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Results for “Epithelial Attachment”

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At least 19 recordsLinked to original sources
PubMedDate not supplied

A technique for the removal of junctional epithelium from the epithelial attachment.

A technique utilizing sodium deoxycholate to remove junctional epithelial cells from the epithelial attachment zone is described. Light and electron micrographs show a similarity in size and appearance between the extracellular attachment substance in controls and the extracellular substance which remains on cementum previously in contact with junctional epithelium.

Animals↗
PubMed1992

Ultracytochemical localization of basal lamina anionic sites in the rat epithelial attachment apparatus.

The basal lamina anionic sites of the epithelial attachment apparatus (EAA) were investigated at the electron microscopic level in adult rat periodontium. After 1M NaCl junctional epithelium detachment, an irregular and fluffy basal lamina-like structure appeared to cover the cementum surface. This structure reacted positively with polyethyleneimine (PEI), a strongly cationized ultrastructural tracer, appearing to be composed of highly electron-dense microaggregates. Depending on section plane, double-tracked structures of undefined length were found within PEI precipitates and closely related to cementum collagen fibrils. After nitrous acid de-N-sulphation, 8 nm wide sets of two parallel lines were clearly identified. "Double tracks", i.e., sets of paired lines with peripherical PEI electron-dense material, were found to self-assemble to form dimers, clusters or more complex organizational patterns. From sensitivity towards nitrous acid oxidation and positive control observations, it was concluded that basal lamina anionic sites in the EAA, represented by PEI microaggregates, contain heparan sulfate proteoglycans (HSPGs). Furthermore, high resolution ultrastructural images demonstrated that HSPGs adopt a morphological appearance of "double tracks" in the tissue. On the other hand, the present findings suggest that HSPGs clusters, never found in the mucosal basement membrane used as positive control, may be related to a functional specificity of the tissue at the dento-gingival junction.

Animals↗
PubMed1981

A method of obtaining an epithelial attachment on an endosseous implant.

An attempt was made to obtain a soft-tissue seal around the neck of an endosseous implant using a section of root from another tooth in the oral cavity. Six implants were placed bilaterally in the mandibular first molar regions of three monkeys. Four of the six implants were lost during the experimental period. In at least three of the four situations, it was apparent that the animals had removed the implants despite chair restraint. On the basis of clinical evaluation of all implants and histologic examination of the two remaining implants, it was concluded that an epithelial attachment was not present. The absence of an attachment may have been caused by chronic inflammation around the implant wafer. While an epithelial attachment could not be demonstrated in this experiment, it is felt that the rationale for this research project remains valid. Several potential improvements in methodology are suggested.

Animals↗
PubMed1990

[Development and significance of the cuticula dentis for the epithelial attachment].

The purpose of this study was to identify those conditions governing presence and function of the secondary cuticle (cuticula dentis) by means of a clinical evaluation (Periodontal Status, Plaque Index, Decayed-Missing-Filled-Index) as well as radiological, bacteriological (plaque swabs) and histological studies. The secondary cuticle could be demonstrated mainly in individuals with mild bone resorption and minimal loss of periodontal attachment (age 20-50 years). In most cases there was an intimate attachment of the junctional epithelium to the enamel or cementum surface. However, in most elderly subjects (older than 50 years) the secondary cuticle was found only in fragments or was not detectable at all. In these cases the junctional epithelium had proliferated apically (towards the apex dentis) and was only punctiformly attached to the tooth surface. Our histological findings suggested that the secondary cuticle is a secretory product of the junctional epithelium. Therefore, we assume it to be the essential structure in maintaining epithelial attachment.

Adolescent↗
PubMed1982

Progressive replacement of epithelial attachment by a connective tissue junction after experimental periodontal surgery in rats.

The purpose of this study was to test the hypothesis that the long junctional epithelium formed during the early stages of periodontal wound healing can become replaced, at least in part, by a connective tissue attachment. A surgical wound was created on the mesial surface of the left maxillary first molar of 4-month-old rats and the mesial root surface curetted free of soft tissue and cementum. The contralateral molar served as an unoperated control. Groups of five to eight animals were killed at intervals of 10 days, 3 and 6 weeks, and 3, 6 and 12 months after surgery. The histometric data demonstrated a progressive apical recession of the entire gingival unit on the unoperated control side during the experimental period. On the operated side, the junctional epithelium became reestablished by migration of epithelium from the wound edge along the cut gingival surface facing the tooth, until contact was established near the apical border of the instrumented root surface. Coronal migration of the epithelium from that level accounted for most of the lengthening of the junctional epithelium observed in 3 weeks. During the balance of the experimental period the junctional epithelium did not change in length significantly. However, the entire epithelial attachment was displaced coronally, primarily at the expense of sulcus depth which decreased with time, and by replacement of the apical portion of the junctional epithelium by a connective tissue junction of increasing dimension. These findings challenge the widely held belief that, once established, the dentoepithelial junction cannot be replaced by a connective tissue attachment.

Animals↗
PubMed1994

The epithelial attachment and the dental junctional epithelium: ultrastructural features in porcine molars.

The region of epithelial apposition with a tooth surface is the site of an unusual stratified integument, the junctional epithelium, which combines tight attachment to the tooth, cell turnover, tissue permeability, and epithelial versatility into the first line of defense against periodontal destruction by oral pathogens. To better understand the structure and function of the junctional epithelium we have reviewed its developmental and cell biology, and undertaken a multidisciplinary analysis of its composition in the pig, an omnivore whose dietary and dental development and occlusion patterns are similar to the human condition, and which, because of its size, is more readily amenable to experimental manipulation. The porcine junctional epithelium was also compared with this well-described epithelium in the rat. Morphological analyses by light microscopy and scanning and transmission electron microscopy showed the porcine junctional epithelium and epithelial attachment were similar to that in the rat except that apically, extracellular matrix lamellae associated with the internal basal lamina were more complex, and more coronally there was extensive layering of a dental cuticle-like material. Biochemical analysis of the porcine junctional epithelium by dissociative extraction and SDS-PAGE revealed the presence of some proteins not present in gingival epithelium. Together, these studies show that the porcine junctional epithelium has predictable morphological and biochemical features which establish the pig as an advantageous model to study the basic and clinical biology of this unique epithelium.

Animals↗
PubMed1989

[Gingivo-epithelial attachment in dental alloimplants].

In this article the structure of gingivo-epithelial tissue and survey of research about its attachment to the tooth neck are presented. Some further research themes in this important and delicate field of stomatologic implantology are also discussed.

Connective Tissue↗