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PubMed1988

Drinking under special occasion permits: a neglected aspect of alcohol control measures?

Drinking that occurs under special permits in Canada, such as at weddings, private receptions and parties, is a neglected aspect of alcohol research. These permits are issued for many different occasions and are most common in the provinces with the highest per capita alcohol consumption. Special permit drinking is becoming a social and alcohol-control issue but information on the problems it creates is sparse. Specific problem areas such as excessive drinking and drinking by minors have not been studied, but because such drinking is rarely monitored they may be substantial. Further study of special permit drinking is recommended.

Alcohol Drinking↗

PubMed1975

Demonstration of cytoplasmic processes in Millipore filters permitting kidney tubule induction.

The presence of cytoplasmic material inside thin Millipore filters between interacting mouse metanephric mesenchyme and spinal cord was investigated using different fixation methods. The transmission of induction was studied from sections of Zenker-fixed paraffin-embedded explants. Formation of kidney tubules was taken as evidence for induction. Filters with 0.8 and 0.22 mum pores permitted induction, whereas only 6 out of 31 filters with 0.1 mum pores did so. Glutaraldehyde-fixed Epon-embedded explants were used for study of cytoplasmic penetration into filters. In thick sections, filters with large pores were seen to contain cytoplasmic material at all levels. Filters with 0.1 mum pores usually showed only shallow ingrowth, but those which had permitted passage of induction contained material at least half way from the spinal cord and shallow ingrowth from the mesenchyme. With 0.8 mum filters the ingrowths from both sides met first after 18 h of transfilter cultivation. This has previously been shown to be the minimum time needed for induction to take place in this system. In electron microscopy cytoplasmic processes were seen deep inside the 0.8 and 0.22 mum filters regularly permitting induction. In small pores such material was only preserved by certain glutaraldehyde fixatives. Diffusion studies did not reveal major differences between induction-permitting 0.22 mum filters and induction-preventing 0.1 mum filters. Thus in the kidney tubule induction system this and our previous work speak in favor of a mechanism based on close apposition of cells rather than on long-range diffusion of inductive substances or on matrix interaction.

Animals↗

PubMedDate not supplied

Living with a large reduction in permited loading by using a hydrograph-controlled release scheme.

The Total Maximum Daily Load (TMDL) for ammonia and biochemical oxygen demand for the Pee Dee, Waccamaw, and Atlantic Intracoastal Waterway system near Myrtle Beach, South Carolina, mandated a 60-percent reduction in point-source loading. For waters with a naturally low background dissolved-oxygen concentrations, South Carolina anti-degradation rules in the water-quality regulations allows a permitted discharger a reduction of dissolved oxygen of 0.1 milligrams per liter (mg/L). This is known as the "0.1 rule." Permitted dischargers within this region of the State operate under the "0.1 rule" and cannot cause a cumulative impact greater than 0.1 mg/L on dissolved-oxygen concentrations. For municipal water-reclamation facilities to serve the rapidly growing resort and retirement community near Myrtle Beach, a variable loading scheme was developed to allow dischargers to utilize increased assimilative capacity during higher streamflow conditions while still meeting the requirements of a recently established TMDL. As part of the TMDL development, an extensive real-time data-collection network was established in the lower Waccamaw and Pee Dee River watershed where continuous measurements of streamflow, water level, dissolved oxygen, temperature, and specific conductance are collected. In addition, the dynamic BRANCH/BLTM models were calibrated and validated to simulate the water quality and tidal dynamics of the system. The assimilative capacities for various streamflows were also analyzed. The variable-loading scheme established total loadings for three streamflow levels. Model simulations show the results from the additional loading to be less than a 0. 1 mg/L reduction in dissolved oxygen. As part of the loading scheme, the real-time network was redesigned to monitor streamflow entering the study area and water-quality conditions in the location of dissolved-oxygen "sags." The study reveals how one group of permit holders used a variable-loading scheme to implement restrictive permit limits without experiencing prohibitive capital expenditures or initiating a lengthy appeals process.

Ammonia↗

PubMed1996

Capsid targeting sequence targets foreign proteins into bacteriophage T4 and permits proteolytic processing.

A membrane-independent morphogenetic viral signal peptide is identified within bacteriophage T4 internal protein III (IPIII). Utilizing a phagederived expression-packaging-processing system, which packages foreign proteins fused with IPIII into the phage capsid, a synthetic cleavage site introduced at the C terminus of IPIII, is demonstrated to be functional and permits processing of fusion proteins. IPIII, which possesses a native P21 cleavage site at its N terminus, is altered to possess a second P21 cleavage site at its C terminus where cleavage occurs by means of the scaffold proteinase P21 within the capsid. The altered IPIII was inserted into an expression vector to permit the creation of fusion proteins with staphylococcal nuclease, EcoRI endonuclease, beta-globin, and luciferase. Western immunoblot analysis of packaged T4eG326 indicates that the IPIII:fusion-proteins are packaged into phage and proteolytically processed, thus the synthetic P21 cleavage site positioned at the C terminus of IPIII is demonstrated to be functional, and 20 to 200 protein molecules are packaged per capsid. Truncation experiments identified the minimal portion of IPIII required to achieve targeting into the phage capsid as a ten amino acid residue from the N terminus, which includes the N-terminal methionine residue and the proteinase P21 cleavage site, designated the CTS (capsid targeting sequence). The addition of the CTS to a fragment of luciferase permits the protein to be packaged and processed, which demonstrates that the CTS is by itself sufficient to target foreign protein to the capsid. The imputed dual function of the CTS is supported by site-directed PCR mutagenesis, which reveals two functionally separate domains of the CTS for targeting and processing. The CTS appears to function in a core-related targeting mechanism that directs a polymorphic set of proteins into the T-even capsid or scaffold. Although structure formation is often assumed to involve extended protein interfaces, the analysis shows that a limited but specific sequence, the CTS, drives the interaction required to achieve targeting.

Amino Acid Sequence↗

PubMed1999

Validity of Performance Criteria and a Tentative Model for Regulatory Use in Compensatory Wetland Mitigation Permitting.

/ Wetland policy in the United States is guided by the goal of no net loss of wetland habitat and is enforced primarily through the permitting requirements instituted under the Clean Water Act, which regulates the waters of the United States. Under these regulations, a permit applicant may be required to provide compensatory mitigation or restoration when wetland loss cannot be avoided or minimized. The practice has been to measure gains and losses primarily in terms of functions and values, the assessment of whichhas been rendered difficult because the agencies operate under limitations of time and experience, and because there are large areas of uncertainty in wetland science itself.The purpose of this paper is to develop the principles for a manageable and practical set of performance criteria that will reasonably assure no net loss in a situation in which it cannot be absolutely assured. To this end, the performance criteria proposed for 116 compensatory wetland projects on file with the Army Corps of Engineers in San Francisco, between 1988 and 1995, were examined. The trends discerned in the project proposals were analyzed and evaluated in light of the current state of wetland science. Specific suggestions for the development of uniform criteria in each of four major wetland types-riparian, perennial tidal, perennial nontidal, and seasonal-are discussed, and a system of regulation tying qualitative assessment with quantitative requirements is outlined as a reasonable solution to the enforcement of the no-net-loss policy.KEY WORDS: Wetlands; Compensatory mitigation projects; 404 permits; Clean Water Act; Performance criteria, San Francisco, Californiahttp://link.springer-ny.com/link/service/journals/00267/bibs/24n3p327.html

Journal Article↗

PubMed2001

Optimisation of oil red O staining permits combination with immunofluorescence and automated quantification of lipids.

The objective of the present study was to develop a stain permitting automated quantification of myocellular lipid depositions in skeletal muscle sections together with immunolocalisation of other myocellular constituents by fluorescence microscopy. Lipid droplets were detected in skeletal muscle by oil red O (ORO). Conventional ORO was modified to diminish background staining, prevent crystallisation of ORO and to optimise lipid retention in cryosections. These modifications resulted in a punctate staining of lipid droplets, rather than the somewhat diffuse staining by conventional ORO. Small cavities in muscle sections (like the lumen of small blood vessels) lack ORO when using the protocol presented here. In addition a staining protocol is presented combining ORO with immunofluorescence. This combination permits multiple staining studies in the same section. Thus, lipid droplets can be studied together with immunolabelling of proteins involved in lipid handling and metabolism. This will extend our knowledge on the subcellular localisation of lipid handling proteins (i.e. enzymes and fatty acid transporting proteins) in relation to the localisation of lipid depositions. In conclusion, the protocol presented here permits examination of ORO-stained lipid droplets in skeletal muscle sections together with multiple staining of other immunodetectable proteins present in skeletal muscle by quantitative fluorescence microscopy.

Animals↗

PubMed1989

Glycerol, sodium phosphate, and sodium chloride permit the solubilization and partial purification of rat hepatic alpha 1-receptors by 3-(3-cholamidylpropyl)-dimethylammonio-1-propanesulfonate.

CHAPS [3-(3-cholamidylpropyl)-dimethylammonio-1-propanesulfonate], a zwitterionic detergent, has been used to solubilize the rat hepatic alpha 1-adrenergic receptor. Although the use of this detergent alone permitted a poor receptor solubilization, the inclusion of sodium phosphate, sodium chloride, and glycerol to the medium allowed 30% of the binding activity observed in plasma membranes to be recovered. Binding of the selective alpha 1-adrenergic antagonist, [3H]prazosin, by the solubilized preparation was saturable and of high affinity. In addition, binding of the radioligand was inhibited by a variety of adrenergic agents with affinity, specificity, and stereoselectivity comparable to that observed in plasma membranes. The use of glycerol in the solubilization medium permitted recovery of the solubilized receptor in a stable form (T1/2 = 72 h at 4 degrees C). Sequential affinity and size-exclusion gel chromatography allowed a 1000-fold purification of the solubilized receptor. The Stokes' radius and the apparent molecular mass of the purified receptor-Chaps complex (48.4 A and 160,000 Da, respectively), determined by gel filtration chromatography, were similar to those previously obtained for the rat hepatic alpha 1-receptor purified after solubilization with the nonionic detergent digitonin. These data indicate that the combination of Chaps, sodium phosphate, sodium chloride, and glycerol permitted the solubilization and partial purification of hepatic alpha 1-receptor in an active and stable form. The use of this technique might be useful for the solubilization of other membrane-bound proteins by Chaps whose biophysical characteristics make it an ideal detergent for reconstitution experiments.

Animals↗

PubMed1994

Two-stage selection of sequences from a random phage display library delineates both core residues and permitted structural range within an epitope.

Libraries of random peptides can be screened to identify species which interact with antibodies or receptors. Similarly, maps of native molecular interactions can frequently be deduced by screening a limited set of peptide fragments derived from sequences within a native antigen or ligand. However, the existence of cross-reactive sequences that mimic original epitopes and the limited replaceability of amino acid residues suggest that the sequence space accessible by a receptor can be much broader. Definition of this space is of particular importance where structural information is required for peptidomimetic or drug design. We have used a two-stage selection scheme to expand the sequence space accessible by a phage display library and to define peptide epitopes of the anti-FLAG octapeptide monoclonal M2 antibody. Affinity selection of a primary library of 2 x 10(6) random decapeptides identified a non-contiguous core of three residues in the binding motif Tyr-Lys-Xaa-Xaa-Asp. A second stage library with 2 x 10(7) individual clones bearing the core motif but with the remaining flanking and internal residues re-randomized permitted access to a broader sequence space represented in a library equivalent to several orders of magnitude larger. Data here demonstrate that extended access to binding sequence space permitted by multi-stage screening of phage display libraries can reveal not only essential residues required for ligand binding, but also the ligand structural range permitted within the receptor binding pocket.

Amino Acid Sequence↗

PubMed1998

Chronic treatment of female rhesus monkeys with low doses of the antiprogestin ZK 137 316: establishment of a regimen that permits normal menstrual cyclicity.

Large doses of antiprogestin typically disrupt menstrual cyclicity. A chronic low-dose regimen of the potent new antiprogestin ZK 137 316, which permits continued menstrual cyclicity but alters gonadal-reproductive tract activity, was established. Rhesus monkeys received vehicle (n = 6) or 0.01 (n = 8), 0.03 (n = 8) or 0.1 (n = 5) mg ZK 137 316/kg body weight daily for five menstrual cycles (C-1 to C-5). Oestradiol, progesterone and gonadotrophin profiles were normal during cycles involving vehicle and 0.01 and 0.03 mg ZK 137 316/kg body weight. In the 0.1 mg/kg group, mid-cycle oestradiol and gonadotrophin surges, and subsequent progesterone production, were absent in C-3 and C-5. Ovarian cyclicity was accompanied by timely menstruation in the vehicle and 0.01 mg/kg groups. By C-3, half the animals in the 0.03 mg/kg group and all animals in the 0.1 mg/kg group were amenorrhoeic. A corpus luteum was noted during the mid-luteal phase of C-5 in the vehicle, 0.01 mg/kg and 0.03 mg/kg groups. Large antral and cystic follicles were evident in the 0.1 mg/kg group. Thus, a daily treatment with 0.01 mg/kg ZK 136317 permitted normal menstrual cyclicity in macaques. While the daily administration of 0.03 mg/kg ZK 136 317 allowed ovarian cyclicity, menstruation was disrupted in some animals. Increasing the dose to 0.1 mg/kg antagonized pituitary function and resulted in anovulation and amenorrhoea. A chronic low-dose regimen of the antiprogestin ZK 137 316, which permits normal ovarian/menstrual cyclicity, has potential as a contraceptive in women.

Animals↗

PubMed2005

The level of evidence for permitting a qualified health claim: FDA's review of the evidence for selenium and cancer and vitamin E and heart disease.

Health claims are authorized for the labeling of foods when there is significant scientific agreement among qualified experts on the evidence for a relationship between a food or food component (substance) and a disease. Qualified health claims are permitted when there is less scientific evidence for a substance-disease relationship, therefore requiring qualifying language. The evidence for a relationship between vitamin E and heart disease and selenium and cancer was reviewed by the U.S. FDA. It was determined that there was insufficient evidence to permit a qualified health claim for vitamin E and cancer, whereas there was some evidence for permitting a qualified health claim for selenium and cancer. The rationale for these conclusions is discussed below.

Cardiovascular Diseases↗

PubMed2003

Should people who donate a kidney to a stranger be permitted to choose their recipients? Views of the United States public.

BACKGROUND: Several transplant centers have begun to accept kidneys donated by altruistic living strangers. Many of these centers insist that such donations be nondirected, meaning that the donors may not choose their recipients. On the other hand, some authors have argued that anonymous donors should be allowed to select their recipients. This study was designed to explore public attitudes toward this issue. METHODS: Two telephone surveys of US adults, each including more than 1000 participants, were conducted. The first asked about the general acceptability of allowing altruistic strangers to direct donations, the willingness to donate a kidney to a stranger, and the impact of permitting directed donation on willingness to give. The second survey asked about the acceptability of directed donation to members of specific groups. RESULTS: About one quarter of the respondents said they would donate a kidney to a stranger for free, and the vast majority of them would donate even if they could not choose their recipients. Two thirds would not allow anonymous kidney donors to direct their gifts to a member of a specific racial or religious group, but three quarters would support kidney donations directed to children. CONCLUSIONS: These data support the current policy of several transplant centers that people who wish to donate a kidney to a stranger are not permitted to choose their recipients on the basis of membership in a racial or religious group. On the other hand, theoretical considerations and the results of this study suggest that people who wish to donate a kidney only to an unknown child should be permitted to do so.

Adult↗

PubMed2006

Economic growth and biodiversity loss in an age of tradable permits.

Tradable permits are increasingly becoming part of environmental policy and conservation programs. The efficacy of tradable permit schemes in addressing the root cause of environmental decline-economic growth--will not be achieved unless the schemes cap economic activity based on ecological thresholds. Lessons can be learned from the largest tradable permit scheme to date, emissions trading now being implemented with the Kyoto Protocol. The Kyoto Protocol caps neither greenhouse gas emissions at a level that will achieve climate stability nor economic growth. If patterned after the Kyoto Protocol, cap-and-trade schemes for conservation will not ameliorate biodiversity loss either because they will not address economic growth. In response to these failures to cap economic growth, professional organizations concerned about biodiversity conservation should release position statements on economic growth and ecological thresholds. The statements can then be used by policy makers to infuse these positions into the local, national, and international environmental science-policy process when these schemes are being developed. Infusing language into the science-policy process that calls for capping economic activity based on ecological thresholds represents sound conservation science. Most importantly, position statements have a greater potential to ameliorate biodiversity loss if they are created and released than if this information remains within professional organizations because there is the potential for these ideas to be enacted into law and policy.

Air Pollution↗

PubMed1988

A suggested approach to overcome California's inability to permit urban resource recovery facilities.

The premature political termination of the permit reviews of five urban resource recovery projects in California may indicate a deficiency in the process under which project health risk assessments are performed and policy biases of the public agencies that control the contents of the assessments. This article examines the permitting process that separates project health risk assessments from environmental impact reports and the anti-resource-recovery goals and policies of the state and local agencies that guide the preparation of project health risk assessments. Also examined are two independent studies made of the informational adequacy of the LANCER project documents. The article concludes that it is only by merging the risk assessment process with the environmental review process that control of the agencies can be loosened and project proponents be able to prepare project risk assessments for urban California resource recovery projects that will offer the projects any likelihood of being able to complete the permitting process.

Air Pollution↗

PubMed1998

Stent dilation of superior vena cava and innominate vein obstructions permits transvenous pacing lead implantation.

The purpose of this study was to assess the feasibility of stent dilation of venous obstructions/occlusions to permit transvenous pacing lead implantation. Innominate vein or superior vena cava (SVG) obstruction may preclude the implantation of transvenous pacing leads. Patients with d-transposition of the great arteries, after a Mustard or Senning procedure, and children with previously placed transvenous pacing leads are at higher risk for this vascular complication. From May 1993 to January 1996, eight pediatric patients who underwent transvenous pacing lead implantation or replacement were found to have significant innominate vein or SVC obstruction or occlusion. Utilizing intravascular stents, a combined interventional and electrophysiological approach was used to relieve the venous obstruction and to permit implantation of a new transvenous pacing lead. Two patients had complete SVC occlusion requiring puncture through the obstruction with a transseptal needle. Vessel recanalization was achieved with balloon dilation and stent implantation. The remaining six patients had severe venous obstruction with a mean minimum diameter of 3.1 +/- 3.3 mm. The mean pressure gradient across the obstructed veins was 8.6 +/- 7.3 mmHg. Following implantation of 15 Palmaz P308 stents in eight vessels, the mean diameter increased to 14.2 +/- 1.9 mm and the mean pressure gradient across the stented vessels decreased to 1.0 +/- 2.0 mmHg. A transvenous pacing lead was implanted successfully through the stent(s) immediately or 6-8 weeks later. Innominate vein and SVC obstruction can be safely and effectively relieved with intravascular stents and permit immediate or subsequent transvenous pacing lead implantation.

Adolescent↗

PubMed1988

Site in the cat-86 regulatory leader that permits amicetin to induce expression of the gene.

Expression of the plasmid gene cat-86 is induced in Bacillus subtilis by two antibiotics, chloramphenicol and the nucleoside antibiotic amicetin. We proposed that induction by either drug causes the destabilization of a stem-loop structure in cat-86 mRNA that sequesters the ribosome-binding site for the cat coding sequence. The destabilization event frees the ribosome-binding site, permitting the initiation of translation of cat-86 mRNA. cat-86 induction is due to the stalling of a ribosome in a leader region of cat-86 mRNA, which is located 5' to the RNA stem-loop structure. A stalled ribosome that is active in cat-86 induction has its aminoacyl site occupied by leader codon 6. To test the hypothesis that a leader site 5' to codon 6 permits a ribosome to stall in the presence of an inducing antibiotic, we inserted an extra codon between leader codons 5 and 6. This insertion blocked induction, which was then restored by the deletion of leader codon 6. Thus, induction seems to require the maintenance of a precise spatial relationship between an upstream leader site(s) and leader codon 6. Mutations in the ribosome-binding site for the cat-86 leader, RBS-2, which decreased its strength of binding to 16S rRNA, prevented induction. In contrast, mutations that significantly altered the sequence of RBS-2 but increased its strength of binding to 16S rRNA did not block induction by either chloramphenicol or amicetin. We therefore suspected that the proposed leader site that permitted drug-mediated stalling was located between RBS-2 and leader codon 6. This region of the cat-86 leader contains an eight-nucleotide sequence (conserved region I) that is largely conserved among all known cat leaders. The codon immediately 5' to conserved region I differs, however, between amicetin-inducible and amicetin-noninducible cat genes. In amicetin-inducible cat genes such as cat-86, the codon 5' to conserved region I is a valine codon, GTG. The same codon in amicetin-noninducible cat genes is a lysine codon, either AAA or AAG. When the GTG codon immediately 5' to conserved region I in cat-86 was changed to AAA, amicetin was no longer active in cat-86 induction, but chloramphenicol induction was unaffected by the mutation. The potential role of the GTG codon in amicetin induction is discussed.

Acetyltransferases↗

PubMed1993

Permit-required confined spaces.

The focus of the new Occupational Safety and Health Administration Standard: Permit-Required Confined Spaces (29 CFR 1910.146) is to protect workers who enter and work in areas with immediate health or safety risk from toxic, explosive, and/or asphyxiating atmospheres. The rule mandates that employers identify all permit-required confined spaces in their workplaces, prevent unauthorized entry into them, and develop a program to protect workers during authorized entry. Included in this standard are requirements for the content, issuance, and retention of entry permits; the provision of standby attendants; arrangements for rescue and other emergency services; the communication of hazards to workers (including contractors' employees); and appropriate training and equipment for authorized entrants, attendants, and entry supervisors.

Air↗

PubMed1980

Environmental Protection Agency--Hazardous waste and consolidated permit regulations, Parts X-XI. Final rule.

This rule establishes consolidated permit program requirements governing the Hazardous Waste Management program under the Resource Conservation and Recovery Act (RCRA), the Underground Injection Control (UIC) program under the Safe Drinking Water Act (SDWA), the National Pollutant Discharge Elimination System (NPDES) program and State Dredge on Fill ("404") programs under the Clean Water Act (CWA), and the Prevention of Significant Deterioration (PSD) program under the Clean Air Act, for three primary purposes: (1) To consolidate program requirements for the RCRA and UIC programs with those already established for the NPDES program. (2) To establish requirements for State programs under the RCRA, UIC, and Section 404 programs. (3) To consolidate permit issuance procedures for EPA-issued Prevention of Significant Deterioration permits under the Clean Air Act with those for the RCRA, UIC, and NPDES programs.

Government Agencies↗

PubMed2004

Paradoxical REM sleep promoting and permitting neuronal networks.

Since its electrophysiological identification in the 1950's, the state of REMS or PS has been shown through multiple lines of evidence to be generated by neurons in the oral pontine tegmentum. The perpetration of this paradoxical state that combines cortical activation with the most profound behavioral sleep occurs through interplay between PS-promoting (On) and PS-permitting (Off) cell groups in the pons. Cholinergic cells in the LDTg and PPTg promote PS by initiating processes of both forebrain activation and peripheral muscle atonia. Bearing alpha1-adrenergic receptors, cholinergic cells, which likely project to the forebrain, are excited by NA and active during both W and PS (W/PS-On), when they promote cortical activation. Bearing alpha2-adrenergic receptors, other cholinergic cells, which likely project to the brainstem, are inhibited by NA and thus active selectively during PS (PS-On), when they promote muscle atonia. Noradrenergic, together with serotonergic, neurons, as PS-Off neurons, thus permit PS in part by lifting their inhibition upon the cholinergic PS-On cells. The noradrenergic/serotonergic neurons are inhibited in turn by local GABAergic PS-promoting neurons that may be excited by ACh. Other similarly modulated GABAergic neurons located through the brainstem reticular formation become active to participate in the inhibition of reticulo-spinal and raphe-spinal neurons as well as in the direct inhibition of motor neurons. In contrast, a select group of GABAergic neurons located in the oral pontine reticular formation and possibly inhibited by ACh turn off during PS. These GABAergic PS-permitting neurons release from inhibition the neighboring large glutamatergic neurons of the oral pontine reticular formation, which are likely concomitantly excited by ACh. In tandem with the cholinergic neurons, these glutamatergic reticular neurons propagate the paradoxical forebrain activation and peripheral inactivation that characterize PS.

Animals↗