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[DNA prints instead of plantar prints in neonatal identification].

BACKGROUND: To check the possible usefulness in studying DNA in dried blood spots taken on filter paper blotters for newborn identification. It set out to establish: 1. The validity of the method for analysis; 2. The validity of all stored samples (such as those kept in clinical records); 3. Guarantee of non-intrusion in the genetic code; 4. Acceptable price and execution time. MATERIAL AND METHODS: Forty (40) anonymous 13-year-old samples of 20 subjects (2 per subject) were studied. DNA was extracted using Chelex resin and the STR ("small tandem repeat") of microsatellite DNA was studies using the "polimerase chain reaction method" (PCR). Three non coding DNA loci (CSF1PO, TPOX and THO1) were analyzed by Multiplex amplification. RESULTS: It was possible to type 39 samples, making it possible to match the 20 cases (one by exclusion). The complete procedure yielded the results within 24 hours in all cases. The estimated final cost was found to be a fifth of that conventional maternity/paternity tests. CONCLUSIONS: The study carried out made matching possible in all 20 cases (directly in 19 cases). It was not necessary to study DNA coding areas. The validity of the method for analyzing samples stored for 13 years without any special care was also demonstrated. The technic was fast, producing the results within 24 hours, and at reasonable cost.

DNA Fingerprinting↗

Patterns and costs of printed and online journal usage.

This study in an academic medical sciences library setting examines the correlation of usage of a matched set of print and online titles, the validity of e-journals usage statistics and the impact of online journals on print journal usage. The print and online usage was determined for 270 journals, both versions of which were available. Print usage was determined annually since 1997 using the reshelving and the error-copies method. Online usage statistics were delivered by five publishers and corrected for redundant multiple accesses. Print journal usage decreased by 22.3 and 30.2% respectively over each of 2 years after the introduction of online journals. Journals published both in print and online lost 30.4% of their print usage within 2 years. The total loss of usage of print-only titles in the same period was somewhat higher, at 45.8%. The average correlation between online and print usage is 0.60 and 0.67 respectively. For the examined titles, users accessed the online versions ten times as often as the print version. Two clearly distinguishable groupings emerged: while with Academic Press and Elsevier, e-journal usage exceeded print usage by a factor of 3 or 4, the e-journals of Blackwell, HighWire and Springer were used on average 14.6 times as frequently as the corresponding print journals. Each usage of a print article cost 2.79-50.82 Euro, each usage of an online article 0.31-15.10 Euro, depending on the publisher. On average, the usage of an online article was 5.4 times cheaper. Within 2-3 years the usage of online journals has outstripped that of print titles by a factor of ten, but the specific spectrum of usage remains much the same as when only print journals alone existed. Print titles not available online suffer a greater decline in usage compared with print/online journals. This confirms that what is read or purchased is determined primarily by ease of access and that there is a steady tendency to reduce the multiplicity of access modes to a manageable few. The availability of journals online seems to have created a new clientele, at least in the case of the German-language Springer journals. The connection between supplier and supply is much less clear with e-journals than it is with print titles. Therefore it is very important to stress and encourage the role of the library as the supplier of this sort of information in the university environment. Collection building issues are discussed in the light of the results.

Costs and Cost Analysis↗

Electronic journal access: how does it affect the print subscription price?

OBJECTIVE: This study examined the rates of print journal subscription price increases according to the type of available electronic access. The types of access included: electronic priced separately from the print, combination print with "free online" access, and aggregated, defined here as electronic access purchased as part of a collection. The percentages of print price increases were compared to each other and to that for titles available only in print. The authors were not aware of prior objective research in this area. METHODS: The authors analyzed the percentage print price increases of 300 journals over a five-year time period. The titles were grouped according to type of available electronic access. The median and mean percentage print price increases were calculated and plotted for all titles within each group. RESULTS: Using both the median and the mean to look at the percentage print price increases over five years, it was obvious that print prices for journals with electronic access exceeded journals that did not offer an electronic option. Electronic priced separately averaged 3% to 5% higher than print only titles using both measures. Combination print with "free online" access had higher increases from 1996 to 1999, but, in 2000, their percentage increases were about the same as print only titles. The rate of price increases for aggregated titles consistently went down over the past five years. Journals with no electronic option showed the lowest percentage rates of print price increase. CONCLUSIONS: The authors' findings reveal that the increases of print prices for their sample of titles were higher if a type of electronic access was offered. According to the results of this study, aggregated collections currently represent the electronic option whose percentage price increase for print prices was lowest. However, the uneven fluctuations in rates of subscription prices revealed that the pricing of journals with electronic access is still evolving. More study is recommended to see if the trends observed in this study are sustained over a longer time period.

Copyright↗

Psychophysics of reading. XVIII. The effect of print size on reading speed in normal peripheral vision.

Reading in peripheral vision is slow and requires large print, posing substantial difficulty for patients with central scotomata. The purpose of this study was to evaluate the effect of print size on reading speed at different eccentricities in normal peripheral vision. We hypothesized that reading speeds should remain invariant with eccentricity, as long as the print is appropriately scaled in size--the scaling hypothesis. The scaling hypothesis predicts that log-log plots of reading speed versus print size exhibit the same shape at all eccentricities, but shift along the print-size axis. Six normal observers read aloud single sentences (approximately 11 words in length) presented on a computer monitor, one word at a time, using rapid serial visual presentation (RSVP). We measured reading speeds (based on RSVP exposure durations yielding 80% correct) for eight print sizes at each of six retinal eccentricities, from 0 (foveal) to 20 deg in the inferior visual field. Consistent with the scaling hypothesis, plots of reading speed versus print size had the same shape at different eccentricities: reading speed increased with print size, up to a critical print size and was then constant at a maximum reading speed for larger print sizes. Also consistent with the scaling hypothesis, the plots shifted horizontally such that average values of the critical print size increased from 0.16 deg (fovea) to 2.22 deg (20 deg peripheral). Inconsistent with the scaling hypothesis, the plots also exhibited vertical shifts so that average values of the maximum reading speed decreased from 807 w.p.m. (fovea) to 135 w.p.m. (20 deg peripheral). Because the maximum reading speed is not invariant with eccentricity even when the print size was scaled, we reject the scaling hypothesis and conclude that print size is not the only factor limiting maximum reading speed in normal peripheral vision.

Adolescent↗

The influence of polymer type, print donor and age on the quality of fingerprints developed on plastic substrates using vacuum metal deposition.

This study investigated fingerprint development on five different polymer substrates using vacuum metal deposition (VMD). The conditions required for optimum development are shown to depend on a number of factors. No one set of conditions will result in good development in all situations. Polymer type has been confirmed as a major factor in determining the types of development that will occur and the optimum VMD conditions required. For more consistently successful VMD development, polymer type should be determined before selecting conditions. While polymer type is a key factor in determining optimum development conditions, there may be variation of the optimum conditions within a polymer type, most likely due to the presence of additives in the plastic. The heaviness of a latent print, i.e. amount of residue that constitutes the print, also affects the VMD conditions required. The donor, manner of deposition, and age of a print affect the heaviness of the deposit. The heavier the print, the higher the gold count necessary for successful VMD development. The occurrence of 'empty prints' (i.e. zinc deposition on the general background but not on or between the print ridges) was found to be related to polymer type and print heaviness. Heavy prints on PVC and PET are the most likely to be 'empty' after VMD treatment. The development of empty prints may be due to the diffusion of print residue into the print valleys. Pre-treatment with cyanoacrylate fuming was also found to affect VMD development. In particular, it was shown that cyanoacrylate pre-treatment was beneficial for print development on PET and PVC. The results of this study were used to formulate guidelines for use as an aid by laboratories using VMD in casework.

Dermatoglyphics↗

Development of print awareness in language-disordered preschoolers.

This study examined print awareness and related oral language abilities in language-disordered and normally developing preschoolers. Twenty subjects, ages 3:1 to 6:5 (years:months), were shown high frequency environmental print in four conditions varying in the amount of non-print information present in the print setting. They were asked to match the print to the object that it signified and to provide verbal labels for the same objects. Results indicated that normal-language children were responding meaningfully to print settings that contained reduced non-print cues while the language-disordered subjects were not. General language ability was correlated with print awareness, but knowledge of specific oral lexemes was not necessary for accurate print responses. Parent questionnaire data suggested that group differences did not result from differential prior experience with the print items. Results are discussed with reference to hypothesized relationships between oral and written language.

Awareness↗

Developing of EEG print and its preliminary technical application.

In this report, we discuss a method called "EEG print" to represent EEG contrast mapping in time and frequency domains simultaneously. A bank of bandpass FIR (Finite Impulse Response) digital filters is used to obtain EEG prints. EEG prints were taken from four areas (F3, F4, O1 and O2) of EEG when healthy subjects were at rest with their eyes closed. The pattern of the prints was classified into four types: alpha type, beta type, alpha + beta type and complex type. It was found that EEG prints may vary from person-to-person but they usually do not vary much between the four areas for a given person. The method is further modified to obtain "differential EEG prints" to investigate whether meaningful higher frequency EEG components exist. Differentiation of EEG resulted in marked intensification of the fast waves, using 0.14 Hz as the critical point. In differential EEG print with higher order differentiation, amplification in the high frequency components increase their frequency. As a result, it is possible to observe variations in the high frequency components, which are otherwise not detectable in the usual EEG print. EEG print can be used for representing the function of the brain. Using the method for classification of EEG print patterns, described in this paper, we can clarify not only the characteristics of the normal brain but also the pathophysiology of mentally-ill patients.

Alpha Rhythm↗

Cell and organ printing 2: fusion of cell aggregates in three-dimensional gels.

We recently developed a cell printer (Wilson and Boland, 2003) that enables us to place cells in positions that mimic their respective positions in organs. However, this technology was limited to the printing of two-dimensional (2D) tissue constructs. Here we describe the use of thermosensitive gels to generate sequential layers for cell printing. The ability to drop cells on previously printed successive layers provides a real opportunity for the realization of three-dimensional (3D) organ printing. Organ printing will allow us to print complex 3D organs with computer-controlled, exact placing of different cell types, by a process that can be completed in several minutes. To demonstrate the feasibility of this novel technology, we showed that cell aggregates can be placed in the sequential layers of 3D gels close enough for fusion to occur. We estimated the optimum minimal thickness of the gel that can be reproducibly generated by dropping the liquid at room temperature onto a heated substrate. Then we generated cell aggregates with the corresponding (to the minimal thickness of the gel) size to ensure a direct contact between printed cell aggregates during sequential printing cycles. Finally, we demonstrated that these closely-placed cell aggregates could fuse in two types of thermosensitive 3D gels. Taken together, these data strongly support the feasibility of the proposed novel organ-printing technology.

Animals↗

Evaluation of the image quality of ink-jet printed paper copies of digital chest radiographs as compared with film: a receiver operating characteristic study.

Paper copies of digital radiographs printed with the continuous ink-jet technique have proved to be of a high enough quality for demonstration purposes. We present a study on the image quality of ink-jet printed paper copies of digital chest radiographs, based on receiver operating characteristic (ROC) analysis. Eighty-three digital radiographs of a chest phantom with simulated tumors in the mediastinum and right lung, derived from a computed radiography (CR) system were presented in two series of hard copies as ink-jet printed paper copies and as laser recorded film. The images, with a matrix of 1,760 x 2,140 pixels, were printed with a spatial resolution of 10 pixels/mm in the CR film recorder as well as in the ink-jet printer. On film, every image was recorded in two versions, one optimized for the mediastinum and one for the lungs. On paper, only one image was printed; this constituted an effort to optimize both the mediastinum and the lungs. The ink-jet printed images, printed on a matt coated paper, were viewed as on-sight images with reflected light. The examinations were reviewed by six radiologists, and ROC curves were constructed. No significant difference was found between the performance of film and that of ink-jet paper prints. Because the cost for a paper copy is only a tenth of that of film, remarkable cost reductions can be achieved by using the ink jet technique instead. Our results show that further quality studies of ink-jet printed images are worthwhile.

Copying Processes↗

Effects of relative humidity and buffer additives on the contact printing of microarrays by quill pins.

DNA microarrays printed with quill pins exhibit significant variation in probe DNA spots. Interspot variations and nonuniform distribution of probe within spots are major sources of experimental uncertainty in microarray analysis. To gain better insight into the sources of variation, we analyzed 450 consecutive depositions printed at relative humidities between 40 and 80% using three print buffers. Increasing relative humidity improved printing performance by delaying pin failure but did not reduce the variability in spot characteristics. Adding either betaine or dimethyl sulfoxide (DMSO) to the print buffer also improved quill pin performance. Least interspot variation was observed with the DMSO additive printed at 80% relative humidity, but this additive also resulted in the greatest intraspot variation. Least intraspot variation was observed with 1.5M betaine printed at 60% relative humidity, but these conditions produced microarrays with high interspot variability. Evaporation of printing solution from the quill reservoir appeared to be the primary cause of interspot and intraspot variations. Our studies indicate that relative humidity and printing solution additives reduce evaporation. Based on the spot variability requirements for a particular application, humidity and additives may be chosen to optimize either inter- or intraspot variability.

Buffers↗

Effectiveness of a printed leaflet for enabling patients to use digoxin side-effect information.

The effectiveness of a printed leaflet designed to inform patients about digoxin was evaluated. The printed leaflet alone was compared to the verbal consultation alone and the verbal consultation in addition to the printed leaflet. The study assessed patients' decisions about the appropriate course of action to take if side-effect symptoms occurred. Patients receiving the printed leaflet alone scored higher than patients receiving the verbal consultation; however, they scored lower than patients receiving both the printed leaflet and verbal consultation together. Patients receiving both verbal and printed information scored significantly higher than those receiving verbal consultation only. The findings suggest that printed materials together with verbal consultation are essential for enabling patients to make appropriate decisions with respect to side effects. There is not enough evidence to support the use of printed materials in place of verbal consultation. Health practitioners should use printed materials as an adjunct to verbal information.

Digoxin↗

Application of Three-Dimensionally Printed Surgical Guides in Precise Sacral Tumor Excision and Defect Reconstruction.

OBJECTIVE: Precise resection of sacral tumors remains technically demanding due to their deep anatomical location and close proximity to critical neurovascular structures. Conventional freehand techniques often result in suboptimal resection margins, excessive blood loss, and compromised lumbopelvic stability. This study evaluated whether patient-specific three-dimensional (3D)-printed guiding templates improve surgical accuracy and perioperative outcomes in sacral tumor resection and reconstruction. METHODS: Nineteen patients undergoing en bloc sacral tumor resection (S1-S3 involvement) with spinopelvic reconstruction (2006-2020) were retrospectively analyzed. Patients were divided into a 3D-printing group (n&#x2009;=&#x2009;10) and a conventional freehand group (n&#x2009;=&#x2009;9). In the 3D-printing group, computer-aided design and 3D-printed templates were used for osteotomy, screw placement, and defect reconstruction. Perioperative metrics, surgical accuracy, and complications were compared between groups using Welch's t-test and the Hodges-Lehmann method; oncologic events during follow-up were recorded descriptively. RESULTS: The 3D-printing group demonstrated significantly shorter operative time (456.5&#x2009;&#xb1;&#x2009;62.36 vs. 574.44&#x2009;&#xb1;&#x2009;114.58&#x2009;min, p&#x2009;=&#x2009;0.012), reduced blood loss (4081.40&#x2009;&#xb1;&#x2009;838.99 vs. 5090.0&#x2009;&#xb1;&#x2009;1059.67&#x2009;mL, p&#x2009;=&#x2009;0.034), and fewer fluoroscopic exposures (4.2&#x2009;&#xb1;&#x2009;0.79 vs. 10.0&#x2009;&#xb1;&#x2009;1.58, p&#x2009;<&#x2009;0.001) compared with the conventional group. Osteotomy accuracy was also superior in the 3D-printing group, with significantly lower angular deviation (3.33&#xb0;&#x2009;&#xb1;&#x2009;0.45&#xb0; vs. 6.79&#xb0;&#x2009;&#xb1;&#x2009;2.16&#xb0;, p&#x2009;=&#x2009;0.0012). Postoperative complication rates were comparable (30% vs. 44.4%, p&#x2009;=&#x2009;0.649), but hospital stay was significantly shorter in the 3D-printing group (10.7&#x2009;&#xb1;&#x2009;2.71 vs. 18.11&#x2009;&#xb1;&#x2009;4.01&#x2009;days, p&#x2009;<&#x2009;0.001). CONCLUSION: Patient-specific 3D-printed guiding templates enhance precision in sacral tumor excision and reconstruction, improving surgical efficiency and perioperative safety. This computer-assisted, template-guided approach represents a valuable advancement for complex sacral oncologic surgery.

Humans↗

Subjective image quality of digital panoramic radiographs displayed on monitor and printed on various hardcopy media.

The subjective image quality of panoramic radiographs shown on a diagnostic computer monitor were compared with professional direct thermal prints and with common inkjet prints on different paper qualities. Indirect digital panoramic radiographs were obtained from 15 patients. The images were printed with a direct thermal printer in their original format. Afterwards, these were loaded in an imaging software programme (Microsoft Photo Editor) and assessed both on computer monitor and inkjet prints on transparency, glossy, satin and regular paper. Five observers assessed subjective image quality for different regions and anatomical landmarks on a 5-point rating scale. Data were statistically analysed and inter- and intra-observer performances were calculated. Best image quality was obtained with direct thermal prints, followed in descending order by panoramic images viewed on the monitor, inkjet prints on transparencies, glossy paper, satin paper and finally regular paper. The differences were significant except for monitor images versus direct thermal prints, inkjet-transparencies and inkjet-glossy images and inkjet-satin versus inkjet-glossy images. The subjective image quality of indirect digital panoramic images is different for images shown on the computer monitor and for printed images depending on both the printer and paper type used.

Adolescent↗

Fused Deposition Modeling (FDM) of polyether-ether-ketone (PEEK) dental implants: A systematic review of the effect of printing parameters on mechanical behaviour and surface quality.

PURPOSE: This systematic review evaluated how FDM printing parameters influence mechanical behaviour and surface characteristics of 3D-printed PEEK and identified parameter combinations linked to the most favourable mechanical performance and surface quality. MATERIALS AND METHODS: An electronic search was conducted in: MEDLINE (Ovid), PubMed, Embase, Web of Science, Scopus, and Compendex (last update: January 2025). Studies that evaluated the effect of FDM printing parameters on mechanical and surface properties of PEEK were included. Outcomes comprised compressive, tensile, and flexural strengths, elastic modulus, fracture toughness, surface hardness, roughness, and wettability. RESULTS: Of 4005 reports screened, 54 manuscripts were included. 92.6% (n&#x202f;=&#x202f;50) of articles showed low risk-of-bias, while 7.4% (n&#x202f;=&#x202f;4) showed medium risk-of-bias. Tensile strength was the most investigated mechanical parameter (78%), followed by elastic modulus (41%), flexural strength (30%), compressive strength (20%), and fracture toughness (6%). Surface roughness was the most evaluated surface property (30%), followed by hardness (17%) and wettability (6%). Across studies, higher printing temperatures, lower printing speed, thinner layer thickness, and maximum infill ratio in a horizontal printing orientation were associated with higher strengths, less warpage, increased accuracy, and improved surface quality. CONCLUSION: Specific combinations of FDM printing parameters can significantly improve the mechanical and surface properties of PEEK. However, it is difficult to meet all the optimal conditions simultaneously. Thus, balancing between different parameters must be considered in practical production.

Benzophenones↗

How effective is tailored print communication?

This article reviews the "first generation" of tailored print communications studies in the published literature, describing the purpose, theoretical framework, sample, research design, message type and source, outcomes measured, and findings of each. Eight studies compared tailored versus similar nontailored print; one compared tailored print versus an alternate intervention, and three included tailored print as one of several intervention components. Although studies varied by behavioral topic, type of tailoring, and measurement of behavioral outcomes, several themes persist. Compared to their nontailored counterparts, tailored print communications have been consistently better remembered, read, and perceived as relevant and/or credible. There is also evidence that tailored print communications are more effective for influencing health behaviors. Six of the eight tailored/nontailored comparisons found more behavior change among tailored than nontailored recipients. Tailored print communications have also demonstrated effectiveness as an adjunct to other intervention components such as self-help smoking cessation manuals. However, studies comparing tailored print communications with tailoring via other media such as telephone counseling have shown mixed results. Additional research is needed to assess whether the behavioral topic itself may make a difference in whether tailoring is appropriate and effective.

Health Behavior↗

Effects of bilingualism and biliteracy on children's emerging concepts of print.

Three groups of 4- and 5-year-old children were examined for their concepts of how print refers to language. All of the children could identify printed letters and their sounds but not read alone. The groups studied were monolingual speakers of English, bilingual speakers of French and English, and bilingual speakers of Chinese (Mandarin) and English. Bilingual children were equally proficient in both languages and were familiar with print and storybooks in both languages. The tasks assessed children's understanding of the general correspondence between print and language in which the printed form represents a word and the specific correspondence between a constituent of print and one of language that determines representation in a given writing system. The general correspondence relation applies to all writing systems, but the specific correspondence relation changes for different kinds of writing systems. Bilingual children understood better than monolingual children the general symbolic representation of print. The older Chinese-English bilingual children also showed advanced understanding of the specific correspondence relations in English print.

Attention↗

Mechanical properties of dense polylactic acid structures fabricated by three dimensional printing.

Polylactic acid (PLA) is a bioresorbable polymer that is used in a number of clinical situations. Complex shapes of PLA are commonly machined for bone fixation and reconstruction. Solid free from fabrication methods, such as 3D printing, can produce complex-shaped articles directly from a CAD model. This study reports on the mechanical properties of 3D-printed PLLA parts. 3D printing is a solid free-form fabrication process which produces components by ink-jet printing a binder into sequential powder layers. Test bars were fabricated from low and high molecular weight PLA powders with chloroform used as a binder. The binder printed per unit line length of the powder was varied to analyze the effects of printing conditions on mechanical and physical properties of the PLA bars. Furthermore, cold isostatic pressing was performed after printing to improve the mechanical properties of the printed bars. The maximum measured tensile strength for the low molecular weight PLLA (53 000) is 17.40 +/- 0.71 MPa and for high molecular weight PLLA (312 000) is 15.94 +/- 1.50 MPa.

Binding Sites↗

Online journals: impact on print journal usage.

PURPOSE: The research sought to determine the impact of online journals on the use of print journals and interlibrary loan (ILL). SETTING: The Library of the Health Sciences-Peoria is a regional site of the University of Illinois at Chicago (UIC) Library with a print journal collection of approximately 400 titles. Since 1999, UIC site licenses have given students and faculty affiliated with UIC-Peoria access to more than 4,000 online full-text journal titles through the Internet. METHODOLOGY: The Library of the Health Sciences-Peoria has conducted a journal-use study over an extended period of time. The information collected from this study was used to assess the impact of 104 online journals, added to the collection in January 1999, on the use of print journals. RESULTS: Results of the statistical analysis showed print journal usage decreased significantly since the introduction of online journals (F(1,147) = 12.10, P < 0.001). This decrease occurred regardless of whether a journal was available only in print or both online and in print. Interlibrary loan requests have also significantly decreased since the introduction of online journals (F(2,30) = 4.46, P < 0.02). CONCLUSIONS: The decrease in use of the print collection suggests that many patrons prefer to access journals online. The negative impact the online journals have had on the use of the journal titles available only in print suggests users may be compromising quality for convenience when selecting journal articles. Possible implications for collection development are discussed.

Analysis of Variance↗