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Biomedical subjects

A Zuckerman

Publications and source records attributed to A Zuckerman.

At least 73 records · Page 4Linked to original sources
PubMed1975

Neutralizing antibody in Rodent Malaria.

Small numbers of rat erythrocytes infected with viable P. berghei, when inoculated into susceptible rats together with hyperimmune rat serum (HIS), are fully neutralized. Serum from convalescent rats delays the onset of patency but does not neutralize. The neutralizing efficiency of HIS rises in proportion to the number of successive reinoculations of hyperimmune rats. In contrast, mice inoculated with parasites together with either HIS or normal rat serum succumbed to the disease at the same time after inoculation. Neutralization in rats occurs in vivo and is completed within 22 hours of inoculation. Much larger amounts of HIS are needed to achieve neutralization in splenectomized recipients than in intact rats. The action of HIS is dose-dependent. Thus, the degree of suppression of parasitaemia is proportional to the dose of HIS, while the mortality rate is inversely proportional to the dose. Suboptimal doses may even enhance the infection of recipient rats. The ability to produce neutralizing antibody is dissimilar in two strains of rat. Thus, the outbred Sabra strain produces neutralizing HIS, while the inbred Lewis rat is incapable of producing perceptible neutralizing antibody in our experimental model.

Animals↗
PubMed1975

Dynamics of thymidine incorporation by spleen cells from rats infected with Plasmodium berghei.

The in vitro incorporation of thymidine by spleen cells of rat infected with Plasmodium berghei was higher than that of normal rat spleen cells. The incorporation was proportional to the number of cells in the system and was related to the day of infection, e.g. spleen cells from malarious rats incorporated up to forty times as much thymidine as did normal spleen cells 21 days after inoculation. At the same time, the response of the malarious spleen cells to phytohaemagglutinin (PHA) was only one-twentieth of that observed for normal spleen cells. The thymidine was incorporated by non-adherent spleen cells in a single pulse during the first 8 hr in culture.

Animals↗
PubMed1969

Current status of the immunology of malaria and of the antigenic analysis of plasmodia. A five-year review.

The immunology of malaria has been intensively studied, and many reviews of separate topics have appeared. Among host factors contributing to susceptibility to malaria, the following are studied in the present paper: (1) genetic factors affecting susceptibility to human and rodent plasmodia; (2) physiological and nutritional factors affecting susceptibility of vertebrate and vector hosts; (3) sterile immunity in malaria as exemplified by radical cure and by modification of challenge infection following exposure to non-living parasite products; (4) the role of the lymphoid-macrophage system in malaria; and (5) the excessive anaemia of malaria and its etiology.Gamma-globulin levels rise in malaria and remain high during latency. Protection is associated with IgG, which is passively transferable via the human placenta. Not all gamma-globulin is antibody, and not all antibody is protective. The fluorescent antibody technique and double diffusion in gel have been extensively used in exploring the kinetics of antibody production.New methods of harvesting plasmodia attempt to avoid protein degradation and to minimize contamination by host antigens. Plasmodia have proven to consist of a mosaic of antigens, and comparative studies by most of the accepted techniques have been started. Exoantigens have been described in fowl- and rodent-malarias. Relapse-variants of primate plasmodia have been shown to differ antigenically from their parent strains.

Agglutination Tests↗
PubMed1967

A procedure for the harvesting of mammalian plasmodia.

Immunochemical research into the antigenic structure of a given disease agent presupposes the availability of undegraded antigen. Some types of immunochemical studies of plasmodia can be carried out with the intracellular parasites in situ in the host cell (for example, studies using the fluorescent antibody technique). In other techniques (such as double diffusion in gel, disc electrophoresis) the presence of host cell contaminants is undesirable, and these require to be reduced to a minimum.A method is described for harvesting mammalian (rodent, simian, and human) plasmodia. Plasmodia in the product are significantly concentrated as compared with the original samples. This point is particularly important in harvesting human plasmodia, in which parasitaemias tend to be very low. Significant reduction of red- and white-cell contaminants is achieved. Antigens in the cell-free plasmodial products obtained are apparently in their native state, and give replicable results in studies of double diffusion in gel, immunoelectrophoresis and disc electrophoresis, passive haemagglutination and vaccination.

Animals↗