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At least 37 records · Page 2Linked to original sources
PubMed1976

Intravenous infusion bottle plugs as a source of microbial contamination.

A technique was described for the artificial contamination of the upper surface of intravenous infusion container plugs with viable micro-organisms. This technique was employed to assess the adequacy of decontamination of two designs of plug by the use of antiseptic swabbing. Results indicated that the MRC plug surface was more difficult to sterilize than that of the Travenol plug. The possibility of contamination of the infusion fluid during insertion of the giving-set needle was examined. Results indicated that there was a risk of contamination through the administration-set port of the MRC plug if swabbing was inadequate.

Bacillus megaterium↗
PubMed1977

Rapid detection of microbial contamination in frozen vegetables by automated impedance measurements.

Automated impedance measurements can be used to rapidly assess whether a sample of frozen vegetables contains greater or less than 10(5) organisms per g. Microorganisms growing pureed food samples cause a change in the impedance of the medium when the organisms reach a threshold concentration of between 10(6) and 10(7) organisms per ml. Estimates of the concentration of microorganisms initially present in the food sample can be made by recording the time required for the organisms in the sample to replicate to threshold levels. In this study, the detection times for 357 samples of frozen vegetables were compared with standard plate counts for each sample. The agreement between the two methods in distinguishing samples containing more than 10(5) organisms per g was 92.6% for 257 assorted frozen vegetables and somewhat higher (93 to 96%) when separate cutoff times were used for each type of vegetable. The time required for analysis was about 5 h, compared to the 48 to 72 h required for standard plate counts.

Bacteria↗
PubMed1979

Wipe-rinse technique for quantitating microbial contamination on large surfaces.

The evaluation of an improved wipe-rinse technique for the bioassay of large areas was undertaken due to inherent inadequacies in the cotton swab-rinse technique to which assay of spacecraft is currently restricted. Four types of contamination control cloths were initially tested. A polyester-bonded cloth (PBC) was selected for further evaluation because of its superior efficiency and handling characteristics. Results from comparative tests with PBC and cotton swabs on simulated spacecraft surfaces indicated a significantly higher recovery efficiency for the PBC than for the cotton (90.4 versus 75.2%). Of the sampling areas sites studied, PBC was found to be most effective on surface areas not exceeding 0.74 m2 (8.0 feet 2).

Aircraft↗
PubMed1975

[Microbial contamination of air by activated sludge units (author's transl)].

Measurements of the germ emissions in various activated sludge units with different ventilations systems have shown that the lowest density occurs with small bubble ventilation; with medium bubble and brush ventilation rather high values were measured; the spray devices used for foam elimination had a particularly unfavorable influence. In great germ densities above the pools (50-100 000 germs/m3) - depending on the weather - between 500 and 1500 germs/m3 were measured at a distance of 50 and 100 meters. At distances of 200 and 400 m the number of germs was about the same as normally measured in the outside air (100-500 germs/m3). In a closed pool altogether considerably higher values were measured: In the immediate neighbourhood of the activated sludge unit they amounted to abt. 10 000-25 000 germs/m3; in the hall 3000-4000 germs/m3 were measured, whereby the part of coliform bacteria amounted to 1-2% and that of enterococci to 2-4%. It has to be taken into account that besides the measured intestinal flora further pathogenous agents are present in the aerosols. In closed units, therefore, a greater risk of aerogenous infections exists, whereas in open-air pools this danger can be judged as very small.

Aerosols↗