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PubMed1976

Activities of oxidative enzymes in thyroid follicles of Xiphophorin fishes.

The activity of some intracellular oxidative enzymes was studied histochemically in the cells of the thyroid follicles of teleost fishes of the genus Xiphophorus. The experimental material consisted of animals of the red swordtail and Mexican swordtail breeds of Xiphophorus helleri and of melanotic Xiphophorus maculatus fishes. Observations were carried out on adult specimens of both sexes, including pregnant femals of Mexican swordtail. Moreover, immature Mexican swordtails of both sexes were examined. Thyroid follicles were found to be present in the subpharyngeal region of all fishes studied. The distribution of these follicles as well as their number and form depended on sex, age and on the analysed stage of prenancy. A smaller number and size of thyroid follicles were characteristic of immature specimens, whereas they were most numerous in the thyroids of pregnant fishes. The follicles were arranged in characteristic dense aggregations, especially in the melanotic platyfish. The follicular eipthelium in the fishes under study was usually cubical, but pregnant and non-pregnant adult females also contained a considerable number of larger follicles with flattened epithelium. Besides, thyroid follicles of multilayer epithelium were rather frequently encountered, especially in male fishes, irrespective of their age. The thyroid follicle cells of these fishes demonstrated invariably high activities of reduced NAD and NADP dehydrogenases and of beta-hydroxybutyrate dehydrogenase, and a low activity of succinat dehydrogenase. The intensities of alpha-glycerophosphate and lactate dehydrogenases and of cytochrome oxidase varied with sex, age and breed of the studied fishes. The immature and pregnant fishes showed the most clearly pronounced differences in the intensity of enzymic activity, the thyroid follicles of immature specimens revealing a high activity of lactate dehydrogenase and low activity of cytochrome oxidase, an inverse picture being seen in pregnant fishes. The adult forms of both sexes exhibited an enhanced activity of cytochrome oxidase and a decline in that of lactate dehydrogenase. The observed differences in the intensities of enzymic acitivities in the thyroids of the studied fishes are related with functions of this gland which in the period of growth are different from those in the period of sexual maturity, and certainly also with individual metabolic characteristics of the studied fishes.

Age Factors↗
PubMed1979

Clostridium botulinum in fish.

1407 fish caught in Scandinavian waters, the North Sea and the North Atlantic have been examined for the presence of Cl. botulinum. The incidence in gut samples expressed as percentage of fish tested was generally highest in fish from Scandinavian coastal waters and the Baltic Sea (4--43%), decreasing in fish from the North Sea (0--8%), and the organism was practically absent in fish from the North Atlantic. When gut samples were examined, the incidence was highest in demersal fish (cod and flatfish) as compared with pelagic fish (herring). The latter fish species were mainly contaminated on outer surfaces and gills. Only type E was detected in this survey, and Cl. botulinum was not detected in any wild fresh water fish. It is suggested that type E spores may originate in the sea bed and that they be spread by fish and water currents.

Animals↗
PubMed2026

Proteobacteria with chemosynthetic potential are highly prevalent in the gills of Hypoplectrus reef fishes.

Fishes host a diverse microbiome in their gills, but a broad characterization of this microbiome at the metagenomic level is lacking. Here, we apply genome-resolved metagenomics to the gills of the hamlets (Hypoplectrus spp), a group of reef fishes from the Greater Caribbean. The analysis of 353 gill samples from 15 hamlet species collected at eight locations over 13 years revealed a stark contrast between the gill microbiota and reef water microbial communities, indicating a distinct and specific gill microbiome. A total of 70 gill-associated metagenome-assembled genomes (MAGs) were recovered. These MAGs belong to 17 lineages, most of which are novel. They relate to known fish gill pathogens, fish gut microbes, free-living and biofilm-associated taxa, indicating that the gill microbiome was assembled from a collection of distinct eco-evolutionary trajectories. The MAGs harbor diverse metabolic modules, involved notably in nitrogen cycling, antibiotic production and biofilm formation, revealing a highly dynamic microbial ecosystem. One lineage in the Burkholderiaceae family was outstandingly prevalent across fish host species, sampling locations and years. Its genome encoded complete metabolic modules for carbon fixation and sulfur oxidation, indicating chemosynthetic potential. To the best of our knowledge, this is the first line of evidence that fishes may host sulfur-oxidizing chemosynthetic bacteria in their gills. The functional significance of this chemosynthetic potential for the fish host or other members of the gill microbiome remains to be established. The high prevalence of this lineage allowed to build a pangenome. It revealed large-scale geographic structure (western Caribbean, eastern Caribbean and Gulf of Mexico), which parallels the phylogenomic pattern observed in the hamlets. Overall, our findings point to complex fish host-microbe and microbe-microbe eco-evolutionary interactions in the gills that may influence fish physiology, homeostasis and immune response.

Animals↗
PubMed1979

Response from menhaden fish meal in diets of young turkeys.

Diets involving three or five fish meal treatments and two zinc bacitracin treatments were fed to 5410 poults. The poults were divided into 552 pens and raised to two, three or four weeks of age in five series of 3 X 2 or 5 X 2 factorial experiments. Body weights increased 9.0 to 31.3% from the addition of 5% menhaden fish meal and 5.4 to 11.9% from the addition of 44 ppm bacitracin. No interaction existed between fish meal and bacitracin. Residues derived from either water or petroleum ether extraction of fish meal, when added to diets at levels equivalent to those in 5% fish meal, produced body weight gains nearly equal to those obtained from 5% fish meal. Body weights were not increased by the addition of either water or petroleum soluble extracts or by charred or ashed fish meal. The addition of a mixture of crystalline amino acids, defluorinated phosphate, and fat equivalent to 5% fish meal produced only a 4.4% increase in body weight. An unidentified growth factor which is non-lipid, non-mineral, and non-water soluble appears to be present in menhaden fish meal when added to well foritified po

Amino Acids↗
PubMedDate not supplied

[Amino acid composition of protein products from fish of low market value].

The amino acids composition of proteins from the whole fry fish, proteinic mass and the fish protein concentrate was investigated by using ion-exchange chromatography with an automatic analyzer (AAA-881, ChSSR). A balance of amino acids recorded during preparation of the protein mass obtained in fish with inferior market value (fry herring and sand fish) is cited. It was found that the protein mass of fry contains 2.3 and that of sand-fish, 1.3 as much of the amino acids as does the whole fish. It is methionine that has been ascertained to be a limiting amino acid for fry and the products of its processing. In the protein of this fish essential amino acids comprise 43.5% of the sum-total of all the amino acids, the corresponding percentages in the protein mass and the fish protein concentrate being 42.1 and 41.7, respectively. A comparison of the amino acids composition of the study proteins as against the amino acid scale of the ideal FAO protein shows that the protein mass and the fish protein concentrate represent products of high biological value.

Amino Acids↗
PubMed2025

TRIM63 Overexpression in FISH-Negative MiTF Family Altered Renal Cell Carcinoma (MiTF RCC).

TFE3 and TFEB break-apart fluorescent in situ hybridization (FISH) assays are the "gold standard" for diagnostic confirmation of microphthalmia-associated transcription factor (MiTF) family-altered renal cell carcinoma (MiTF RCC), which includes TFE3-rearranged RCC and TFEB-altered RCC. However, FISH assays, for multiple reasons, may lead to equivocal or false-negative results, especially in cryptic fusions resulting from intrachromosomal inversions involving 5' partner genes, such as non-POU domain-containing octamer-binding protein (NONO); GRIPI-associated protein 1 (GRIPAP1); RNA-binding motif protein, X chromosome (RBMX); and RNA-binding motif protein 10 (RBM10). When FISH results are negative in cases with strong morphological suspicion of the listed tumor entities, pathologists may recommend targeted RT-PCR or panel-based RNA fusion sequencing for diagnostic confirmation. Our recent RNA in situ hybridization (RNA ISH)-based study demonstrated RNA expression of the tripartite motif containing 63 (TRIM63) to be highly enriched in TFE3-rearranged RCC and TFEB-altered RCC, including 2 FISH false-negative RCC cases harboring RBM10::TFE3 fusion. Based on these observations, we hypothesized that TRIM63 positivity could aid in diagnosing cases that are negative by conventional FISH assay but remain morphologically suspicious, representing an unmet clinical need in this area. We collected 20 RCC cases with morphological suspicion (with equivocal/indeterminate immunohistochemistry panel) of MiTF RCC, which were TRIM63 positive, negative/equivocal for TFE3/TFEB gene rearrangement by FISH, and underwent next-generation sequencing (NGS). On NGS correlation, 14 of 20 (70%) FISH-negative TRIM63-positive tumors harbored an MiTF gene rearrangement. In the remaining 6 cases, we were unable to fully ascertain the MiTF rearrangement status due to the inherent limitation of the NGS panel utilized. The cases with MiTF gene rearrangement include TFE3 rearrangement in 60% (12/20) and TFEB low-level copy gains (with an additional missense mutation in 1 case) in 10% (2/20) of samples. RBM10:TFE3 fusion was seen in 67% (8/12) of TFE3-rearranged RCC in this cohort. TRIM63 RNA ISH assay could aid in identifying cases that harbor TFE3 or TFEB rearrangement associated with false-negative or equivocal TFE3/TFEB FISH results, especially those involving gene fusions with a paracentric Xp11 inversion. Overall, employment of TRIM63 RNA ISH coupled with TFE3/TFEB FISH assays and follow-up genomic interrogation enhanced diagnostic accuracy for patients with MiTF RCC.

Carcinoma, Renal Cell↗
PubMed1975

Fish health--some concepts, constraints, and comparisons.

This has been an overview of fish health, a comparison of some aspects of fish husbandry with other animal husbandry, and comments concerning fish health research--especially where it is going. The 1972 disease survey identified current problems; it ranked the problems according to priority. More important, research effort has been redirected. Problems of highest priority are receiving the greatest attention; those of lesser priority are receiving the appropriately lesser attention. Just as veterinary and human medical research have become more mission-oriented, fish health research has also become much more of a problem-solving activity. There are fewer dollars; they must be used wisely and they must produce. Research in fish health is expected to yield usable information that will be of significant help in producing better fishes. If destined for the table, such fishes should convert feeds rapidly and efficiently, and they should not suffer losses from diseases. If intended for stocking, such fishes should be capable of thriving in the environment and of surviving to fulfill their destiny.

Animal Husbandry↗
PubMed1977

Poecilancistrium caryophyllum and other trypanophynch cestode plerocercoids from the musculature of Cynoscion nebulosus and other sciaenid fishes in the Gulf of Mexico.

Examination of over 3,000 fish, all sciaenids, has shown that plerocercoids of Poecilancistrium caryophyllum infect Cynoscion nebulosus, Bairdiella chrysura, Sciaenops ocellata, C. arenarius, Micropogonias undulatus, and Pogonias cromis in Mississippi Sound. In addition to P. caryophyllum, a pseudophyllidean-like trypanorhynch also infected C. nebulosus and M. undulatus. Pseudogrillotia pleistacantha selectively infected large P. cromis, Pterobothrium heteracanthum infected M. undulatus, and Pterobothrium lintoni infected Menticirrhus americanus. Prevalence and intensity of infections of P. caryophyllum in C. nebulosus from Mississippi, roughly 40% of the fish each possessing an average of about two worms, compare with infections observed in fish from Texas and Louisiana. However, values for fish from Apalachee Bay, but not Tampa Bay, Florida, are about twice as high. Extensive seasonal sampling in Mississippi Sound indicates fluctuations but no clear-cut seasonal trends other than a possible relationship between infections and salinity. As C. nebulosus increases in length, the prevalence of infections, but not the intensity, increases. This finding suggests an immune response to challenge infections. No fish less than 140 mm SL and relatively few less than 250 mm SL revealed infections, suggesting either the unavailability of the intermediate host to young fish or the ultimate death of most young infected individuals. Based on condition coefficients and liver-weight analysis, no apparent detrimental effect on infected, moderately-heavy adult fish occurred. Infections primarily involve the middle of a fillet or the region adjacent to the vertebral column below the dorsal fins and cause an esthetically displeasing product and consequently depress economically the trout fishery. Relatively more male than female fish harbored worms, but the significance of that finding is questioned.

Animals↗
PubMed1977

Mercury levels in freshwater fish of the state of South Carolina.

Samples of fish from freshwater sources of rivers, lakes and ponds all over the state of South Carolina were collected during the Summer of 1974 and 1975. The fish collected were Bass, Bluegill, Redbreast, Catfish, Shad, Carp, Crappie, Mudfish and Pike. Samples were analyzed using the flameless atomic absorption procedure outlined by Hatch and Ott, and Uthe et al as modified for use with Perkin-Elmer, Coleman MAS-50 mercury analyzer. Triplicate samples of fish tissue were analyzed by wet digestion method. The mean mercury levels in ppb were determined for baseline mercury levels. A significant finding of this report is that those species for which fish of widely differing weights were analyzed, larger fish had higher mercury levels. Mercury levels exceeding the U.S. Food and Drug Administration guideline of 500 ppb for fish tissues have been found in the Mudfish from Edisto River and Pike fish from Lake Murray. Higher levels of mercury occurred in the highly vascularized blood tissues of liver and kidney than in muscle. Carnivorous and bottom-feeding fishes are the most reliable indicators of mercury pollution.

Animals↗