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International expert workshop on the analysis of the economic and public health impacts of air pollution: workshop summary.

Forty-nine experts from 18 industrial and developing countries met on 6 September 2001 in Garmisch-Partenkirchen, Germany, to discuss the economic and public health impacts of air pollution, particularly with respect to assessing the public health benefits from technologies and policies that reduce greenhouse gas (GHG) emissions. Such measures would provide immediate public health benefits, such as reduced premature mortality and chronic morbidity, through improved local air quality. These mitigation strategies also allow long-term goals--for example, reducing the buildup of GHG emissions--to be achieved alongside short-term aims, such as immediate improvements in air quality, and therefore benefits to public health. The workshop aimed to foster research partnerships by improving collaboration and communication among various agencies and researchers; providing a forum for presentations by sponsoring agencies and researchers regarding research efforts and agency activities; identifying key issues, knowledge gaps, methodological shortcomings, and research needs; and recommending activities and initiatives for research, collaboration, and communication. This workshop summary briefly describes presentations made by workshop participants and the conclusions of three separate working groups: economics, benefits transfer, and policy; indoor air quality issues and susceptible populations; and development and transfer of dose-response relationships and exposure models in developing countries. Several common themes emerged from the working group sessions and subsequent discussion. Key recommendations include the need for improved communication and extended collaboration, guidance and support for researchers, advances in methods, and resource support for data collection, assessment, and research.

Air Pollutants↗

Report from the 1st International NOD Mouse T-Cell Workshop and the follow-up mini-workshop.

A workshop on autoreactive T-cell responses in NOD mice was held to optimize autoreactive T-cell detection methodologies. Using different proliferation assay protocols, 1 of the 11 participating laboratories detected spontaneous T-cell responses to GAD(524-543) and insulin(9-23) in their NOD mice. Two other laboratories were able to detect autoreactive responses when using enzyme-linked immunospot assay (ELISPOT) and enzyme-linked immunosorbent assay (ELISA) analysis of cytokines in culture supernatants, suggesting that these assays provided greater sensitivity. To address the divergent findings, a follow-up mini-workshop tested NOD mice from four different colonies side-by-side for T-cell proliferative responses to an expanded panel of autoantigens, using the protocol that had enabled detection of responses in the 1st International NOD Mouse T-Cell Workshop. Under these assay conditions, 16 of 16 NOD mice displayed proliferative responses to whole GAD65, 13 of 16 to GAD(524-543), 9 of 16 to GAD(217-236), 7 of 16 to insulin(9-23), and 5 of 16 to HSP277. Thus, spontaneous proliferative T-cell responses can be consistently detected to some beta-cell autoantigens and peptides thereof. Overall, the results suggest that more sensitive assays (e.g., ELISPOT, ELISA analysis of cytokines in supernatants, or tetramer staining) may be preferred for the detection of autoreactive T-cells.

Animals↗

[The quality of integration and care in workshops for the handicapped--The path to TQM in the workshops of the Gemeinschaftswerks fu"r Be- hinderte GmbH, Landstuhl].

Workshops for the Handicapped are exposed, as are other providers of social services, to powerful changes in social and economic conditions. Legal obligations (e.g. under the Federal Social Assistance Act BSHG), increasing customer demands and stronger competition make the ability to change a prerequisite, so that the quality and cost of providing services to the handicapped become a central theme. These changes in conditions have led the Gemeinschftswerk für Behinderte GmbH, Landstuhl, to intensively consider the themes Quality and Total Quality Management (TQM). The efforts to introduce TQM were concentrated initially on the DIN EN ISO 9000 certification of the production processes in the workshops. Currently, the themes of Integration and Care were examined. After the central processes had been identified the performance descriptions of system, structure, process, result, and steering committee were extended by process diagrams and responsibility matrixes. This paper describes the path that workshops of the Gemeinschaftswerk für Behinderte GmbH have taken towards TQM, with emphasis on presentation of the themes regarding quality of Integration and Care.

Delivery of Health Care, Integrated↗

[Patients with severe multiple handicaps in workshops for the handicapped--a project of the Berlin Workshops for the Handicapped GmbH (BWB) 4 December 1989 to 31 May 1990].

A project had been carried out at Berliner Werkstätten für Behinderte GmbH (BWB) aimed at integrating cerebral palsied persons with very severe, multiple disability in a workshop for the disabled. Client workshop readiness had been determined on the criteria of social capacity, extraordinary care need at the workplace, and economic viability of work. The project, planned to last 6 months, had to be discontinued because the 9 disabled persons included in this programme refused to cooperate after a short period of time.

Activities of Daily Living↗

Report on the 1997 International Society of Blood Transfusion Workshop for Genotyping of Platelet Alloantigens. Platelet and Granulocyte Workshop ISBT.

The results from the 1997 International human platelet antigen- (HPA-) genotyping workshop are reported. Seventeen institutions participated to genotype for HPA polymorphisms of seven DNA samples. Most investigators used the polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) and PCR-sequence specific priming (SSP). A few participants applied both methods to confirm their results. All participants reported results for HPA-1, -2, -3 and -5 allotyping, while HPA-4 was determined in 12 and HPA-6 in nine laboratories, respectively. For HPA-1 to -6 there were 19 deviations from the consensus result. These deviations were not associated with any particular technique or allotype. Most frequently, however, deviation were reported for HPA-5 typing. These results were discussed at a meeting at the Joint Congress of the ISBT and the Deutsche Gesellschaft für Transfusionsmedizin und Immunhämatologie in Frankfurt, Germany, in September 1997. Based on the results and the comments, further workshops for HPA-allotyping are mandatory for quality assurance.

Antigens, Human Platelet↗

Randomised trials of STD treatment for HIV prevention: report of an international workshop. HIV/STD Trials Workshop Group.

Three community trials of the impact of STD treatment interventions on HIV incidence in rural populations have been completed or are in progress in Uganda and Tanzania. Investigators from these trials met for a joint technical workshop in Baltimore in May 1996. This report summarises the consensus of the workshop, with the aim of providing useful input to research on HIV intervention strategies. Issues discussed include: (i) the role of community randomised trials; (ii) strategies for STD management; (iii) epidemiological and statistical issues in the design and analysis of community randomised trials; (iv) diagnostic methods for STDs in population surveys; (v) treatment regimens for STDs in rural Africa; and (vi) ethical issues in community trials.

Community Health Services↗

Biological products regulated under Section 351 of the Public Health Service Act; implementation of biologics license; elimination of establishment license and product license; public workshop--FDA. Proposed rule; notice of workshop.

The Food and Drug Administration (FDA) is announcing a public workshop to present issues related to the agency's proposed rule entitled "Biological Products Regulated Under Section 351 of the Public Health Service Act; Implementation of Biologics License; Elimination of Establishment License and Product License" issued recently in the Federal Register. The purpose of the public workshop is to provide interested persons an opportunity to more clearly understand the proposed rule and its effect on industry and the public.

Biological Products↗

Capacity building for research: report on a UNION Strategic Planning Workshop. Report of a workshop held during the International Union Against Tuberculosis and Lung Disease World Conference on Lung Health, Montreal, Canada, 7 October 2002.

The International Union Against Tuberculosis and Lung Disease (UNION) has a decade of experience in conducting short courses on research methods for the promotion of lung health. In a parallel initiative, the UNION has collaborated with the American Thoracic Society (ATS) to develop courses on international respiratory epidemiology that have been expanded by the ATS throughout Latin America. The two initiatives have similar aims, and the courses offered by the two organisations have intentionally been similar in terms of focus and complementary in regions targeted. The target regions of the UNION, drawing on several decades of experience in offering short courses on tuberculosis, have been Africa, the Black Sea Rim, the Middle East and Asia, while the target regions of the ATS have been Central and South America. This workshop aimed first to provide an opportunity for those who have taught on the courses and those who have participated to share their experiences and learn from them, and second to provide a basis on which to develop a strategic plan for future courses. This report summarises the contributions of the workshop participants and the conclusions reached.

Education, Medical, Continuing↗

[Workshops: the workshop as a didactic form for the presentation of clinical practice in public health nursing].

The use of the workshop as a didactical method in the presentation of clinical practica to community health nursing students at the distance teaching University of South Africa is described. The large number of students enrolled for this course at UNISA presented logistical problems to the services which offered opportunities for clinical practica to students. An outline of the course, as well as the objectives for the workshop, the contents, techniques applied and the evaluation is given.

Education, Nursing, Baccalaureate↗

National Institutes of Health workshop summary. Summary and recommendations of a workshop on the investigative use of fiberoptic bronchoscopy and bronchoalveolar lavage in individuals with asthma.

The participants in the workshop reached unanimous consensus that, as an investigative tool, BAL has enormous potential for extending knowledge of the immunopathogenesis of asthma. When BAL is used according to these guidelines, maximum knowledge may be gained with minimal risks to study subjects. However, we wish to emphasize that the extent to which the safety of the procedure applies to those subjects with asthma and more symptoms and an FEV1 of less than 60% of predicted value remains to be established by carefully controlled clinical investigations.

Asthma↗

Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens: results from the Second International Swine CD Workshop.

After initial evaluation of the 176 new and 19 control monoclonal antibodies (mAb) submitted to the Second International Swine CD Workshop, 57 were assigned to the T-cell/activation marker subgroup. These 57 mAb were further analyzed using flow cytometry on whole blood lymphocytes, splenocytes, Peyer's patch lymphocytes, in vitro cell lines, broncho-alveolar lavage cells, Con A and PHA blasts, fetal cell populations, and by 2-color flow cytometry against mAb to porcine CD2, CD4, and CD8. Finally, the molecular weights of the target antigens were characterized when possible. As a result of these analyses, 23 mAb were distributed into 7 CD clusters. Newly confirmed mAb assignments included: two CD2; one CD4; two CD5; one wCD6; and one wCD25. Three new mAb were found that reacted with wCD8, one of which defined a new epitope, wCD8c. For the first time, mAb against porcine CD3 were identified, including 6 mAb that reacted with three different epitopes. Several new mAb reacted with antigens whose expression varied depending on the activation state of the test cell. These will require further characterization in order to assign a CD number.

Animals↗

Workshop on childhood obesity: summary of the discussion.

Childhood obesity is rapidly emerging as a global epidemic that will have profound public health consequences as overweight children become overweight adults. To address this problem, action is needed at national and international levels. However, well-documented evidence of the trends in and global prevalence of obesity in children and adolescents is required to develop sound public health policies. There is no internationally acceptable index to assess childhood obesity nor is there an established cutoff point to define overweight in children. The purpose of the workshop was to establish a reasonable index with which to assess adiposity (overweight) in children and adolescents worldwide. We present here a summary of the discussion on the establishment of an index. The participants concluded that although body mass index (BMI; in kg/m2) is not a perfect measure in children because it covaries with height, it has been validated against measurements of body density. Because a consistent and pragmatic definition for overweight in children and adolescents is required, BMI may therefore be appropriate. However, other alternatives may be considered in the future. The group suggested a scheme for cutoff points for children and adolescents based on internationally accepted BMI cutoff points for adult morbidity of 25 and 30 to identify grade 1 and grade 2 overweight, respectively. Use of these cutoff points would provide a new approach to identifying childhood obesity and make the definition for children and adolescents consistent with that for adults.

Journal Article↗

ECNIS-sponsored workshop on biomarkers of exposure and cancer risk: DNA damage and DNA adduct detection and 6th GUM-32P-postlabelling workshop, German Cancer Research Center, Heidelberg, Germany, 29-30 September 2006.

Of all the chemicals classified as carcinogenic to humans by the International Agency for Research on Cancer (IARC), 90% exert their biological effects through binding of their metabolically activated intermediates to DNA forming covalent DNA adducts. As a consequence DNA adducts are generally considered to be causative and directly related to tumour formation. DNA adduct analyses reflect tissue-specific rates of adduct formation and removal, which depend on carcinogen uptake, metabolic activation, DNA repair, adduct instability and tissue turnover and are thus useful markers of carcinogen exposure. The measurement of carcinogen-DNA adduct levels is central to the understanding of chemical carcinogenesis both in animals and humans to determine molecular mechanisms and exposure. Sensitive methods for DNA adduct analysis used to date are based on (32)P-post-labelling, immunoassay, mass spectrometry and laser-induced fluorescence. The aim of this workshop held over 2 days (29-30 September 2006) at the German Cancer Research Center (DKFZ) in Heidelberg, Germany, was to discuss methodological improvements of DNA adduct detection with emphasis on the (32)P-post-labelling procedure as well as new findings achieved by applying the methods to studies on understanding human cancer mechanisms and to elucidate the relationship between adduct formation and human cancer risk.

Animals↗

A National Institutes of Health Workshop Report. Cellular and molecular mechanisms for suppression and reversion of tumorigenicity. A Chemical Pathology Study Section workshop.

A workshop organized by the Chemical Pathology Study Section was convened to discuss recent findings on the suppression and/or reversal of neoplastic transformation. The existence of specific genes involved in suppressing tumorigenicity has been clearly demonstrated by molecular and cytogenetic analyses of tumor and normal cells from individuals predisposed to develop specific malignancies and by studies of hybrids between normal and neoplastic cells. The malignancy of tumor cells can be suppressed in hybrids with normal cells despite the continued expression of activated oncogenes. Reversal of the malignant state can also be affected by placing tumor cells in certain embryonic environments, such as the mouse blastocyst, or in response to differentiation inducing factors. The numerous examples of suppression and reversal of malignancy indicate that this is an important area for future study which may lead to new methods to control cancer.

Animals↗

Introduction: workshop summary of the CD15 monoclonal antibody panel from the Fifth International Workshop on Leukocyte Antigens.

CD15 is expressed on a wide variety of tumor cells including myeloid leukemia, breast, colorectal, lung cancer cells. It is probable that the core proteins and lipids differ on the different cell types since there is marked variability in the binding of the mAb within and among the different cell types. Previously, there had only been a single mAb of the IgG class directed to CD15. The present study demonstrates the existence of one more IgG mAb (an IgG3). Unfortunately, a third mAb submitted as an IgG1, MA14, did not appear to be an IgG in our studies and a fourth mAb, MA9, submitted as an IgG1 did not have significant binding activity and also could not be confirmed as anti-CD15. An interesting finding was that all of the mAb could mediate complement-dependent cytotoxicity with rabbit serum. However, the two IgG3 mAb, MA63 (MCS-1) and MA88 (7C3), could not mediate lysis with human complement, while all of the IgM mAb could do so. It is not clear why this difference exists. CD15 is known to be expressed on mature myeloid cells in the hematopoietic lineage. The expression of CD15 on progenitor cells has been mapped by the indirect method of C'-dependent lysis and measurement of colony-forming cells. These studies have estimated that about 50% of normal CFU-GM express CD15. In the present workshop, we examined the expression of CD15 on a quantifiable cell population, the CD34 positive population.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗