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PubMedDate not supplied

[The enzyme-linked immunosorbent assay (ELISA) in the diagnosis of amoebiasis (author's transl)].

An enzyme-linked immunosorbent assay (ELISA) technique as an aid to the detection of antibodies to Entamoeba histolytica has been evaluated in four groups of 39 individuals. Results were positive in 15 patients with amoebic dysentery harboring trophozoites of Entamoeba histolytica, in their stools; negative in 6 patients with enteric symptoms whose faeces contained neither trophozoites nor cysts of Entamoeba histolytica, in 8 patients with other parasitic diseases in whom clinical examination was not indicative of infection with Entamoeba histolytica, and in 10 sera from blood-donors. The ELISA has been shown to be a sensitive method of detecting antibodies to entamoeba histolytica.

Amebiasis↗
PubMed1979

ELISA for the serology of FIP virus.

An enzyme linked immunosorbent assay (ELISA) for feline infectious peritonitis (FIP) virus serology is described. The assay is analogous to a previously developed indirect heterologous immunofluorescence test (IFT) in which transmissible gastroenteritis (TGE) viral antigen was used. Comparative testing of selected feline sera in both assays resulted in corresponding titers, which justifies the conclusion that the ELISA is a reliable test for the serology of FIP virus.

Animals↗
PubMed1977

Serologic diagnosis of extraintestinal amebiasis: a comparison of stick-ELISA and other immunological tests.

The sera of 37 patients with amebic liver abscess and of 20 healthy donors were tested in Stick-ELISA (enzyme-linked immunosorbent assay), and the results compared to those obtained with complement fixation, indirect hemagglutination, counterelectrophoresis, and latex agglutination. Stick-ELISA results correlated positively with those of complement fixation and indirect hemagglutination at the one percent level.

Animals↗
PubMed1978

[Determination of antibodies against influenza viruses, using the ELISA method].

A microplate method of enzyme-linked immunosorbent assay (ELISA) was adapted for the demonstration of antibodies to influenza A and B in 25 paired acute and convalescent sera taken from patients with influenza A infection, in 11 single convalescent sera collected from patients with acute respiratory infection and serological evidence of influenza B infection, in 14 paired sera collected from volunteers who were vaccinated with an aqueous inactivated influenza A and B vaccine, and in 15 serum samples from normal subjects. Comparison showed that ELISA was more sensitive than the hemagglutination-inhibition test. The enzyme-immunoassay gave equally reliable and reproducible results and requires very small amounts of antigen and sera. This simple test may be suitable for use in large seroepidemiological surveys.

Antibodies, Viral↗
PubMedDate not supplied

The enzyme-linked immunosorbent assay (ELISA) (theory, technique and applications).

Enzyme-linked immunosorbent assays (ELISA) are useful for measuring levels on antigens and antibodies. Both competitive and sandwich methods can be used. These assays are analogous to radioimmunoassays in format and have similar sensitivity. However, results can sometimes by read visually and even for objective readings only simple equipment is needed. ELISA has been used to measure hormones and other protein antigens and has also been used to assay antibody levels.

Antibodies↗
PubMedDate not supplied

[Comparative study of immunoenzyme technics, ELISA and on formed antigens, in the immunologic diagnosis of African trypanosomiasis].

The enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of human african trypanosomiasis is described, comparatively with the immunoperoxidase technique using blood streamforms of Trypanosoma brucei as antigen. Practical aspects for measuring the amount of antibody in serum samples from infected rabbits and from patients with african sleeping sickness are discussed. ELISA does offer important advantages over the other method used namely: -- quantitative estimates are possible, -- it bends itself readily to large scale series, owing the fact that it can be fully automatic. These qualities will be turned to full account when the different classes of antibodies present in trypanosomiasis suffers can be examined by means of specific antigenic fractions.

Adolescent↗
PubMed1979

Detection of the K99 antigen of Escherichia coli in calf faeces by enzyme-linked immunosorbent assay (ELISA).

An enzyme-linked immunosorbent assay (ELISA) is presented for the detection of the K99 antigen of Escherichia coli in calf faeces. False-positive reactions were not observed with K99-negative strains and with several viral antigens. Only bovine coronavirus caused slight positive reactions which could be eliminated by a blocking test. As compared with the conventional procedure for the detection of the K99 antigen, ELISA seemed to be a least as sensitive and had the advantage that samples could be stored at --20 degrees C before testing. In addition many samples could be handled at the same time and the results became available quickly. By carrying out the assay as a blocking test, specific antibody against K99 in serum or colostrum could be detected and titrated.

Animals↗
PubMed1978

A direct enzyme-linked immunosorbent assay (ELISA) for detection of antibodies for rubella virus in human sera.

A direct enzyme-linked immunoassay (ELISA), based on the "sandwich" principle on an antigen-coated plastic disc, was used for the rapid detection of rubella antibody. Results were obtained the same day, and the prior adsorption of sera to remove non-specific inhibitors was not necessary. The ELISA was compared to the hemagglutination-inhibition (HAI) test on 500 serum samples. There was general agreement between the two methods; most discrepancies occurred with low-titered HAI positive sera. There was excellent correlation between the tests with serum samples negative for rubella antibodies and those samples with HAI titers greater than or equal to 1:40.

Animals↗
PubMed1979

Ocular Toxocara in siblings of two families. Diagnosis confirmed by ELISA test.

To my knowledge this study of two families with ocular Toxocara is the first in the literature to report involvement of more than one sibling. All four children had far-advanced disease with irreversible loss of macular vision in the affected eye. The clinical findings were confirmed by the ELISA test. With laboratory confirmation of the clinical findings, I expect to find not only more patients in the population at large with ocular Toxocara, but also expect to find numerous siblings in a particular family to be involved as well. Therefore, when ocular Toxocara is found in a child, every sibling in the family should be examined. Just as the young child with amblyopia does not complain of decreased vision, even so the young child with ocular Toxocara often will not complain of any visual problems.

Ascariasis↗
PubMed1979

A new principle for the detection of specific IgM antibodies applied in an ELISA for hepatitis A.

A new test principle for the detection of specific IgM-class antibodies was developed and applied in an Enzyme-Linked Immuno Sorbent Assay (ELISA) for the detection of hepatitis A IgM antibodies. A solid phase coated with anti-IgM was incubated successively with serum sample, specific antigen, and enzyme-labeled F (ab')2 fragments from IgG antibodies against the antigen and enzyme substrate. F(ab')2 fragments were used to avoid interference with rheumatoid factor. Specificity and sensitivity are very high. This test principle appears generally applicable in the diagnosis of infectious and parasitic diseases by testing only one serum sample.

Antibodies, Viral↗
PubMed1978

Rapid detection of IgG and IgM antibodies for cytomegalovirus by the enzyme linked immunosorbent assay (ELISA).

A simple solid phase enzyme immunoassay for the detection of immunoglobulin G and M to cytomegalovirus (CMV) is described. Using this test IgM antibodies to CMV were detected in 0.7 per cent of newborns and regularly after CMV infection in transplant patients, furthermore in these latter patients IgM production was prolonged for several months. For the determination of IgG the enzyme immunoassay was more sensitive than the complement fixation test (CF) and the antibody titres were 4 to 8 fold higher. Since the ELISA test is rapid, specific and unexpensive it can become an acceptable routine diagnostic procedure.

Adult↗
PubMed1979

Induction of specific anti-actin antibodies and their measurement by ELISA technique.

Using the ELISA technique we have been able to quantify antibodies directed against actin and to follow the kinetics of antibody production. Specific anti-actin antisera have been raised in rabbits by immunization with chemically modified white muscle rabbit actin. Two or three dinitrophenyl groups linked per actin molecule were sufficient to break natural tolerance, while linkage of three phosphorylcholine groups to actin was not.

Actins↗
PubMed1979

Diffusion in gel-enzyme linked immunosorbent assay (DIG-ELISA): a simple method for quantitation of class-specific antibodies.

A new method for quantifying class-specific antibodies is presented. The method has been named Diffusion-In-Gel-Enzyme-Linked-ImmunoSorbentAssay (DIG-ELISA), and is briefly as follows. Antiserum ia allowed to diffuse from wells in a gel layered over an antigen-coated plastic surface. The gel is then removed and the preparation is incubated with enzyme-conjugated anti-immunoglobulin. The enzyme is then visualised in situ by a colour reaction produced by pouring a substrate-containing gel over the plastic surface. Bovine serum albumin and rabbit-anti-BSA were used as a model system, and horseradish peroxidase or alkaline phosphatase as enzymes for visualization.

Alkaline Phosphatase↗
PubMed1979

Detection of antibodies to Sendai virus by enzyme-linked immunosorbent assay (ELISA).

An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Sendai virus, a paramyxovirus, is described. The assay was found to be about 20-fold more sensitive than the hemagglutination inhibition assay. Differentiation between virus specific IgG and IgM is possible. The test appears to be especially useful in the study of early events in antibody formation in vivo as well as in vitro.

Animals↗
PubMed1979

ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes.

A method is described which permits the adaption of ELISA techniques for measurement of antibody against bacterial polysaccharides. First, the polysaccharides antigen is covalently bound to poly-L-lysine, using cyanuric chloride as the coupling agent. The poly-L-lysine then adsorbs to the walls of plastic tubes, thus immobilizing the polysaccharide coupled to the poly-L-lysine. The method is simple, rapid, and utilizes small amounts of polysaccharide antigen.

Adsorption↗
PubMed1979

Application of the enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of Schistosoma mansoni infections in St. Lucia.

As part of our search for a serodiagnostic assay to replace the expensive and tedious stool examination in the diagnosis of Schistosoma mansoni infection, we have studied the sensitivity, specificity and quantitative features of an enzyme linked immunoassay (ELISA) using crude S. mansoni egg antigen preparation. Results of studies carried out in both London and St. Lucia indicate that the assay can give useful serodiagnostic information ranging from 82 to 99.5% sensitivity, depending on level of infection intensity and method of blood collection and 100% specificity in St. Lucian/St. Vincent populations. The St. Lucia study also showed that the assay could be operated in a qualitative form in an endemic area.

Adolescent↗