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Biomedical subjects

A N Cheredeev

Publications and source records attributed to A N Cheredeev.

At least 73 records · Page 4Linked to original sources
PubMed1980

[Significance of the spontaneous rosette formation test with mouse erythrocytes in the complex quantitative analysis of human B-lymphocytes].

Some mechanisms were studied that are concerned with mouse red blood cell rosette formation by human peripheral blood lymphocytes. Lymphocytes rosetting with mouse red blood cells (EM--RFC) were identified by the test similar to the E-rosette formation technique. EM--RFC accounted for 7.7+/-0.48% in the peripheral blood of healthy donors. It has been established by stepwise selective inhibition of Fc- and C3-receptors and surface immunoglobulins that human Fc- and C3-receptors do not take part in rosetting with mouse red blood cells, whilst surface immunoglobulins, particularly IgM or near-located structures, are implicated in the reaction of lymphocytes with mouse red blood cells. Multiple analysis of lymphocyte receptors has been performed in patients with different immunologic disorders. A significant decrease in the EM--RFC level was found in patients with rheumatoid arthritis. In patients with dys-and hypoimmunoglobulinemia, the content of EM--RFC varied depending on the severity of immunity B-system injury and correlated well with the cells bearing surface immunoglobulins. The drastically increased number EM--RFC (up to 34.6--78%) was recorded in the blood of patients with chronic lympholeukemia. These findings served as basis for applying EM--RFC detection for diagnosing various forms of lympholeukemia.

Agammaglobulinemia↗
PubMed1980

[Methodologic features of the production of heterologous antisera to human T- and B-lymphocytes and evaluation of their specificity].

Antisera to human B-lymphocytes were obtained by immunization of rabbits with lymphocytes obtained from peripheral blood taken from healthy persons and patients having chronic lympholeukosis with B-cell proliferation. These antisera were subjected to adsorption on human erythrocytes, liver and thymocytes. The specificity of B-lymphocyte antiserum was evaluated in the E- and EAC-rosette-formation inhibition test and by the comparison of the number of lymphocytes revealed in the cytotoxic test with EAC-rosette-forming cells. Besides the above-mentioned tests, the specificity of rabbit antiserum to human thymocyte membranes was also determined in the test of inhibiting T-lymphocyte stimulation with phytohemagglutinin. The antisera thus obtained were specific, in definite dilutions, to human T- and B-lymphocytes.

Adsorption↗
PubMed1979

[Suppressive factor release by mouse lymphocytes after incubation with syngeneic erythrocytes].

Suppressive factor, inhibiting the immune response to sheep red blood cells, is released after incubation in vitro of spleen cells of (CBA X C57BL) F1 mice, immunized with rat erythrocytes, in contact with syngeneic erythrocytes. Intravenous immunization results in the factor release by thymus and/or spleen cells but not by the bone marrow or lymph node cells. It is suggested that the factor is not antigen-specific and is secreted by non-specific T cells suppressors after the contact with syngeneic erythrocytes.

Animals↗
PubMed1978

[Effect of phytohemagglutinin on T-lymphocytes isolated from human blood].

T--enriched lymphocyte fraction from normal donors was isolated from heparinized peripheral blood on Ficoll-Urotrast gradients. The mitogenic response of T-lymphocytes in culture was 3 times as high as that of unseparated mononuclear cells. The intensity of blast transformation to PHA did not correlate with the increase of T-lymphocytes in culture. These data suggest that cells of not T-origin may be the limiting factor during mitogenic response of T-lymphocytes to PHA.

Adult↗
PubMed1977

Distribution of HLA antigens in a Cuban population.

The frequencies of the HLA antigens were determined in a population of 160 unrelated Cubans from three different ethnic groups (white, mixed-blood and negro). We typed for antigenic specificities of the A and B loci, and also for the Cw1 specificity of the C locus. Phenotype and gene frequencies for the different ethnic groups are presented for comparison. The phenotype distribution, the haplotype frequencies for the white group and the genetic distance between this group and a Spanish population were calculated.

Cuba↗