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Bacterial microleakage of barrier materials in obturated root canals.

The bacterial microleakage of four current restorative materials (glass ionomer cement, polycarboxylate cement, resin modified glass ionomer cement, and flowable composite resin) used as a base over obturated root canals were evaluated during a 5-month period. Sixty single-rooted mandibular premolars were obturated with cold lateral compaction of gutta-percha. The teeth were randomly divided into five groups of 10 teeth each and positive and negative control groups of five. The access openings were filled with one of the tested barrier materials in four groups. In group 5, no barrier material was placed. Samples were incorporated in a model system using Staphylococcus epidermidis as a microbial marker. Results were analyzed with Kaplan-Meier survival analysis (p = 0.05). The sealing ability of all tested materials was better when compared with group 5 (no barrier material) (p < 0.05). Within the limitations of this study, the glass ionomer cement leaked significantly less when compared with the flowable composite resin (p < 0.05).

Bicuspid↗

Apical placement of needle tip with an injection-thermoplasticized gutta-percha technique for root canal obturation.

It is recommended that when injection-thermoplasticized gutta-percha is used as the obturation technique, the root canal space should be prepared in such a way that the 23-gauge injection needle tip can be placed at a distance 3 to 5 mm short of the working length. In this study the apical seal and extent of gutta-percha were evaluated when the 23-gauge tip was placed at a distance 4 mm, 6 mm, and 8 mm short of the working length. Ninety-nine human single-rooted mandibular incisors and premolars divided into 3 equal groups were used. Their crowns were separated at the cementoenamel junction and their roots were stored in sodium hypochlorite for 1 week. The roots were then instrumented from size 20 at the working length to size 70:4 mm short of the working length in group A, 6 mm in group B, and 8 mm in group C. Following instrumentation, 3 randomly selected roots of each group were used as control. The remaining 30 roots of each group were covered with aluminium foil and embedded in acrylic blocks. The root canals were then dried, coated with sealer (Roth 811) and obturated by injection of thermoplasticized gutta-percha using the single-phase technique. Vertical condensation followed. The roots were immersed in dye for 3 days and then demineralized, dehydrated and cleared in xylene. Measurements of linear dye penetration for the apical seal and for underextension were done by 2 independent examiners under a stereomicroscope. The results of this study showed no statistically significant difference in leakage between the groups.(ABSTRACT TRUNCATED AT 250 WORDS)

Coloring Agents↗

Bacterial leakage versus dye leakage in obturated root canals.

AIM: The aim of this study was to compare in vitro bacterial and dye leakage tests, commonly used to determine the seal of root canal fillings. METHODOLOGY: Ninety-six single-rooted teeth had their crowns removed at the cemento-enamel junction and their roots instrumented to ISO size 60 within 1 mm of the apex. Three groups of 30 roots were obturated by lateral condensation using gutta-percha and one or other of the sealers. AH26, Ketac Endo, or Roth's 801 sealer. Three roots were used as negative controls and three roots as positive controls. The roots were then exposed at their coronal end first for 38 days to soy broth containing Staphylococcus epidermidis and thereafter for 48 h to basic fuchsin. Bacterial leakage was recorded when the challenging organism could be recovered from the apical end. Dye leakage was checked by microscopy of transverse sections of the apical tip at the end of the experiment. RESULTS: For the bacterial experiment, there was no significant difference amongst the three sealer groups. The dye experiment showed significantly greater leakage in the AH26 compared to the Ketac Endo group. No correlation between the results of the two tests could be seen. CONCLUSIONS: The results of this study suggest that the molecular size of the penetrating agent may not be the relevant parameter when attempting to determine an appropriate test for the sealability of root canal fillings.

Bismuth↗

[Results and consequences of clinical-radiological follow-up studies of root canal treated teeth. 2. Dependence of the results on age, degree of root canal obturation and devitalizing drugs. Conclusions for the dental practice].

The authors report of the follow-up examination of root-canal treatments performed at the Policlinic of Conservative Stomatology of the Karl Marx University of Leipzig in 1967, in which 499 teeth were assessed using severe criteria. The results obtained from the various diagnostic groups depended on the age and the degree of root-filling. Of the root-canal treatment techniques employed, devitalization by means of arsenic showed the highest failure rate. Conclusions relevant to stomatological practice are drawn from the results presented in the two parts of this study.

Age Factors↗

Coronal leakage of obturated root canals after long-term storage using a polymicrobial marker.

This in vitro study investigated the effect of long-term storage on the coronal leakage of a microbial marker on teeth root filled with lateral condensation of cold gutta-percha and one of two sealers. Sixty single-rooted teeth were prepared chemomechanically to a size 40 master apical file. The teeth were divided into two groups of 20 teeth each and obturated with gutta-percha using either Apexit or Tubliseal EWT sealer. The teeth were stored for 6 months in artificial saliva and tested for leakage using a marker consisting of S. sanguis and P. intermedia. The teeth were checked for bacterial leakage daily for 90 days. All positive control teeth leaked after 24 h, while the negative control teeth remained uncontaminated throughout the test period. Leakage reached the apex through the experimental teeth at the earliest at 17 days and at latest at 88 days. Fifty percent and 70% of the specimens of the Apexit and Tubliseal EWT groups respectively showed leakage at 90 days. There was no statistically significant difference (p > 0.05) between the two groups.

Calcium Hydroxide↗

Root surface temperature rises in vitro during root canal obturation with thermoplasticized gutta-percha on a carrier or by injection.

The aim of this in vitro study was to measure the temperature rise on the outer root surfaces of teeth during four different root canal obturation techniques. Sixty extracted human maxillary and mandibular premolars with a single canal were used. After root canal cleaning and shaping, the teeth were randomly divided into four groups of 15 teeth each and obturated with Thermafil obturators or Soft-Core obturators using Ultrafil or Trifecta low-temperature thermoplasticized gutta-percha techniques. Temperature changes on the external mesial root surfaces were measured using a thermal imaging camera. Lower temperature rises were found for Ultrafil and Trifecta techniques (2.14 degrees C and 2.03 degrees C, respectively) than for Thermafil and Soft-Core techniques (3.87 degrees C and 3.67 degrees C, respectively). These findings suggest that solid core gutta-percha combined with low-temperature injectable gutta-percha obturation techniques may impose less risk for thermal damage to the surrounding periradicular tissues.

Analysis of Variance↗

An in vitro evaluation of the apical sealing ability of a new resin-based root canal obturation system.

The purpose of this study was to assess the apical sealing ability of the new resin-based Epiphany-Resilon root canal filling system, and to compare this with the sealing abilities of different pairings of AH plus, gutta-percha, Epiphany, and Resilon. Seventy extracted human single-rooted teeth were used. All teeth were instrumented using a set of ProTaper rotary instruments. The canal spaces were filled with different combinations of core and sealer using lateral condensation, as follows: group 1, AH Plus + gutta-percha; group 2, AH Plus + Resilon; group 3, Epiphany + Resilon; group 4, Epiphany + gutta-percha. Apical leakage quantity was measured with the computerized fluid filtration meter. Statistical analysis indicated that Epiphany gutta-percha combination had the least amount of microleakage than all the other groups; AH Plus gutta-percha combination proved to have second least amount of microleakage among the groups. AH Plus-Resilon combination demonstrated the greatest amount of microleakage. There were a significant difference between Epiphany gutta-percha and AH Plus-Resilon combinations (p < 0.05). It was found that there were no significant differences between Epiphany-Resilon combination and all the other groups (p > 0.05).

Dental Bonding↗

Effect of a final alcohol rinse on sealer coverage of obturated root canals.

An endodontic sealer is required to provide an apical and coronal seal in root canals. No previously reported methods of sealer placement completely coated canal walls. The purpose of this study was to dehydrate canal walls with alcohol and determine if this permitted better sealer coverage. Forty single-rooted teeth were prepared and divided into 4 groups of 10 teeth according to method of sealer placement and final irrigant: (a) by lentulo spiral and (b) file NaOCl, (c) lentulo and alcohol, and (d) file and alcohol. After canal preparation, groups 1 and 2 were irrigated with 1 ml of NaOCl and dried with paper points. Groups 3 and 4 were irrigated with 1 ml of alcohol and dried. AH26 (0.04 ml) dyed with charcoal black was applied to the apical end of the file or lentulo and placed in the canal to coat the walls. Gutta-percha without additional sealer was laterally condensed. Teeth were cleared and evaluated for location and amount of sealer visible. Data were evaluated by Kruskal-Wallis and analysis of variance. The results showed (a) no significant differences among the four groups, (b) all areas had sealer present, (c) the coronal third had > 50% sealer coverage in nearly all teeth, and (d) the middle and apical thirds showed the most variability in sealer coverage.

Analysis of Variance↗

Effect of root-end resection and root-end filling on apical leakage in the presence of core-carrier root canal obturation.

AIM: To evaluate the apical seal of canals filled with a core-carrier obturator following root-end resection with and without a root-end filling. METHODOLOGY: Thirty single-rooted human teeth with single canals were used. Root canal treatment was performed and canals filled with a core-carrier obturation technique. The teeth were then randomly assigned to three groups (n = 10). In the first group root canal filling only was performed. After orthograde filling, the teeth of the second group were resected apically, perpendicular to the major axis of the root. In the third group after apical resection, a root-end cavity was prepared using ultrasonic diamond retrotips and the cavities filled with Super-EBA cement. During a period of 3 h and with a headspace pressure of 0.12 atm, methylene-blue solution was forced through a tube that was connected to the apical end of each tooth specimen. The coronal end of the tooth was connected to a capillary tube containing an air bubble. Leakage was evaluated by observing the distal displacement of the air bubble. The roots were then sectioned along their long axis. Using a stereomicroscope, linear dye infiltration at the dentine-cement interface was determined. Kruskal-Wallis and Mann-Whitney tests were used to compare the three groups. RESULTS: Linear dye infiltration was significantly greater in root canals filled with the core-carrier obturators and resected apically (0.9 +/- 0.9 mm) when compared with those that had root-end fillings (0.2 +/- 0.4 mm). Air bubble displacement was not observed. CONCLUSION: Root-end filling improves the sealing of roots with core-carrier obturation following root-end resection.

Adolescent↗