sendai virus, a paramyxovirus, is described. The assay was found to be about 20-fold more sensitive than the hemagglutination inhibition assay. Differentiation between virus specific IgG and IgM is possible. The test appears to be especially useful in the study of early events in antibody formation in vivo as well as in vitro.

Animals↗
PubMed1979

ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes.

A method is described which permits the adaption of ELISA techniques for measurement of antibody against bacterial polysaccharides. First, the polysaccharides antigen is covalently bound to poly-L-lysine, using cyanuric chloride as the coupling agent. The poly-L-lysine then adsorbs to the walls of plastic tubes, thus immobilizing the polysaccharide coupled to the poly-L-lysine. The method is simple, rapid, and utilizes small amounts of polysaccharide antigen.

Adsorption↗
PubMed1978

ELISA: enzyme-linked immunosorbent assay.

Similar in design to radioimmunoassay, comparable in sensitivity and specificity but easier, safer, and less expensive, this new diagnostic technique uses enzyme-labeled rather than isotope-labeled reagents. The end point is a color change that can be assessed by colorimetry or with the naked eye. Various techniques of ELISA are described, along with examples of current and potential clinical applications.

Animals↗
PubMed1978

Enzyme immunoassays with special reference to ELISA techniques.

In this review outlines are given on various types of enzyme immunoassay. The applications to such enzyme immunoassays, especially ELISA, are dealth with in detail. It is concluded that these techniques have high sensitivity and will be suitable in due course as routine laboratory tests.

Antibodies↗
PubMed1978

The detection of rotavirus specific antibody in colostrum and milk by ELISA.

The blocking method of ELISA for the detection and titration of rotavirus-specific antibody in colostrum is described. The results obtained were positively correlated with those of a neutralizing antibody test. On one farm colostrum samples were obtained over a period of 18 months. No relationship was found between the titer of colostrum obtained shortly after calving, and the development of rotavirus-associated diarrhoea in calves. On a second farm only samples obtained during the calving season were tested. Within this restricted period high colostral antibody titers appeared to reduce the incidence of diarrhoea among calves and to delay the onset of rotavirus excretion in the faeces. These results are discussed in relation to the rapid decline in antibody content of colostrum after calving.

Animals↗
PubMedDate not supplied

[Comparative value of ELISA technique and immunofluorescence for the detection in the foci of human African trypanosomiasis].

As the parasitological diagnosis method consisting in the detection of the parasite in people suffering from sleeping sickness did not prove entirely reliable, the authors have tested a new immunoenzymatic diagnosis method called ELISA. 41 sera from clinical suspects have been tested (parasite having been found in 16 of them). Positive and negative levels have been tested by comparison with indirect immunofluorescence. The following results, given in optical density, have been obtained: negative cases less than 36 less than or equal to uncertain cases less than or equal to 40 less than positive cases. When using these date, correlation with immunofluorescence proves to be excellent with a sensibility between 95 and 97,5 p. 100 and a specificity between 97,5 and 99 p. 100.

Cote d'Ivoire↗
PubMed1978

Class-specific antibody titres (ELISA) against the primary immunogen Helix pomatia haemocyanin (HPH) in man.

Using the indirect ELISA technique, IgM, IgG and IgA antibody titres against the primary test immunogen Helix pomatia haemocyanin (HPH) could be measured. All twenty-seven normal volunteers (age range 26-74 years) developed maximal or high IgM, IgG and IgA antibody titres 2 weeks after primary immunization with 1.0 mg HPH subcutaneously. Lower dose immunization resulted in submaximal responses. Titre kinetics in the three Ig classes correlated in height and time per person. There was no significant difference in antibody response between two adult age groups. Secondary immunization after 6 weeks, again with 1.0 mg HPH, when high titres were still present, resulted in a small titre increase in all three Ig classes. Comparison with the tanned red cell agglutination technique used previously, in combination with the 2-mercaptoethanol treatment of sera, showed the unreliability of 7S titres measured thus early in the primary immune response. Investigation of four patients with humoral immunodeficiency confirmed the class specificity of the test.

Adult↗