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Modified behavioral and olfactory bulb responses to maternal odors in preweanling rats.

Rat pups acquire an attraction for maternal odors, which can vary with maternal diet. In the two experiments reported here, maternal diet was modified and both pup behavioral responses and pup olfactory bulb neural responses [( 14C]2-DG uptake) to maternal odors were examined. In experiment 1, pups were reared from birth to postnatal day 19 with either a dam fed normal rat chow or a dam fed a sucrose-based diet which suppressed her normal maternal odor. In experiment 2, pups were raised from birth to postnatal day 19 with either a dam fed the sucrose-based diet adulterated with peppermint, or the non-scented sucrose-based diet. Pups selectively expressed both a behavioral attraction and an enhanced olfactory bulb neural response to odors that they experienced in the nest.

Action Potentials↗

Differential effects of prenatal protein malnutrition and prenatal cocaine on a test of homing behavior in rat pups.

The effects of prenatal cocaine exposure and protein malnutrition on orientation to home nest material was assessed in rat pups. Sprague-Dawley dams were fed a diet of low protein content (6% casein), and isocaloric diet of adequate protein content (25% casein, control), or a laboratory chow diet prior to mating and throughout pregnancy. Within each diet group, rats received either cocaine injections (30 mg/kg IP 2 times per week prior to mating and the 30 mg/kg SC daily from day 3 to 18 of pregnancy) or saline injections. All litters were fostered on the day of birth to control mothers fed an adequate diet. On postnatal days 7, 9, and 11, a single pup from each litter (n = 11-15 per treatment) was tested repeatedly in a clean test cage for the rapidity of approach, and level of attraction to their own home (nest) bedding compared with fresh bedding. Prenatal malnutrition and prenatal cocaine exposure each gave rise to independent effects on performance, based upon factor analysis. Prenatal malnutrition, but not prenatal cocaine increased the time taken for rat pups to approach their nest bedding, reduced the time spent on this bedding, decreased the number of entries into the sector containing the home bedding and reduced pup weight. Prenatal cocaine, but not prenatal malnutrition, produced a reduction in activity, but had no effect on pup weight. The lower activity level was most pronounced on postnatal day 7. Surprisingly, interactive effects of prenatal cocaine and prenatal malnutrition were not observed on any behavior examined. Nevertheless, the co-existence of drug addiction and malnutrition in human populations raises the possibility that some of the effects generally attributed to drug exposure may, in fact, be due to malnutrition.

Animals↗

Social settings and situations of underage drinking.

OBJECTIVE: Social settings and situations of underage drinking were described for students from 15 communities in Minnesota and Wisconsin. Reports of their last drinking event, including setting, number of persons drinking with them, number of those persons under age 21, and whom they were with, were examined. The role of these variables in the prediction of having five or more drinks on one occasion was assessed. METHOD: Ninth graders (n = 2,269) and 12th graders (n = 2,377) who reported using alcohol in the last 30 days were included in the sample from a nested cross-sectional survey design. Bivariate analyses were performed between the situational variables and gender, number of older siblings and drinking behavior. Chi-square statistics were divided by an estimate of the design effect and multivariate analyses used mixed-model regression to correct for the nesting of individuals within communities. RESULTS: Situations and settings of drinking differed according to age and drinking behavior. Twelfth graders were less likely to drink with parents or other adults than 9th graders and more likely to drink in someone else's home, and in large groups where nearly everyone was underage. Persons reporting having five or more drinks on one occasion in the last 2 weeks were more likely to report drinking with peers, in large groups of underage persons and away from home. CONCLUSIONS: Interventions to reduce use of alcohol by youth must focus on the context in which the drinking is taking place in addition to other factors. Policy or educational interventions that seek to alter the situations and settings of underage drinking may be effective in reducing consumption of alcohol in adolescents.

Adolescent↗

[Light and activity rhythm of Phaner furcifer (nocturnal malagasy Prosimian) in its natural environment (author's transl)].

The behavior of a nocturnal malagasy Prosimian (Phaner furcifer) in its natural environment has been studied in relation to its visual abilities and to the light cycle of the biotope. By photometric measurements (autonomous photomultiplier used with interferential filters) we attempted to connect the activity rhythms of the animals with the light level in the actual biotope (quantitative and qualitative approach). 10 Emergence from the nest occurs when the level of light becomes sufficiently low (about 1 lux). Return to the nest is observed as soon as the light level increases. The times of these behavioral activities therefore vary with the season. 20 Histological examination of the retina reveals only the presence of rods. The absolute sensitivity threshold (scotopic) measured by a technique of operant conditioning is comparable to that of man. 30 The level of light observed in nature is well above the visual threshold. The precision with which emergence from the nest occurs in relation to the ambiant light is surprising considering the absence of cones in the retina.

Activity Cycles↗

Multidimensional latent-construct analysis of children's social information processing patterns: correlations with aggressive behavior problems.

Social information processing (SIP) patterns were conceptualized in orthogonal domains of process and context and measured through responses to hypothetical vignettes in a stratified sample of 387 children (50% boys; 49% minority) from 4 geographical sites followed from kindergarten through 3rd grade. Multidimensional, latent-construct, confirmatory factor analyses supported the within-construct internal consistency, cross-construct discrimination, and multidimensionality of SIP patterns. Contrasts among nested structural equation models indicated that SIP constructs significantly predicted children's aggressive behavior problems as measured by later teacher reports. The findings support the multidimensional construct validity of children's social cognitive patterns and the relevance of SIP patterns in children's aggressive behavior problems.

Aggression↗

The role of the accessory and main olfactory systems in maternal behavior of the primiparous rat.

The objective of this study was to analyze the respective roles of the main and accessory olfactory systems in the development of maternal behavior in the primiparous Wistar rat. Females underwent one of the following treatments: vomeronasal nerve section (VN), irrigation of nasal cavities with 5% ZnSO4 solution (ZN), surgical control, saline irrigation control, and normal control. Surgical or first irrigation were done before mating occurred. Irrigations were done every 7 days thereafter. The dams and their litters were observed from the day the litter was born (Day 1) through Day 16. Pup weights and temperatures were recorded daily. Home cages were checked daily for changes in nest location and number of times pups were found out of the nest. Retrieving tests were conducted on Days 4, 7, 10, and 13. The following behavioral items were observed: number of pup retrievals , number of times mother nosed or licked pups, percentage of litter returned to nest by end of test, dam self-grooming, dam climbing or rising, and dam digging or burrowing in shavings. The VN dams and their litters were not significantly different from the control dams and their litters on any of the measures taken. The ZN dams and their litters were not significantly different from their controls on all measures except for pup body temperature which was slightly lower from Day 13 through day 16 and pup body weight which was slightly lower from Day 12 through Day 16. Since these differences are very small, they do not seem to indicate a serious deficiency in maternal behavior. The results indicate that adequate maternal behavior develops with either of the two systems intact.

Animals↗

Why do physicians think parents expect antibiotics? What parents report vs what physicians believe.

OBJECTIVE: To examine the relation between parent expectations for antibiotics, parent communication behaviors, and physicians' perceptions of parent expectations for antibiotics. STUDY DESIGN: A nested cross-sectional study with parallel measures of parents presenting children for acute respiratory infections (previsit) and physicians (postvisit) and audiotaping of the encounters. POPULATION: Ten physicians in 2 private pediatric practices (1 community-based and 1 university-based) and a consecutive sample of 306 eligible parents (response rate, 86%) who were attending sick visits for their children between October 1996 and March 1997. OUTCOMES MEASURED: Communication behaviors used by parents expecting antibiotics and physicians' perceptions of parents' expectations. RESULTS: Parents' use of "candidate diagnoses" during problem presentation increased the likelihood that physicians would perceive parents as expecting antibiotics (from 29% to 47%; P=.04), as did parents' use of "resistance to the diagnosis" (an increase from 7% to 20%). In the multivariate model, parents' use of candidate diagnoses increased the odds that a doctor would perceive a parental expectation for antibiotics by more than 5 times (odds ratio, 5.23; 95% confidence interval, 3.74-7.31; Plt.001), and parents' use of resistance to a viral diagnosis increased these odds by nearly 3 times (odds ratio, 2.73; 95% confidence interval, 1.97-3.79; Plt.001). CONCLUSIONS: Parents perceived as expecting antibiotics may be seeking reassurance that their child is not seriously ill or that they were correct to obtain medical care. Physicians were significantly more likely to perceive parents as expecting antibiotics if they used certain communication behaviors. This study revealed an incongruity between parents' reported expectations, their communication behaviors, and physicians' perceptions of parents' expectations.

Anti-Bacterial Agents↗

The emergence of collective foraging in the arboreal Gnamptogenys menadensis (Hymenoptera: Formicidae).

Gnamptogenys menadensis is an arboreal nester that forages opportunistically almost exclusively on vegetation, sometimes recruiting others to participate in prey retrieval. The three-dimensional characteristics of vegetation suggest that functions describing recruitment decision thresholds or the pattern of recruitment in arboreal species may differ from those predicted by optimal foraging theory. To examine the effects of prey abundance and distance on the recruitment dynamics of G. menadensis, we baited nests with one termite, five termites or a number of termites between 20 and 40 either near to or far from the entrance and observed the ensuing behaviors. G. menadensis recruited others when encountering multiple termites regardless of the termite pile's distance from the nest, although a few individuals remained at the site and defended the resource. The pattern of arrivals at the site indicates that the majority and sometimes all arrivals were recruited from the branch trails. In combination, these results suggest that the architecture of the foraging habitat, which limits available return routes to the nest and thus increases encounter probabilities with potential recruits, shaped the process of information transfer and generated a collective pattern of foraging and prey retrieval.

Animals↗

Olfactory bulbectomy impairs the rat's radial-maze behavior.

Previous studies had shown that transection of the lateral olfactory tracts and lesions of the septohippocampal system were similar in the deficits they engendered in nest building and food piling or hoarding by hamsters. The present investigation asked whether olfactory lesions would also produce deficits in radial-maze behavior like those associated with septo-hippocampal lesions. Radical olfactory bulbectomy led to a marked deterioration in preoperatively trained behavior in an 8-arm radial maze. The behavioral changes resembled those resulting from septo-hippocampal lesions in three ways: (1) There were fewer choices of adjacent arms and more choices of more distant arms. (2) Choice accuracy as a function of successive choices within a trial declined more rapidly. (3) The probability of repeating choice sequences was abnormally high. Earlier studies had shown that the rat does not require olfactory cues to perform efficiently in radial mazes. It therefore seems likely that the deficits described here resulted from the disruption of interconnections between the olfactory and septo-hippocampal systems rather than from the loss of olfactory sensitivity.

Animals↗

Effects of preweaning exposure to novel maternal odors on maternal responsiveness and selectivity in adulthood.

We examined the effects of odor exposure in the nest on the offspring's subsequent adult responsivity to pups scented with the same odor or a different odor. It was hypothesized that animals receiving exposure to the odor in the nest would be more maternally responsive to pups scented with the (same) exposed odor than to unscented (water-scented) pups. In the first part of the study (Part A), Sprague-Dawley female rat pups (days 1-18 of age) either received exposure to an artificial lemon odor or to the natural (water) odor in the nest. In the second part of the study (Part B), rat pups were exposed daily to lemon or neutral odorants in an incubator at a distance from the mother and the nest. On day 21, animals received odor preference tests for lemon versus neutral pine bedding. On day 60, animals were mated and their pups were removed at 15-min intervals at the time of parturition. Animals were then tested for maternal behavior to foster pups on day 1 or 7 after parturition. Foster pups were scented either with the lemon odor or with water (unscented). Early exposure to lemon in the nest context enhanced animals' attraction to the lemon odor in juvenile tests. In adult maternal tests, exposure to odor on the mother and in the nest had two effects. It increased the latency for animals to express maternal behavior; however, once mothers expressed maternal behavior, they spent more time licking and crouching over pups scented with the same odorant to which they had been exposed earlier on their own mothers. Simple exposure to the lemon odorant out of context of the nest had no effect on adult maternal latencies or behavior.

Animals↗

Male-female interactions across the female estrous cycle: a comparison of two species of dwarf hamster (Phodopus campbelli and Phodopus sungorus).

Recent studies in our laboratory have suggested that monogamy may be the preferred mating system in the Djungarian hamster (Phodopus campbelli), whereas the available evidence for the closely related Siberian hamster (P. sungorus) does not show the same pattern. Here we examine the behavior of male-female dyads of both species interacting during 1-hr tests in large, familiar habitats containing defensible nest boxes, food, and water. Levels of aggression within pairs were low, compared with those seen during brief intrasexual encounters, whereas affiliative behaviors, such as sniffs, were high. P. campbelli scent marked more than twice as frequently as P. sungorus. Females of both species scent marked at a constant rate irrespective of their location in the habitat, whereas males scent marked at a higher rate in the female's home area. Two major features of the copulatory pattern differed between the two species: (a) The duration of the ejaculatory lock was five times longer in P. sungorus than in P. campbelli. (b) Both species had approximately the same number of mounts in each ejaculatory series, but the intromission/mount ratio was significantly higher in P. sungorus.

Agonistic Behavior↗

Prenatal protein malnutrition and home orientation in the rat.

Home orienting behavior was studied during the first 11 days of life in prenatally protein-malnourished and well-nourished rat pups, both groups having been reared by well-nourished mothers since birth. On all days of testing, mean weights were compromised in the prenatally malnourished pups relative to their well-nourished age controls (body weight deficit = 19-29%). Eye opening was also significantly delayed in the malnourished group. On the test of homing behavior, malnourished pups were significantly impaired in their ability to locate the nest on days 9 and 11. The effect was not related to activity levels of the pups, which were generally similar in the two nutritional groups. Thus, prenatal malnutrition disturbs the development of the homing response in the early postnatal period.

Animals↗

Stimulus control of behavioral inhibition in the preweanling rat.

Previous studies demonstrate that 14-day-old rats reduce their emission of ultrasonic vocalizations and freeze when exposed to an unfamiliar adult male rat. This study sought to identify the stimulus characteristics of conspecific males that potentiate the display of behavioral inhibition. In Experiment 1, day 14 rats were isolated from the nest and exposed to either an unfamiliar prepubescent male rat or an unfamiliar adult male rat. Pups exposed to the unfamiliar adult male rat exhibited significantly elevated levels of freezing and reduced their emission of ultrasounds. In Experiment 2, pups were exposed to either a familiar or an unfamiliar adult male rat. Although several pups exposed to the familiar adult male rat exhibited freezing, pups tested with the unfamiliar adult rat showed a reliably higher duration of freezing and made fewer vocalizations. Results suggest that neither the unfamiliar factor nor cues associated with male adulthood are sufficient to account for the occurrence of behavioral inhibition when presented separately. However, the combination of unfamiliarity and adult male stimuli are highly potent stimulus features that elicit behavioral inhibition in preweanling rats.

Animals↗

Juvenile hormone in adult eusocial Hymenoptera: gonadotropin and behavioral pacemaker.

Studies on the role of juvenile hormone (JH) in adult social Hymenoptera have focused on the regulation of two fundamental aspects of colony organization: reproductive division of labor between queens and workers and age-related division of labor among workers. JH acts as a gonadotropin in the primitively eusocial wasp and bumble bee species studied, and may also play this role in the advanced eusocial fire ants. However, there is no evidence that JH acts as a traditional gonadotropin in the advanced eusocial honey bee or in the few other ant species that have recently begun to be studied. The role of JH in age-related division of labor has been most thoroughly examined in honey bees. Results of these studies demonstrate that JH acts as a "behavioral pacemaker," influencing how fast a worker grows up and makes the transition from nest activities to foraging. Hypotheses concerning the evolutionary relationship between the two functions of JH in adult eusocial Hymenoptera are discussed.

Age Factors↗

From molecular to molar: a paradigm shift in behavior analysis.

A paradigm clash is occurring within behavior analysis. In the older paradigm, the molecular view, behavior consists of momentary or discrete responses that constitute instances of classes. Variation in response rate reflects variation in the strength or probability of the response class. The newer paradigm, the molar view, sees behavior as composed of activities that take up varying amounts of time. Whereas the molecular view takes response rate and choice to be "derived" measures and hence abstractions, the molar view takes response rate and choice to be concrete temporally extended behavioral allocations and regards momentary "responses" as abstractions. Research findings that point to variation in tempo, asymmetry in concurrent performance, and paradoxical resistance to change are readily interpretable when seen in the light of reinforcement and stimulus control of extended behavioral allocations or activities. Seen in the light of the ontological distinction between classes and individuals, extended behavioral allocations, like species in evolutionary taxonomy, constitute individuals, entities that change without changing their identity. Seeing allocations as individuals implies that less extended activities constitute parts of larger wholes rather than instances of classes. Both laboratory research and everyday behavior are explained plausibly in the light of concrete extended activities and their nesting. The molecular- view, because it requires discrete responses and contiguous events, relies on hypothetical stimuli and consequences to account for the same phenomena. One may prefer the molar view on grounds of elegance, integrative power, and plausibility.

Animals↗

Adenomatous tumors of the middle ear and mastoid.

Three cases of middle ear and mastoid neoplasms are reported as "adenomatous tumors" since in their search of the literature the authors did not find any previously described lesions with a similar histologic appearance and benign biologic behavior. Microscopically, all three tumors are similarly composed of solid cords and nests of closely-packed small cells having an epithelial appearance. Two distinct cell types are present: cuboidal cells, arranged in a rudimentary gland-like pattern, and angular cells forming irregular nests with no distinct pattern. All three tumors developed in patients in their 20's, over a period of months with minimal symptoms; yet in all of the lesions the tympanic membrane was intact at the time of initial examination. None of the neoplasms was diagnosed preoperatively, and, once removed, all three tumors were pathologic enigmas and therapeutic problems in view of the initial and subsequent consultant pathologic opinions; nevertheless, total local excision with preservation of the tympanic membrane would appear to be safe treatment in these cases. The term "adenomatous tumor" is applied to these three neoplasms because: 1. a true glandular epithelial origin warranting the term adenoma or adenocarcinoma cannot be proven; and 2. the biologic behavior and prognosis is not necessarily reflected by the histologic appearance. A more specific term reflecting the origin and behavior of these tumors does not appear possible without the study of further cases.

Adenocarcinoma↗

[Circadian and seasonal changes in the temperature-regulating behavior of the American ground squirrel Citellus parryi].

Under uninterrupted (during 10 days) temperature registration from 4 nest boxes ("thermogradient" plant) clear-cut changes were determined: in preferable temperature (PT, degrees C, in 24 hrs at an average)--21.0 +/- 0.5 (spring), 22.7 +/- 0.3 (summer), 18.8 +/- 0.7 (autumn), 11.5 +/- 0.5 (period before hibernation-PBH), 7.5 +/- 7.9 (hibernation season); in locomotor activity duration (LA, per cent from 24 hrs) which has decreased from 29.6% in spring to 20.3% at PBH (p < 0.001), and approximately to 2-3% during hibernation season; and in body mass which has varied from 350 g in spring to 750 at PBH. An increase in LA circadian rhythm amplitude was revealed in autumn, and the appearance and a rapid increase in PT circadian rhythm amplitude were observed at the beginning of PBH. A stage-to-stage "upswinging" of activity/rest circadian rhythms (stage I) and of thermoregulatory behavior (stage II) are supposed to occur at the end of euthermy season, which promotes synchronizing of the physiological processes and extending of the thermoneutra zone before the entrance into torpor and hibernation.

Animals↗

Sex differences in cooperative silk-spinning by weaver ant larvae.

Final-instar larvae of weaver ants (Oecophylla longinoda) use their silk for nest construction rather than for cocoon spinning; this commitment represents a shift from entirely selfish to entirely cooperative behavior that occurred after the emergence of the phylogenetically advanced subfamily Formicinae. Male larvae were found to have smaller silk glands and to contribute substantially less silk to nest construction. The hypothesis of kin selection appears to be the most plausible and parsimonious explanation of this sex difference, but other, individual-level modes of selection have not been eliminated.

Journal Article↗