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PubMed2004

Community-based interventions for the prevention of burns and scalds in children.

BACKGROUND: Burns and scalds are a significant cause of morbidity and mortality in children. Successful counter-measures to prevent burn and scald-related injury have been identified. However, evidence indicating the successful roll-out of these counter-measures into the wider community is lacking. Community-based interventions in the form of multi-strategy, multi-focused programmes are hypothesised to result in a reduction in population-wide injury rates. This review tests this hypothesis with regards to burn and scald injury in children. OBJECTIVES: To assess the effectiveness of community-based interventions, defined as coordinated, multi-strategy initiatives, for reducing burns and scalds in children in children aged 0-14 years. SEARCH STRATEGY: The search strategy was based on electronic searches, handsearches of selected journals and snowballing from reference lists of selected publications. SELECTION CRITERIA: Studies were independently screened for inclusion by two reviewers. Included studies were those that reported changes in medically attended burn and scald-related injury rates in a paediatric population (age 0 - 14 years), following the implementation of a controlled community-based intervention. DATA COLLECTION AND ANALYSIS: Data were independently extracted by two reviewers. Meta-analysis was not appropriate, due to the heterogeneity of the included studies. MAIN RESULTS: Of 32 identified studies, only three met the criteria for inclusion. Only one of these three studies showed a significant decrease in paediatric burn and scald injury in the intervention community compared with the control community. The failure of the other two studies to show a positive result may have been due to limited time-frame for the intervention and/or failure to adequately implement the counter-measures in the communities. REVIEWERS' CONCLUSIONS: There are a very limited number of research studies allowing conclusions to be drawn about the effectiveness of community-based injury prevention programmes to prevent burns and scalds in children. There is a pressing need to evaluate high-quality community-based intervention programmes based on efficacious counter-measures to reduce burns and scalds in children. It is important that a framework for considering the problem of burns/scalds in children from a prevention perspective be articulated, and that an evidence-based suite of interventions be combined to create programme guidelines suitable for implementation in communities throughout the world.

Accident Prevention↗

PubMed1995

Thirty-five years of paediatric scalds: are lessons being learned?

All paediatric scalds admitted in 1991 to the Welsh Centre for Burns and Plastic Surgery have been analysed retrospectively and the results compared with similar studies from this centre reported in 1956 and 1984. This data was compared with similar reports from 6 other world-wide centres. Despite a modest population increase in Wales, the number of admissions has increased almost 50% in 35 years. Boys are more commonly affected and up to 67% of scalds occur in children under 2 years of age. The number of scalds from teapots has fallen from 20.8% to 2.7%, in contrast to scalds from cups of hot liquid which have increased from 8.9% to 42.5% over the 35-year period. Scalds from hot baths are rare in Holland and Denmark, in contrast to a 15.8% incidence in our centre. Hot kettles are a common cause of scalds, accounting for up to 28% of scalds in all the series reviewed. The incidence of this injury has not decreased over the last 35 years. Recommendations are made about improved health and safety education for parents, the use of coiled kettle flexes, the temperature of stored water which should not exceed 60 degrees C, and the fitting of thermostatic mixer taps to new houses and institutions, in an effort to decrease the incidence of paediatric scalds.

Accident Prevention↗

PubMed1998

Contribution of the sensory and sympathetic nervous system to scalding-induced edema in the rat paw.

It has recently been hypothesized that both the sensory and sympathetic nervous system contribute to the inflammatory reaction. A scalding model was developed in anaesthetized rats to investigate the contribution of neuropeptides in heat-induced edema localized to the hindpaw. After immersing the paw in water at 60 degrees C for 10, 20, 30 and 60 s, edemic reactions were registered as change of paw volume in a plethysmograph and hindpaw perfusates collected to measure the content of neuropeptides by radioimmunoassay. A scalding period of 30 s induced the most prominent edemic reaction. There was a marked increase of the sensory neuropeptide neurokinin A and the sympathetic related transmitter neuropeptide Y in hindpaw perfusates after scalding. The effect of peripheral nerve ligation on edemic reaction and on the release of neuropeptides was investigated in rats scalded for 30 s at 60 degrees C. There was a significant decrease of edema formation in the scalded nerve ligated paw as compared with the scalded paw on the non-ligated side. Neurokinin A was not detected in nerve ligated rats before or after scalding, whereas mononeuropathic rats showed increased concentrations of neuropeptide Y. The present results indicate that the sensory as well as the sympathetic nervous system, possibly through the release of neuropeptides, may contribute to scald-induced edema.

Animals↗

PubMed2003

Characterization of mouse short-chain aldehyde reductase (SCALD), an enzyme regulated by sterol regulatory element-binding proteins.

Sterol regulatory element-binding proteins (SREBPs) enhance transcription of genes encoding all of the proteins required for the cellular synthesis and uptake of cholesterol and unsaturated fatty acids. Here, we use suppression subtractive hybridization to identify a previously unrecognized SREBP-enhanced gene in mice. The gene encodes a membrane-bound enzyme that we designate SCALD, for short-chain aldehyde reductase. We expressed SCALD in bacteria, purified it extensively, and studied its catalytic properties in detergent solution. The enzyme specifically uses NADPH to reduce a variety of short-chain aldehydes, including nonanal and 4-hydroxy-2-nonenal. The enzyme also reduces retinaldehydes, showing equal activity for all-trans-retinal and 9-cis-retinal. Northern blot analysis indicates that SCALD is expressed most abundantly in mouse liver and testis. In the liver of mice, SCALD is suppressed by fasting and induced by refeeding, consistent with regulation by SREBPs. In testis, SCALD expression is restricted to pachytene spermatocytes, as revealed by visualization of mRNA and protein. SCALD is also expressed in four layers of the retina, including the outer segment of rods and cones, as revealed by immunohistochemistry. SCALD appears to be the mouse ortholog of the human protein that has been designated variously as prostate short-chain dehydrogenase/reductase 1, retinal reductase 1, and retinol dehydrogenase 11. In view of its ability to reduce short-chain aldehydes in addition to retinals, we propose that SCALD may be induced by SREBP in liver and other tissues to prevent toxicity from fatty aldehydes that are generated from oxidation of unsaturated fatty acids that are synthesized as a result of SREBP activity.

Aldehyde Reductase↗

PubMed1994

Patterns of scald injuries.

OBJECTIVES: To describe common patterns of bath water scald injuries in children, to examine differences between accidental and non-accidental bath water scalds in children, and to examine potential for prevention. DESIGN: A two year six month retrospective analysis of admissions to a specialist burns unit. SETTING: The Burns Unit, St Lawrence Hospital, Chepstow serving children from south and west Wales. SUBJECTS: Sixty eight children attending the Burns Unit for treatment of bath related scald injuries. RESULTS: Bath scalds in children under 5 years of age was the cause of 14.7 per 100,000 children being admitted to the specialist burns unit in a year. The majority of the children were injured by falling in the bath but the tap was turned on by seven children themselves and by 10 siblings. Six children put hands in the hot water and two children were accidentally put into bath water that was too hot and were quickly withdrawn. Four children suffered probably non-accidental immersion scald injuries from hot water. They were characterised by a clear tide mark, a story that did not fit the injuries, associated injuries, and by symmetrical lesions. Accidental scalds were irregular geographical injuries and were asymmetrical. CONCLUSIONS: Bath scalds are a significant problem in children under 5 years. Their prevention should be part of an injury control programme on a local and national level. The best way to achieve this would be by reducing the temperature in domestic hot water tanks. The recognition of non-accidental bath scalds can be assisted by the pointers outlined and should be done in a multidisciplinary way with plastic surgeons, paediatricians, and social workers working together.

Accidents, Home↗

PubMed2005

Kitchen scalds and thermal burns in children five years and younger.

OBJECTIVE: The current study was undertaken to describe patterns of kitchen burns and scalds to young children to understand better why such injuries continue to occur despite intervention efforts. METHODS: Emergency department-treated thermal burns and scalds associated with nonelectric cookware were examined from a national sample, collected by the US Consumer Product Safety Commission's injury surveillance system over a 6-year period, 1997-2002. Data extracted from the cases included age, gender, body part, disposition, case weight, causal substance, and injury pattern. Data were analyzed using Epi Info 2002, with significance assessed by chi(2) test. RESULTS: Scalds were approximately twice as common as were thermal burns. Hot water was the chief causal agent for scalds. The 2 most common scald injury patterns were (1) the child reached up and pulled a pot of hot water off the stove or other elevated surface and (2) the child grabbed, overturned, or spilled a container of hot water onto him- or herself. One-year-olds were at highest risk for scalds and thermal burns. Scalds resulted in significantly more hospitalizations than did thermal burns. In nearly all injury patterns, more boys than girls were injured, but the ratio varied depending on the injury pattern. CONCLUSIONS: Although the kitchen is recognized as a room that is hazardous for young children, parents seem not to recognize or anticipate the risk for burns and scalds. The ability of children, especially toddlers, to reach containers of hot liquids on elevated surfaces is reflected in the injury data and is explained by anthropometry data, yet there is an apparent failure on the part of parents to recognize children's ability to gain access to the hazard and a failure to recognize the potential severity of resulting injury. These failures might explain why behavioral interventions (eg, place pots on back burners of stove) have been nonmotivating and ineffective. A multifaceted spectrum of prevention that has individual, community, and organizational components may prove to be more useful.

Accidents, Home↗

PubMed2000

[The effect of Shenmai injection on the expression of TNFalpha mRNA of the macrophages in scalded mice].

OBJECTIVE: To explore the effect of Shenmai injection on the expression of TNFalpha mRNA of peritoneal macrophages in scalded mice. METHODS: BALB/c mice inflicted with 11% TBSA III degree scalding injury were employed as the model. Shenmai injection was composed of 100 mg of red ginseng and 100 mg of lily turf root in 1 ml injection solution and was administered to mice via peritoneal injection in a dose of 2.5 ml per kilogram of mice body weight per day for 5 days. The expression of TNFalpha mRNA was determined by semi-quantitative RT-PCR. RESULTS: The expression of macrophage TNFalpha mRNA was significantly increased in the scalded mice, while the expression was obviously lowered after the administration of Shenmai injection (P < 0.01) to scalded mice and the survival rate of the scalded mice was thus markedly increased (P < 0.05). CONCLUSION: The beneficial effect of Shenmai injection on the scalded mice might be related to its inhibitory effect on the hyperexpression of macrophage TNFalpha mRNA. Shenmai injection could lower down the expression of TNFalpha in scalded mice and raise the survival rate of scalded mice.

Animals↗

PubMed2003

[Separation and identification of down-regulated proteomics of intestinal mucosa in scalded rats].

OBJECTIVE: To explore the pathogenesis of postburn intestinal mucosal injury in scalded rats. METHODS: The rats inflicted with full thickness burn were employed as the model. The two-dimensional electrophoresis (2-DE) was employed to identify the down-regulating proteins from the differential proteins in scalded rats. Spot detection and matching were performed with Image Master 2D Elite. Mass spectrometry was performed on Bruker BIFLEX III TOF. RESULTS: There are 34 points of proteins in intestinal mucosa in scalded rats which were down-regulated at 6 and 12 postburn hours. Among them 22 proteins were employed for the identification and analysis. Mitochondrial aconitase, alpha-propionyl -CoA carboxylase heavy chain A of F1-ATPase in rat liver, Troponin short-chain of hydroxyacyl-coenzyme A dehydrogenase, alpha-subunit of P-electronic transferring flavoprotein (ETF) were down-regulating proteins correlated with mitochondria in intestinal mucosa in severely scalded rats. Triosephosphate isomerase 1 and cytosolic epoxide hydrolase were down-regulating proteins participated in metabolism of scalded rats. Fibromodulin, dynein-like protein, Troponin-2 and myosin light chain 3 alkali (MLC) were down-regulating proteins correlated with cellular skeletal protein. Glucocorticoid-inducible protein, nuclear factor1-B2, BRCA1, transcriptive factor EB (estradiol benzoate), beta2 subunit of G-protein, N-methyl-D-aspartate receptor1 (NMDAR) were down-regulating proteins participated in postburn regulation of intestinal mucosa in scalded rats. T-cell receptor-V-delta 6 and Ig heavy chain V region protein 1 were down-regulating proteins correlated with the immunomodulation of intestinal mucosa in scalded rats. CONCLUSION: The down-regulating proteins of intestinal mucosa in scalded rats exhibited close relationship with mitochondria.

Animals↗

PubMed2006

[Effects of Xuebijing injection on multiple organ dysfunction and mortality in rats with delayed resuscitation for scald injury].

OBJECTIVE: To investigate the effects of Xuebijing injection on multiple organ dysfunction and mortality in rats with delayed resuscitation after scald injury. METHODS: Wistar rats were subjected to a 30% total body surface area (TBSA) full-thickness scald injury in the experimental groups. One hundred and thirty male Wistar rats were randomly divided into sham scald group (n=10), thermal injury group (n=60, 40 ml/kg normal saline was infused peritoneally 6 hours postburn), and Xuebijing treatment group (n=60, 4 ml/kg Xuebijing injection was injected twice a day in addition to delayed resuscitation). Animals of the thermal injury group and Xuebijing treatment group were re-divided into three subgroups according to the different time points of treatment: 2 hours before scald (n=20), 2 hours after scald (n=20), and 12 hours after scald (n=20). Multiple organ function parameters and 7-day mortality were determined in all the groups. RESULTS: The mortality of animals with Xuebijing treatment at 12 hours postburn was significantly lower than that of scald controls (75.0% vs. 40.0%, P<0.05). In comparison with scald group, the animals in Xuebijing treatment group were found to have significantly decreased values of organ functional parameters at 12 hours postburn, including serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine (Cr) and creatine kinase (CK, P<0.05 or P<0.01). CONCLUSION: These findings indicate that Xuebijing injection could obviously lower the mortality of rats with delayed resuscitation after burn injury, and it markedly protects against multiple organ dysfunction secondary to severe burns.

Animals↗

PubMed1987

Effect of single and repeated scalding on adenine nucleotides concentration in rat liver.

Changes in ATP, ADP, AMP, and total adenine nucleotide (TAN) concentrations and in the adenylate energy charge (EC) were investigated in the livers of rats subjected to single and repeated scalding. Single scaldings were of two grades of severity: 20% (nonlethal) and 40% (lethal within 24 hr) of the total body surface area. A repeated scald (additional 20%) was inflicted on the intact skin of the opposite side of the body either 3 hr or 3 days after a nonlethal scald. The results suggest that the energy state of the liver is related to the severity of a single scald, that the EC at the moment of repeating the scald is important for survival, and that the changes in ATP, EC, and TAN following a repeated scald are qualitatively or quantitatively different from those after a single scald.

Adenine Nucleotides↗

PubMed2005

Recovery of bacteria from broiler carcass respiratory tracts before and after immersion scalding.

Occlusion of the trachea and decapitation were compared with a conventional unilateral neck cut for effects on bacteria entering into the respiratory tract of broiler carcasses during scalding. In experiment 1, the trachea was occluded prior to the carcass entering the scalder to determine if bacterial recovery from the respiratory tract could be diminished. The first carcass was removed at the end of bleeding, and a plastic cable tie was placed around the neck of a second carcass and tightened to occlude the trachea. After proceeding through the triple-tank immersion scalder, the second carcass (trachea occluded) was removed, and a third carcass (without the trachea occluded during scalding) was removed. In experiment 2, after being stunned, carcasses were unilaterally bled or decapitated. Unilaterally bled and decapitated carcasses were removed at the end of bleeding and after scalding. In both experiments, trachea were cannulated, and respiratory tract rinses were collected. For experiment 1, the numbers of bacteria recovered (log10 cfu/mL of rinse) from prescald nonoccluded carcass respiratory tract rinses were 2.5 Escherichia coli, 2.6 coliforms, and 3.2 total aerobes. Respiratory tract rinses from carcasses sampled postscald (without occluding the trachea) had higher bacteria numbers at 4.6 E. coli, 5.0 coliforms, and 5.4 total aerobes. Respiratory tract rinses from carcasses with the trachea occluded prior to scalding had the lowest number of bacteria at 1.9 E. coli, 2.3 coliforms, and 2.7 total aerobes. In experiment 2, the numbers of bacteria recovered from respiratory tract rinses of unilaterally bled or decapitated carcasses did not differ prescald or postscald, although all postscald values were higher (P < 0.05). Results confirmed that bacteria numbers increased within the respiratory tract during immersion scalding, the increase could have been prevented by occluding the trachea prior to scalding, and decapitation did not alter the number of bacteria recovered from respiratory tract rinses prior to or following immersion scalding.

Animals↗

PubMedDate not supplied

Results of a focused scald-prevention program.

Scalds are a leading cause of burn injury for young children. A focused prevention program was developed in the zip code accounting for the majority of scald burns. This study investigated the effect of the program. Families in the high-risk area were identified at clinics, community centers, and schools. Parent workshops and home visits were the interventions used. A pretest was administered at the workshop to measure baseline knowledge. A post-test was administered at either the home visit or by telephone to measure change in knowledge. A survey was used to measure baseline scald risks in the home. Home visits were used to reinforce information from workshops, evaluate the home environment, and assist parents to make environmental changes. Changes to the home environments were made, with antiscald devices installed in the shower, sink, or bathtub depending on parent preference. The survey was repeated on a follow-up home visit to determine whether parents adhered to environmental changes and safety practices. The postmeasurements were performed from 6 to 12 months after the initial measurement. More than 900 parents attended the initial workshops, and 173 consented to participate in the follow-up study and took the pretest. Of these, 62 completed the post-test, and 48 participated in a home visit. The mean pretest score was 72 +/- 1%, and mean post-test score was 85 +/- 1% (P < .01). The initial home visit surveys revealed an average of 7 +/- 2 scald risks per household, whereas follow-up surveys showed an average of 2 +/- 1 risks (P < .01). Antiscald devices were installed in 37 households on the initial visit and remained in place and functioning in 22 households (60%) on the follow-up visit. Before the focused prevention program, the admission rate from the target zip code was 137 per 100,000 children ages 0 to 5 years. After the intervention, there was a greater than 2-fold reduction, to 59 per 100,000 (P < .01). In addition, there were no new scald burns in the homes in which the focused prevention program took place. This study demonstrates that a focused burn-prevention program can identify high-risk groups, decrease the number of scald risks per home, and decrease the rate of scald burns in the population. This straightforward program could be used to intervene in high-risk groups in other communities.

Accidents, Home↗

PubMed1972

Steam versus hot-water scalding in reducing bacterial loads on the skin of commercially processed poultry.

A comparison of two types of scalders was conducted to determine their effectiveness in reducing bacterial contamination of poultry carcasses. A conventional hot-water scalder and a prototype model of a steam scalder were tested under commercial conditions. Total plate counts from steam-scalded birds were significantly lower than the counts of water-scalded birds immediately after scalding and again after picking. No differences in the two methods could be found after chilling. Coliform counts from steam-scalded birds were significantly lower than the counts from water-scalded birds immediately after scalding. No significant differences in coliform counts were detected when the two scald methods were compared after defeathering and chilling.

Bacteria↗

PubMed1999

"First aid for scalds" campaign: reaching Sydney's Chinese, Vietnamese, and Arabic speaking communities.

OBJECTIVES: As a serious yet preventable problem, scald injuries in children have been a priority for prevention in Australia and other developed countries. Not only can the occurrence of scalds be prevented, but immediate first aid treatment offers an effective method for secondary prevention, reducing the severity of scalds. Despite the success of scald prevention initiatives, local evidence suggested that first aid knowledge was lacking in some minority ethnic groups. To redress this gap, the "First Aid for Scalds" campaign for those from a non-English speaking background was specifically targeted to three ethnic groups (Vietnamese, Chinese, and Arabic), with the aim of increasing the proportions of parents and caregivers who had correct knowledge of first aid treatment for scalds. The primary strategy was a media campaign, including advertisements on ethnic radio and in ethnic newspapers. METHODS: The evaluation design included formative research and impact evaluation. The impact evaluation study involved random population based telephone surveys with each of the three language groups, before and after the campaign, to assess the reach and effectiveness of the campaign. RESULTS: After the campaign, there were significant increases in the proportion of people who knew the correct first aid treatment for scalds. There were substantial variations in campaign recall and knowledge between each of the three language groups. The largest improvement was found in the Vietnamese group. CONCLUSION: The association between campaign recall and increase in correct knowledge, and the absence of any similar interventions during the campaign period, give credence to the conclusion that the changes observed were a result of the campaign. The results demonstrate the value of community based injury prevention campaigns specifically targeting linguistically diverse communities.

Accident Prevention↗

PubMed1984

A morphological and histochemical study of the scalded hairy mouse ear.

The right ear of mice was scalded at 51 degrees C and 53 degrees C for 20 s, using the recently developed dip burn model, and examined morphologically and histochemically at intervals for 2 h postburn. The left, unburned ear served as control. Burned ears intended for light and electron microscopy were fixed in situ by means of vascular perfusion. Ears for histochemical studies were cut off and immediately frozen at -140 degrees C. The enzyme histochemical analyses comprised a variety of oxido-reductases and hydrolases. After a 51 degrees C scald, scattered epidermal cell damage was observed with intracellular edema and mitochondrial destruction. Most microvessels were patent. Scalding with 53 degrees C water produced extensive cellular changes in the epidermis from 5 min including mitochondrial alterations, tonofilament aggregation and intercellular separations, progressing into complete epidermal destruction and lysis at 2 h postburn. Morphologically, many microvessels were occluded by cellular aggregates or debris after endothelial cell destruction. Of the histochemical assays the test for succinic dehydrogenase gave conclusive results that correlated well with the morphological findings, indicating partial dysfunction of epidermal cells 2 h postburn after a 51 degrees C scald and early, almost total absence of activity from 5 min postburn after a 53 degrees C scald injury. Scalding at 53 degrees C invariably leads to ear necrosis. This might be explained by the epidermal destruction, which in turn seems related to mitochondrial viability, and microcirculatory blood flow. The sensitivity of this experimental model precludes direct comparisons with studies of scalds to skin covering large tissue volumes.

Animals↗

PubMed1999

[Effect of different immunomodulators on macrophage function in rats with scald injury].

OBJECTIVE: To explore effect of different immunomodulators on macrophage (M phi) functions in rats with scald injury. METHODS: The M phi functions in various groups of rats with scald on the 5th and 10th day after scald were respectively determined with methods of McAb APAAP, agar bacteriolytic plate and MTT colorimetry. RESULTS: The results showed that: 1. after scald, presenting rate of Ia antigen, ability of antigen presentation, phagocytic power to candida albicans and lysozyme ability of M phi were significantly decreased, the capacity of M phi secreting TNF was markedly increased compared with those of the normal group, and the differences were significant (P < 0.01); 2. after the therapy with immunodulators, the above functions of M phi in rats with scald improved markedly. As compared with those of the control group, differences were significant (P < 0.01). CONCLUSION: The specific immune RNA, which is administered by intraperitoneal injection early after scald, can markedly improve immune functions of rats with scald.

Adjuvants, Immunologic↗

PubMed2002

[The modulating effects of transfected IkappaBalpha gene on the NF-kappaB activity of hepatic tissue in scalded rats].

OBJECTIVE: To explore the modulating effects mediated by recombinant IkappaBalpha gene by adenovirus vector transfection on the NF-kappaB activity of hepatic tissue in scalded rats. METHODS: After being primed by recombinant defect adenovirus (AdIkappaBalpha) vector, the rats were scalded. The hepatic tissue was harvested at different time points and the nuclear protein was extracted and reacted with [r(-23) P] ATP labelled NF-kB specific probe. NF-Kb/DNA combining activity was determined with electrophoretic mobility shifty assay (EMSA). The total RNA in hepatic tissue was extracted and the expressions of IL-1beta and TNFalpha mRNA were determined with RT-PCR. RESULTS: When compared to those in normal control, the NF-kappaB/DNA combining activity increased significantly in scalded rats at half an hour after scalding, and it lasted for 24 PBDs with rather strong activity. But in AdIkappaBalpha priming group, the rat NF-kappaB/DNA binding activity was slightly higher than that of normal control group, but was obviously lower than that of scalding group. CONCLUSION: The intracellular NF-kappaB in hepatic tissue was activated rapidly after that the rats were severely scalded, and the expression of IL-1beta and TNFalpha was enhanced significantly simultaneously. Priming of AdIkappaBalpha could evidently inhibit the activation of hepatic tissue NF-kB in rats injured by severe scalding, and down-regulated the expressions of IL-1beta and TNFalpha.

Adenoviridae↗

PubMed2005

[Influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats].

OBJECTIVE: To investigate the influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats, so as to provide a theoretical basis for the clinical use of hyperoxic fluid. METHODS: Sprague-Dawley (SD) rats with 35% TBSA full-thickness scald were randomly divided into scald control (S, n = 6,with intraperitoneal fluid infusion after scalding), hyperoxic (H, n = 6, with hyperoxic fluid infusion after scalding) groups. Six rats without scald injury served as normal group. The proteins in the intestinal mucosa were separated with the two-dimensional electrophoresis (2-DE), and were analyzed with ImageMaster 2D Elite. The influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats was studied with bio-spectrum, protein bank and document analysis. RESULTS: (1) Among the 34 down-regulated protein spots in S group, 9 of them definitely exhibited up-regulation compared with those in H group. (2) The expression of mitochondrial aconitase, alpha-propionyl-CoA carboxylase, short chain of hydroxyacyl-Coenzyme A dehydrogenase, transcription factor EB (estradiol benzoate), triosephosphate isomerase 1, T cell receptor V delta 6, and dynein-like protein-5 in H group were significantly up-regulated. CONCLUSION: The hyperoxic fluid could up-regulate the down-regulated proteins in rat intestinal mucosa at early postburn stage, so that the barrier function of intestinal mucosa of rats with severe burns could be partially recovered.

Animals↗