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Biomedical subjects

B Bültmann

Publications and source records attributed to B Bültmann.

At least 55 records · Page 3Linked to original sources
PubMed1986

Experimental reovirus myocarditis in newborn mice. Electron microscopic observations.

Reovirus is a double-stranded RNA-virus which induces myocarditis in newborn mice. Due to the large diameter of the viral particles (70-75 nm) it can be detected by electron microscopy. Subcutaneous inoculation of 0.05 ml reovirus type 3 (TCID50-titer: 10(8.5)/ml) into newborn NMRI-mice (12-18 h after birth) caused a grey-yellow mottling on the ventricular surface first seen on the 5th day after birth. At the same time muscle fiber necrosis was observed which increased with time. Electron microscopic investigations of the diseased heart muscle disclosed a marked interstitial oedema, swelling of the tubular system and sarcoplasmic reticulum, and degenerative changes in the mitochondria of individual myocardiocytes as early as the 2nd post-inoculation day. Simultaneously, an enlarged Golgi-apparatus and an increasing number of lysosomes, partially exhibiting acid phosphatase activity, was detected in the perinuclear region of ventricular myocardiocytes. On the 5th day after infection, viruses were detected either within single membrane vesicles, dispersed in cytoplasm or as aggregated clusters in the perinuclear region. These in vivo electron microscopic findings correspond with observations of virus propagation in cell-culture systems.

Animals↗
PubMed1982

Critical analysis of granulocyte function in 154 patients wtih different diseases.

Several granulocyte functions were analyzed in vitro in 154 patients with chronic or recurrent infections, as well as in a variety of disorders known or suspected to affect host resistance. Only a few specific abnormalities were diagnostic and occurred in congenital, hereditary disorders. Opposed to these permanent changes are those which were probably acquired or transient and are often multifactorial in origin. In the majority of these patients, an inconstant and nonspecific pattern emerged which is not helpful in the diagnosis of underlying disease. In selected patients, however, and as research procedures, these and related tests should be helpful in elucidating the basic functions of granulocytes and may implicate therapeutical approaches.

Adolescent↗
PubMed1982

Selective inhibition of human neutrophilic chemotaxis by echo virus, type 9. Virus-induced changes in membrane fluidity.

By means of the excimer-forming lipid technique changes of membrane fluidity of human PMNs exposed to a chemoattractant (FMLP) and/or Echo virus, type 9, strain A. Barty has been investigated. It was shown that preincubation of PMNs with Echo 9 virus results in a viral dose-dependent decrease of membrane fluidity of granulocytes exposed to FMLP. The virus-induced rigidity of cell membrane causes obviously a disturbance in the aggregation of membrane particles to form receptors, and thus can lead to a failure of regular processing of chemotactic signals.

Chemotactic Factors↗
PubMed1981

Selective inhibition of human neutrophil chemotaxis by ECHO-virus, type 9.

Preincubation (37 degrees C, 60 min) of human neutrophilic granulocytes with various concentrations of infectious ECHO virus, type 9, strain A. Barty resulted in virus-dose dependent inhibition of neutrophil chemotactic reactivity (Boyden-chamber assay) to chemoattractants (F-Met-Leu-Phen: zymosan-activated human serum). Other cellular functions-phagocytosis, increase of oxidative cell metabolism, intracellular killing of live staphylococci--remained intact. This selective inhibition of human neutrophilic chemotaxis was due to viral induced cytotoxicity or competitive inhibition of chemotactic cell surface receptors.

Animals↗
PubMed1981

[Yersinioses. Clinical, patho-anatomical and pathogenetic problems (author's transl)].

On the basis of a selected patient group and bioptic material, the difficulties in diagnosing Y. pseudotuberculosis and Y. enterocolitica infections are presented from the clinical and morphological point of view, particularly when extraintestinal complications are present. Taking one patient with Y. enterocolitica infection and recurrent oligoarthritis as an example, the possible pathogenetic significance of circulating immune complexes for the development of extraintestinal symptoms is being discussed.

Adolescent↗